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Method for enhancing plant characteristics and method for producing seedless fruit

A production method and technology for seedless fruit, applied in the fields of botanical equipment and methods, plant regeneration, biochemical equipment and methods, etc., can solve problems such as low efficiency, and achieve the effect of enhancing growth characteristics and/or cold resistance

Inactive Publication Date: 2019-01-11
田中节三
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its efficiency is very low (about 5-10%)

Method used

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  • Method for enhancing plant characteristics and method for producing seedless fruit
  • Method for enhancing plant characteristics and method for producing seedless fruit
  • Method for enhancing plant characteristics and method for producing seedless fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] The characteristic enhancement of embodiment 1 papaya and the production of seedless fruit

[0138] In the state where the papaya seeds were soaked in the trehalose aqueous solution, they were frozen in a programmed cooling apparatus (freezing step). Slowly freeze at a rate of 0.5°C / day for 180 days, and the lowest temperature reaches -60°C during freezing.

[0139] The frozen papaya seeds were thawed by washing them with running water (thawing step), and the thawed seeds were sown and cultivated (growing step, first cultivation step). It is cultivated in Okayama Prefecture, Japan.

[0140] For the frozen and thawed treated seeds and untreated seeds, the growth conditions were observed along with the cultivation time, and the results were as follows: Figure 2-6 .

[0141] Such as Figure 2-6 As shown, compared with untreated papaya, the growth rate of papaya treated with freezing and thawing was significantly improved. This result shows that the growth characteristi...

Embodiment 2

[0149] The production of the seedless fruit of embodiment 2 papaya (2)

[0150] The stem tops of papaya obtained through the freezing step, the thawing step, the growing step and the first culturing step in Example 1 were collected and subjected to meristem clone culture (cultivation step). The individual seedlings of papaya developed through the cultivation step are cultivated in the open field (the second cultivation step).

[0151] Figure 7 , Figure 8 It shows the comparison photos of the flower buds formed in the second cultivation step and the flower buds of ordinary papaya, and the differences are clear at a glance. Such as Figure 7 As shown, it can be seen that the ovaries of the flower buds formed during the second cultivation step are enlarged. Also, if Figure 8 As shown, observing the section of the flower bud, it can be found that the flower bud produced in the process of the second cultivation step has resulted. Because there is no pollination in the frui...

Embodiment 3

[0154] The characteristic enhancement of embodiment 3 pineapple

[0155] The pineapple seeds are frozen and thawed in the same manner as in Example 1, and then sown and cultivated. The pineapple that has been frozen and thawed and the untreated pineapple are sown and cultivated at the same time, and the growth observed over time is recorded. The results are as follows: Figure 9 shown.

[0156] Such as Figure 9 Shown, like the papaya in embodiment 1, growth characteristics and cold resistance of pineapple processed through freezing and thawing are also enhanced.

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Abstract

Provided is a novel technology that enhances the characteristics of plants without using genetic recombination technology. A plant is treated with a freezing step for freezing a plant tissue, a thawing step for thawing the frozen plant tissue, and a growth step for growing a plant from the thawed plant tissue.

Description

technical field [0001] The present invention relates to a method for enhancing plant characteristics independent of genetic manipulation. The invention also relates to a technique for obtaining seedless fruit without relying on genetic manipulation and chemical treatment. Background technique [0002] Since ancient times, humans have bred plants with excellent properties through the method of variety improvement. The previous method of variety improvement often took a long time to make plants have certain characteristics. Later, due to the emergence of generation promotion technology, it became possible to shorten the time required for fixation characteristics. But even with generational enhancement, there is still the problem that it takes years for plants to fix certain traits. For this reason, biotechnology such as anther culture that does not require a fixation step is currently being developed. [0003] In addition, transgenic technology is known as a method of breed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00A01G2/30A01H3/00C12N5/04C12Q1/6895
CPCA01C1/00A01H4/008C12Q1/6895C12Q2600/13C12Q2600/158A01H3/00
Inventor 田中节三
Owner 田中节三
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