The method and application of obtaining the extract of Chonglou
A technology of extracting solution and Chonglou, which is applied in the field of obtaining Chonglou extract, establishing the characteristic map of Chonglou, and evaluating the quality of Chonglou, which can solve the problem of affecting the quality and curative effect of Chonglou medicinal materials and related finished medicines, and failing to meet the quality standard requirements , can not guarantee the safety and effectiveness of medication and other issues
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[0069] 4. Preparation of reference solution
[0070] Precisely weigh 3.36mg of uridine, 2.77mg of guanosine, 4.77mg of adenosine, 4.95mg of papaya saponin VII, 4.97mg of diosgenin, 4.76mg of papaya saponin I and 8.76mg of papaya saponin V, and put them in a 10mL measuring bottle. Add distilled water to dissolve and dilute to the mark, shake well and set aside.
Embodiment 1
[0071] Example 1 Preparation of Chonglou Extract
[0072] Take 0.5g of Chonglou powder (passed through No. 3 sieve), accurately weigh 4 parts, put it in a 10mL centrifuge tube, add 5mL each of water, 30% ethanol, 90% ethanol and methanol solution, weigh, mix well, and extract by ultrasonic After 30 minutes, take it out, leave it at room temperature, and use the corresponding extraction solvent to make up the weight, centrifuge at 5000rpm for 10 minutes, take the supernatant and pass it through a 0.22 μm microporous membrane to obtain the product.
[0073] Take another 0.5mL alcohol extract supernatant, add 0.5mL water extract supernatant, mix evenly, and pass through a 0.22μm microporous membrane to obtain alcohol extract + water extract.
[0074] Take another 0.5mL of the supernatant of the alcohol extract, evaporate it to dryness, add 0.5mL of water for ultrasonic reconstitution, then add 0.5mL of the supernatant of the water extract, mix well, and pass through a 0.22μm microp...
Embodiment 2
[0079] Embodiment 2 chromatographic conditions investigation
[0080] Welch Ultimate AQ-C18 column (4.6×150mm, 5μm), mobile phase is acetonitrile-water, gradient elution, wavelength switching detection, injection volume 15μL, flow rate 1.0mL / min, column temperature 30°C. Among them, gradient elution and wavelength switching detection conditions are as follows:
[0081] Condition 1: 0~7min, 0% acetonitrile; 7~15min, 0%→3% acetonitrile; 15~20min, 3%→30% acetonitrile; 20~40min, 30%→45% acetonitrile; 40~60min, 45% →50% acetonitrile; 60~65min, 50% acetonitrile; 65~70min, 50%→0% acetonitrile; 70~75min, 0% acetonitrile. Detection wavelength: 0~30min, 260nm; 30~75min, 203nm.
[0082] Condition 2: 0~7min, 0% acetonitrile; 7~20min, 0%→3% acetonitrile; 20~25min, 3%→20% acetonitrile; 25~35min, 20%→30% acetonitrile; 35~60min, 30% →45% acetonitrile; 60~80min, 45→50% acetonitrile; 80~85min, 50%→0% acetonitrile; 85~90min, 0% acetonitrile. Detection wavelength: 0~40min, 260nm; 40~90min, 20...
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