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A lysosome-targeted pH-sensitive nanoparticle and a preparation method and application thereof

A nanoparticle and lysosome technology, applied in the field of biomedicine, can solve the problems of inefficient aggregation of nanoparticles, poor tumor chemotherapy effect, low effective drug loading, etc., to achieve the best tumor chemotherapy effect, improve the aggregation degree and effectively The effect of drug loading and increasing lethality

Active Publication Date: 2019-01-15
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] One of the purposes of the present invention is to provide a lysosome-targeted pH-sensitive nanoparticle to solve the problem of poor tumor chemotherapy effect in the prior art due to inefficient aggregation of nanoparticles and low effective drug loading. question

Method used

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  • A lysosome-targeted pH-sensitive nanoparticle and a preparation method and application thereof
  • A lysosome-targeted pH-sensitive nanoparticle and a preparation method and application thereof
  • A lysosome-targeted pH-sensitive nanoparticle and a preparation method and application thereof

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Embodiment Construction

[0036] The following is further described in detail through specific implementation methods:

[0037] One, the preparation method of the lysosome-targeted pH-sensitive nanoparticles of the present invention, the specific steps are as follows:

[0038] (1) Preparation of silk protein liquid

[0039] Put the cocoon in Na 2 CO 3 Boil in sodium carbonate (0.02M) solution for 1h, then replace with fresh Na 2 CO 3 The solution was boiled again for 1 hour, and then the silkworm cocoons were soaked in deionized water, and the water was changed 4-5 times in the middle to wash off the sericin protein on the surface of the silkworm cocoon; then the degummed silk protein was dissolved in LiBr (9.2M) solution of lithium bromide at 60°C After 5 hours, transfer to deionized water and dialyze for 3 days to remove impurities and other ions, and finally filter and collect the silk protein liquid, freeze-dry for later use;

[0040] (2) Preparation of nanoparticles

[0041] Dissolve 5 mg of d...

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Abstract

The invention relates to the technical field of biomedical technology, and discloses lysosome-targeted pH-sensitive nanoparticle, comprising adriamycin calcium complex Ca-DOX cluster formed by chelating calcium ions Ca<2+> with adriamycin DOX. Fibroin is attached to the surface of the adriamycin calcium complex Ca-DOX cluster. The invention can enable more nanoparticles to be swallowed into the lysine body by cells, the aggregation degree and effective drug loading of nanoparticles in tumor region are improved, and accelerated the decomposition of nanoparticles in lysosomes, so that more Ca<2+> and CO2 are released, adriamycin can be released from the lysosome and enter the cell nucleus to play an anti-tumor role, thus effectively improving the lethality of the tumor, achieving the effectof tumor treatment, and providing a new direction for the clinical treatment of tumor.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a lysosome-targeted pH-sensitive nanoparticle and a preparation method and application thereof. Background technique [0002] Cancer is still one of the most important diseases affecting human health. According to U.S. cancer statistics, there were 1,660,290 new cases and 580,350 deaths in 2013 alone. At present, chemotherapy is still the main clinical treatment for tumors, but due to the dose-limiting toxicity and instability of chemotherapy drugs, the effect of chemotherapy is poor and cannot meet the clinical needs. Therefore, high-efficiency drug carriers have been developed to allow chemotherapy drugs to be released quickly after entering tumor cells, so as to achieve the purpose of killing tumor cells, thereby exerting anti-tumor effects. [0003] In recent years, nano-drug delivery system has been gradually applied to tumor chemotherapy, but the chemotherapy effect i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K31/704A61K47/52A61K47/64A61P35/00
CPCA61K9/0019A61K9/1611A61K9/1658A61K31/704A61P35/00
Inventor 谭米肖冉海涛曹阳过源郭丹刘逢秋刘薇薇陈瑜莉杨超
Owner CHONGQING MEDICAL UNIVERSITY
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