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Method for determining subcellular distribution of graphene in rice

A graphene and subcellular technology, applied in the direction of measuring devices, analytical materials, color/spectral characteristic measurement, etc., to achieve the effect of easy operation and simple method

Active Publication Date: 2019-01-11
NANJING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the main problem at present is that graphene enters the plant body, how to accurately quantify its distribution in subcellular cells in the body, which is useful for evaluating the influence of the presence of graphene in plants on the photoelectric transfer efficiency in plant photosynthesis and on plant growth. has important research significance

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  • Method for determining subcellular distribution of graphene in rice
  • Method for determining subcellular distribution of graphene in rice
  • Method for determining subcellular distribution of graphene in rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Graphene Centrifugal Sedimentation Efficiency and Graphene Enrichment Efficiency of Graphene with / without Cell Organelles

[0035] (1) Seed germination and graphene exposure: soak the newly harvested seeds in 30% H 2 o 2 solution for 15 min, and then washed three times with deionized water to ensure that the seed surface is sterile. The seeds were then soaked in deionized water and stored in the dark at 30°C for 48 h. Finally, the soaked seeds were evenly displayed in a sterilized petri dish, and the bottom of the petri dish was covered with two layers of sterilized moist filter paper, and the petri dish was covered and placed in a light incubator at 30°C for 7 days. After germination was complete, it was transferred to a container containing 2 L of exposure solution with a graphene concentration of 0 μg / L.

[0036] (2) After 7 days of exposure, rice leaves were collected, 0.2 g was accurately weighed, homogenized and centrifuged to obtain chloroplast compo...

Embodiment 2

[0041] Subcellular distribution results of graphene in rice stems under different exposure times in Example 2

[0042] (1) Seed germination and graphene exposure: soak the newly harvested seeds in 30% H 2 o 2 solution for 15 min, and then washed three times with deionized water to ensure that the seed surface is sterile. The seeds were then soaked in deionized water and stored in the dark at 30°C for 48 h. Finally, the soaked seeds were evenly displayed in a sterilized petri dish, and the bottom of the petri dish was covered with two layers of sterilized moist filter paper, and the petri dish was covered and placed in a light incubator at 30°C for 7 days. After germination was complete, it was transferred to a container containing 2 L of exposure solution with a graphene concentration of 250 μg / L. At the same time, another group of blank control group was set up, that is, after the germination ended, it was transferred to 2L of graphene-free solution.

[0043] (2) After expo...

Embodiment 3

[0048] Subcellular distribution results of graphene in rice leaves under different exposure times in Example 3

[0049] (1) Seed germination and graphene exposure: soak the newly harvested seeds in 30% H 2 o 2 solution for 15 min, and then washed three times with deionized water to ensure that the seed surface is sterile. The seeds were then soaked in deionized water and stored in the dark at 30°C for 48 h. Finally, the soaked seeds were evenly displayed in a sterilized petri dish, and the bottom of the petri dish was covered with two layers of sterilized moist filter paper, and the petri dish was covered and placed in a light incubator at 30°C for 7 days. After germination was complete, it was transferred to a container containing 2 L of exposure solution with a graphene concentration of 250 μg / L. At the same time, another group of blank control group was set up, that is, after the germination ended, it was transferred to 2L of graphene-free solution.

[0050] (2) After e...

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Abstract

The invention discloses a method for determining subcellular distribution of graphene in rice, and belongs to the application development of nanotechnology in the agricultural field. The method comprises the following steps: performing germination treatment on rice seeds, exposing graphene to germinated rice; respectively collecting stem and leaf parts of rice plants for fully grinding after beingcrushed and liquid nitrogen frozen; adding a pre-cooled homogenizing medium into the ground tissue to prepare a homogenate; separating the homogenate into five parts by differential centrifugation: cell wall, chloroplast, nucleus, mitochondria and soluble fraction; fully burning each component by a bio-oxidation instrument to collect carbon dioxide generated; and determining the grapheme contentin each component by a liquid scintillation counter. In order to evaluate the possible interaction between organelles and graphene during centrifugation, three control experiments are designed to accomplish this goal. The method achieves accurate quantification of graphene in subcellular of rice plants.

Description

technical field [0001] The patent of the present invention relates to a method for measuring the subcellular distribution of graphene in rice, which belongs to the application and development of nanotechnology in the field of agriculture. Background technique [0002] Graphene is a two-dimensional honeycomb lattice structure composed of a smooth single layer of carbon atoms and is the basic building block of other graphitic materials. Graphene can be wrapped to form zero-dimensional fullerenes, rolled to form one-dimensional carbon nanotubes, or stacked to form three-dimensional graphite. Since graphene was first exfoliated from graphite by Geim and Novoselov of the University of Manchester in 2004, due to its many extraordinary physical and chemical properties, such as high thermal conductivity, intrinsic strength, high electrical conductivity, high transparency, gas Due to its impermeability and easy functionalization, graphene research has developed rapidly, and graphene...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/31G01N23/00
CPCG01N21/31G01N21/33G01N23/00
Inventor 毛亮卢坤吴伟
Owner NANJING UNIV
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