Method for tumor marker quantitative determination through metal-wrappage -antibody composite nano particle based on raman enhancement
A technology of composite nanoparticles and metal nanoparticles, applied in the field of physics, can solve problems such as measurement, easy interference, and cumbersome sample processing, and achieve the effects of obvious technical effects, high sensitivity, and huge application potential
Active Publication Date: 2019-01-22
上海镭立激光科技有限公司
View PDF13 Cites 3 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] The object of the present invention is to provide a method for the quantitative detection of tumor markers based on Raman-enhanced metal-encapsulation-antibody composite nanoparticles, which is based on the Raman-enhanced metal-encapsulation-antibody composite nanoparticles for quantitative detection of tumors The marker method should solve the technical problems of tedious sample processing, easy interference, inability to quantify and inability to perform multi-channel tumor marker measurement on biological samples in the prior art
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0030] Such as figure 1 , 2 As shown in and 3, the method for the quantitative detection of tumor markers based on Raman-enhanced metal-coating-antibody composite nanoparticles of the present invention includes three processes:
[0031] 1: The preparation process of metal-encapsulation-antibody composite nanoparticles is:
[0032]First, measure 60mL of 0.01mol / L silver nitrate solution with a graduated cylinder, add ammonia to make silver ammonia solution, then weigh 0.1g polyvinylpyrrolidone (PVP) and dissolve it in the silver ammonia solution; Dissolve 0.21g of ascorbic acid in 60mL of water to form an ascorbic acid solution, and add 0.01g of hydrazine hydrate to the solution. Finally, the prepared silver ammonia solution and ascorbic acid solution were simultaneously added dropwise to a 500 mL beaker with magnetic stirring at 35 degrees. Continue to age for 15 minutes after the reduction reaction ends, and prepare silver particles with an average particle diameter of 50 ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Login to view more
Abstract
The invention discloses a method for tumor marker quantitative determination through a metal-wrappage -antibody composite nano particle based on raman enhancement. The method comprises the following steps of: dispersing a metal nano particle included in an inorganic substance or an organic substance or a polymer or a precious metal into a buffer solution and adding an antibody to realize reaction,enabling the antibody to wrap the metal nano particle, thereby obtaining the metal-wrappage -antibody composite nano particle, and dispersing the metal-wrappage -antibody composite nano particle in the buffer solution after washing; and repeatedly preparing various metal-wrappage -antibody composite nano particle solution containing different antibodies and having different concentrations, and uniformly mixing the solution; mixing purified liquid containing a tumor marker with the nano particle mixed solution, after reaction, obtaining a new metal-wrappage -antibody-antigen composite nano particle formed after specific binding of the antibody and the antigen, then, performing transmission spectrum detection, and obtaining concentration of various tumor markers. The method provided by theinvention has the advantages of being simple to operate and highly sensitive and having wide detection intervals and strong specificity. The method can simultaneously perform quantitative analysis forvarious tumor markers, and has great significance in early diagnosis of tumor.
Description
technical field [0001] The present invention relates to the field of physics, in particular to optical detection technology, in particular to Raman spectroscopy detection technology, in particular to a method for quantitative detection of tumor markers based on Raman-enhanced metal-encapsulation-antibody composite nanoparticles. Background technique [0002] At present, cancer has seriously threatened human health and has become one of the leading causes of death. The World Cancer Report 2014 released by the World Health Organization (WHO) estimates that the number of cancer deaths worldwide will reach 13 million in 2030. At the same time, the data provided by the World Health Organization also shows that one-third of cancer patients can be cured through early detection and early treatment. For example, the 5-year survival rate of stage I breast cancer, nasopharyngeal cancer and laryngeal cancer is close to 100%. However, the patients found by clinical testing are often in ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 龚辉倪勤朱宝强苏江文
Owner 上海镭立激光科技有限公司
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap