Immune thrombocytopenia lncRNA marker, kit and application

A technology of thrombocytopenia and markers, applied in the field of biomedical diagnosis, can solve problems such as abnormal expression of lncRNA, and achieve the effect of auxiliary diagnosis of prognosis, easy operation and high sensitivity

Inactive Publication Date: 2019-01-15
中国人民解放军第一OO医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The latest research has confirmed that lncRNA-TMEVPG1 can act as an enhancer to promote the transcription of interferon gamma (IFNG), and is related to the expression of Th1-type transcription factors T-bet, STAT1 and STAT4, and plays an important role in the pathogenesis of immune thrombocytopenia, but currently Research on lncRNAs in immune diseases is still in its infancy, and which lncRNAs are abnormally expressed are still controversial

Method used

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  • Immune thrombocytopenia lncRNA marker, kit and application
  • Immune thrombocytopenia lncRNA marker, kit and application
  • Immune thrombocytopenia lncRNA marker, kit and application

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Experimental program
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Effect test

Embodiment 1

[0040] 1. Collection of clinical samples

[0041] The inventor has collected a large number of patients with immune thrombocytopenia from the 100th Hospital of the People's Liberation Army and the Affiliated Hospital of Soochow University since 2016. The diagnostic criteria are all in line with the "Chinese Expert Consensus on the Diagnosis and Treatment of Primary Immune Thrombocytopenia in Adults (Revised Edition)"; At the same time, the healthy positive control group in the physical examination center was selected, and peripheral blood was drawn. This study was approved by the Hospital Ethics Committee, and all subjects signed informed consent. The process of collection and follow-up experiments complied with the requirements of medical ethics and strictly followed the principle of confidentiality of case data. The sampling, packaging and storage conditions of the research samples were the same. Through the analysis and arrangement of medical records, the inventor selecte...

Embodiment 2

[0102] ROC curve was used to analyze the diagnostic value of LOC100506314 level for ITP. Among the 120 test objects, the ITP patients were used as the target objects, and the ROC curve was made by Graphpad software, and various related parameters were analyzed. like Figure 4 As shown, the area under the curve reaches 0.918±0.024 (P<0.001), indicating that it has very good diagnostic value. Using the principle of the largest Youden’s index, the optimal critical value of LOC100506314 was judged to be 1.971, and the corresponding sensitivity and specificity were 73.75 and 97.5%, respectively, as shown in Table 2.

[0103] Table 2:

[0104]

Embodiment 3

[0106] ROC curve was used to analyze the predictive value of peripheral blood LOC100506314 level on clinical stage of ITP patients. Among the 80 ITP patients, the patients with chronic ITP and refractory period were taken as the target objects, and the ROC curve was drawn by using Graphpad software, and various related parameters were analyzed. like Figure 5 As shown, the area under the curve reaches 0.949±0.021 (P Image 6 As shown, the area under the ROC curve reached 0.958±0.026 (P<0.001), indicating that it has a good predictive value for newly diagnosed ITP patients. Using the principle of the largest Youden index, the optimal critical value of LOC100506314 was judged to be 2.310, and the corresponding sensitivity and specificity were 80% and 100%, respectively.

[0107] In summary, the peripheral blood lncRNA marker LOC100506314 of the present invention was significantly increased in immune thrombocytopenia, and the expression level was significantly negatively correlat...

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Abstract

The invention provides an immune thrombocytopenia lncRNA marker, a kit and an application thereof. The lncRNA marker is LOC100506314, and its base sequence is shown in SEQ ID NO: 1. The base sequenceof the primer of the lncRNA marker is shown in SEQ ID NO: 2 and SEQ ID NO: 3. LncRNA markers and primers thereof are used for preparing drugs for inhibiting immune thrombocytopenia or for preparing immune thrombocytopenia auxiliary diagnostic kits. The lncRNA marker can be used for judging the progress and severity of immune thrombocytopenia. The LOC100506314 primer and diagnostic reagent kit anddetection method developed on the basis of LOC100506314 are easy to operate, less invasive, high sensitivity and specificity, and can be used to monitor the disease progression clinically. The LOC100506314 primer and diagnostic reagent kit has an auxiliary diagnostic effect on the disease onset, drug efficacy and prognosis, and has obvious clinical application value.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and in particular relates to an immune thrombocytopenia lncRNA marker, a kit and an application. Background technique [0002] Primary immune thrombocytopenia (primary immune thrombocytopenia, ITP) is an autoimmune disease in which patients produce autoantibodies against platelet-specific glycoproteins GPIIb-IIIa and GPIb-IX, which in turn trigger monocyte / macrophage Cellular Fc receptor-mediated clearance of platelets, resulting in a decrease in platelet numbers. It is characterized by decreased platelet production and increased destruction, clinical bleeding is the main cause, and the risk of bleeding increases with age. The etiology of ITP is very complex, involving many factors including genetics, infection, and immune dysfunction. It is generally believed that the pathogenesis of ITP is mainly based on immunology. The treatment of ITP is mostly symptomatic. The progression of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6883C12Q2600/136C12Q2600/158C12Q2600/166C12Q2600/178C12Q2531/113C12Q2545/101
Inventor 钱琤吴天勤任传路邓安梅严文颖沈红石李征洋
Owner 中国人民解放军第一OO医院
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