Method for preparing methylene- blue-containing silica/shellac composite particles with high fluorescence intensity by auxiliary heat treatment

A technology of methylene blue and silicon dioxide, applied in chemical instruments and methods, luminescent materials, etc., to achieve the effects of good biocompatibility, low price, and simple process methods

Inactive Publication Date: 2019-01-15
SHANDONG UNIV OF TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the method of preparing silica / shellac composite particles containing methylene blue with high fluorescence intensity by heat-treating silica particles containing methylene blue has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing methylene- blue-containing silica/shellac composite particles with high fluorescence intensity by auxiliary heat treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Add 92 ml of absolute ethanol, 17.2 ml of water, 2.48 ml of concentrated ammonia water and 0.10 g of methylene blue to the beaker in sequence. After stirring for 15 minutes, add 3.44 ml of ethyl orthosilicate. After stirring for 4 hours, centrifuge to obtain the sediment , the precipitate was first washed twice with 120 ml of ethanol, and then washed once with 20 ml of acetate buffer solution of pH = 3.6, and then the sediment obtained by centrifugation was dried at 70°C for 24 hours to obtain the Silica particles of methylene blue;

[0016] (2) Dissolving shellac in ethanol to prepare a shellac solution, wherein the concentration of shellac is 10 grams per liter, put the silica particles containing methylene blue prepared in step (1) into 10 ml of shellac In the solution, the concentration of silica particles containing methylene blue was 10 grams per liter, and after ultrasonic treatment for 5 minutes, 30 milliliters of phosphate buffer (pH=7.2~7.4) was added at a...

Embodiment 2

[0019] (1) Add 92 ml of absolute ethanol, 17.2 ml of water, 2.48 ml of concentrated ammonia water and 0.10 g of methylene blue to the beaker in sequence. After stirring for 15 minutes, add 3.44 ml of ethyl orthosilicate. After stirring for 4 hours, centrifuge to obtain the sediment , the precipitate was first washed twice with 120 ml of ethanol, and then washed once with 20 ml of acetate buffer solution of pH = 3.6, and then the sediment obtained by centrifugation was dried at 70°C for 24 hours to obtain the Silica particles of methylene blue;

[0020] (2) Dissolving shellac in ethanol to prepare a shellac solution, wherein the concentration of shellac is 10 grams per liter, put the silica particles containing methylene blue prepared in step (1) into 10 ml of shellac In the solution, the concentration of silica particles containing methylene blue was 10 grams per liter, and after ultrasonic treatment for 5 minutes, 30 milliliters of phosphate buffer (pH=7.2~7.4) was added at a...

Embodiment 3

[0023] (1) Add 92 ml of absolute ethanol, 17.2 ml of water, 2.48 ml of concentrated ammonia water and 0.10 g of methylene blue to the beaker in sequence. After stirring for 15 minutes, add 3.44 ml of ethyl orthosilicate. After stirring for 4 hours, centrifuge to obtain the sediment , the precipitate was first washed twice with 120 ml of ethanol, and then washed once with 20 ml of acetate buffer solution of pH = 3.6, and then the sediment obtained by centrifugation was dried at 70°C for 24 hours to obtain the Silica particles of methylene blue;

[0024] (2) Dissolving shellac in ethanol to prepare a shellac solution, wherein the concentration of shellac is 10 grams per liter, put the silica particles containing methylene blue prepared in step (1) into 10 ml of shellac In the solution, the concentration of silica particles containing methylene blue was 10 grams per liter, and after ultrasonic treatment for 5 minutes, 30 milliliters of phosphate buffer (pH=7.2~7.4) was added at a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing methylene- blue-containing silica / shellac composite particles with high fluorescence intensity by auxiliary heat treatment, and belongs to the technical field of chemical raw material preparation. The method is as follows: adding ammonia water, methylene blue and ethyl orthosilicate to a mixed solution of water and ethanol, stirring for 4 hours, centrifuging, washing, drying at 70 DEG C, dispersing prepared methylene-blue-containing silica particles in a shellac solution, adding a phosphate buffer (pH=7.2-7.4) dropwise, stirring for 1 hour, centrifuging, washing and drying at room temperature, and performing heat treatment on prepared methylene- blue-containing silica / shellac composite particles to obtain the methylene- blue-containing silica / shellac composite particles with high fluorescence intensity. The method has the advantages of simple process, low raw material price and easy industrial production, and the obtained particles have good biocompatibility and high fluorescence intensity.

Description

technical field [0001] The invention discloses a method for assisting the preparation of methylene blue-containing silica / shellac composite particles with high fluorescence intensity through heat treatment, which belongs to the technical field of chemical raw material preparation. Background technique [0002] Dye molecules have high application value in fluorescence sensors, bioluminescence labeling, cell tracking and solid-state lasers, etc. The realization of these properties is related to the aggregation state of dye molecules. In high-concentration solutions or solid powders, dye molecules, such as methylene blue molecules, are easy to form aggregates, and the interaction between aggregated molecules will lead to the occurrence of fluorescence quenching effect, which will weaken the fluorescence signal, and then cause the fluorescence signal of methylene blue to be almost Unable to detect. Only in dilute aqueous solutions, such as concentrations of methylene blue molec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09K11/02C09K11/06
CPCC09K11/025C09K11/06C09K2211/1037
Inventor 李成峰张伟王文浩王杰葛筱璐
Owner SHANDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap