Microsphere controllable equipment based on optical tweezers and operation method

Abstract

The invention relates to microsphere controllable equipment based on optical tweezers and an operation method. The microsphere controllable equipment based on the optical tweezers comprises an opticaltweezers system, a sample pond and an illumination imaging light path. The operation method comprises the following steps: initializing the optical tweezers system; preparing a mixed solution capableof separating out microspheres; uniformly mixing a phosphate buffer solution and isopropanol according to a volume ratio of 1:(3.5-6); injecting the mixture into the sample pond; placing the sample pond on a sample platform of the optical tweezers system; locally heating the phosphate buffer solution and isopropanol mixed solution by using a highly converged laser spot, so that phosphate crystalsnear the spot are separated out to form phosphate microspheres; observing the prepared phosphate microspheres by using the illumination imaging light path; and controlling and operating sizes, preparation speeds and positions of phosphate crystal particles by adjusting laser light intensity, a laser light beam direction and a laser light illumination time.

Description

technical field [0001] The invention relates to a method for controllably preparing and manipulating microspheres using an optical tweezers system. In particular, it relates to the field of three-dimensional optical trap system and particle crystallization produced by highly focused collimated laser beams. Background technique [0002] Optical tweezers use the three-dimensional potential well formed by highly concentrated laser beams to stably capture micron-scale particles near the focal point. It is widely used by scholars in the fields of cell biology, single-molecule biology, physics, and colloid science to capture and manipulate cells in ear capillaries of living mice to clear blood vessel blockages, capture polystyrene / silica microscopic The ball further studies the protein unfolding / folding kinetics and so on. However, the above studies are all based on the capture and manipulation of micron-scale particles by optical tweezers. The powerful functions of optical twee...

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Problems solved by technology

[0004] Most of the optical tweezers in the above studies focus on the interface between liquid and air, but there are very few studies on the preparation of microparticles by focusing inside the solution; in addition, the above preparation techniques or under the supersaturated state of a single solution Or a solution system that is undergoing a chemical reaction, that is to say, the scope of application is not wide enough

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