Specific molecularly imprinted polymer for identifying chloramphenicol, chemiluminescent kit, detection method and application

A chemiluminescence reagent, molecular imprinting technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, material separation, etc., can solve the problem of increasing false positive result ratio, etc., achieve strong tolerance, reduce The effect of wasting resources and reducing inspection costs

Active Publication Date: 2019-01-11
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since these two congeners are not prohibited drugs for food animals and have allowable residue limits,

Method used

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  • Specific molecularly imprinted polymer for identifying chloramphenicol, chemiluminescent kit, detection method and application
  • Specific molecularly imprinted polymer for identifying chloramphenicol, chemiluminescent kit, detection method and application
  • Specific molecularly imprinted polymer for identifying chloramphenicol, chemiluminescent kit, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1 Synthesis Example 1 of Molecularly Imprinted Polymer

[0032] (A) Place 1 part of pseudo-molecular template nitrobenzene, 3 parts of functional monomer methacrylic acid, 20 parts of initiator azobisisobutyronitrile and 20 parts of crosslinking agent ethylene glycol dimethacrylate. React in chloroform solvent at 60°C for 12 hours to obtain solid particles;

[0033] (B) Put the solid particles in a Soxhlet extractor, and use methanol and acetic acid (9:1, V / V) extraction solution to reflux continuously for 12 hours to extract the imprinted pseudo-template molecule nitrobenzene;

[0034] (C) After drying the solid particles after the reaction in step (b), a specific molecularly imprinted polymer of chloramphenicol is obtained.

Example Embodiment

[0035] Example 2 Synthesis Example 2 of Molecularly Imprinted Polymer

[0036] (A) Place 1 part of pseudo-molecular template nitrobenzene, 6 parts of functional monomer methacrylic acid, 30 parts of initiator azobisisobutyronitrile and 30 parts of crosslinker ethylene glycol dimethacrylate React in chloroform solvent at 70°C for 10 hours to obtain solid particles;

[0037] (B) Put the solid particles in a Soxhlet extractor, and use methanol and acetic acid (9:2, V / V) extraction solution to reflux continuously for 24 hours to extract the imprinted pseudo-template molecule nitrobenzene;

[0038] (C) After drying the solid particles after the reaction in step (b), a specific molecularly imprinted polymer of chloramphenicol is obtained.

Example Embodiment

[0039] Example 3 Synthesis Example 3 of Molecularly Imprinted Polymer

[0040] (A) Place 1 part of pseudo-molecular template nitrobenzene, 4 parts of functional monomer methacrylic acid, 25 parts of initiator azobisisobutyronitrile and 25 parts of crosslinker ethylene glycol dimethacrylate. React in chloroform solvent at 65°C for 11 hours to obtain solid particles;

[0041] (B) Put the solid particles in a Soxhlet extractor, and use methanol and acetic acid (9:0.5, V / V) extraction solution to reflux continuously for 18 hours to extract the imprinted pseudo-template molecule nitrobenzene;

[0042] (C) After drying the solid particles after the reaction in step (b), a specific molecularly imprinted polymer of chloramphenicol is obtained.

[0043] The scanning electron micrograph of the molecularly imprinted polymer prepared in this example is as follows figure 2 Shown by figure 2 It can be seen that its surface is porous, and it is a cavity formed by template molecular imprinting, whi...

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Abstract

The invention discloses a specific molecularly imprinted polymer capable of identifying chloramphenicol, and a chemiluminescent kit with the specific molecularly imprinted polymer as an identificationcomponent, a detection method and application. Nitrobenzene is used as a pseudo-molecular template in the molecularly imprinted polymer, and the synthesized specific molecularly imprinted polymer canonly identify chloramphenicol. The chemiluminescent kit prepared based on the molecularly imprinted polymer can perform residue detection on chloramphenicol in meat tissues and can be reused, the detection sensitivity is improved, the detection time is shortened, and the detection cost is reduced.

Description

technical field [0001] The invention relates to the field of animal food safety, in particular to a molecularly imprinted polymer of chloramphenicol, a chemiluminescence kit, a detection method and an application. Background technique [0002] Chloramphenicol is a highly effective broad-spectrum antibiotic that has been widely used to treat various bacterial diseases in humans and animals. However, after chloramphenicol is used in food-producing animals, it will cause its residues in food of animal origin. After consumers take a small amount of chloramphenicol for a long time, it can lead to aplastic anemia in consumers, which is life-threatening. Therefore, the Ministry of Agriculture of China issued a notice in 2002 to prohibit the use of chloramphenicol in any form in the breeding process of food animals, and it must not be detected in all animal foods. However, due to its strong antibacterial effect, wide antibacterial spectrum and low price, it still has illegal use i...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N30/02G01N23/2251C08F220/06C08F222/14C08J9/26C08L33/02
CPCC08F220/06C08J9/26C08J2201/0424C08J2333/02G01N21/76G01N23/2251G01N30/02G01N2030/027C08F222/102
Inventor 王建平贾冰杰
Owner HEBEI AGRICULTURAL UNIV.
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