Breathing bottle, measuring device and measuring method for measuring biofilm respiration rate

A technology of respiration rate and measurement device, applied in the direction of supporting/immobilizing microorganisms, biochemical cleaning device, biochemical equipment and method, etc., can solve problems such as inability to achieve measurement, interfere with magnetic stirring bar, and affect measurement results, etc., to achieve Accurate and true understanding of growth status, accurate and effective measurement results, and simple measurement process

Active Publication Date: 2022-04-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is to directly put the filler into the breathing bottle, and match it with magnetic stirring, so as to conduct a breath dynamics test; during the test, the filler will interfere with the rotation of the magnetic stirrer and affect the oxygen mass transfer; in addition, the magnetic stirrer will put the filler on the The biofilm is scraped off, thereby destroying the biofilm structure and affecting the measurement results; in fact, the measurement cannot be realized
The other is to directly centrifuge the MBBR filler to make the biofilm on the filler fall off, suspend it in water, and then carry out the respiratory dynamics experiment according to the conventional method; this method has caused irreversible damage to the biofilm on the filler; At the same time, the shedding of the biofilm leads to changes in the mass transfer rate of oxygen on the surface of the filler biofilm, which cannot truly reflect the respiratory dynamics of the biofilm.

Method used

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  • Breathing bottle, measuring device and measuring method for measuring biofilm respiration rate
  • Breathing bottle, measuring device and measuring method for measuring biofilm respiration rate
  • Breathing bottle, measuring device and measuring method for measuring biofilm respiration rate

Examples

Experimental program
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Effect test

Embodiment 1

[0037] figure 1 It is the schematic diagram of the respirator composition measuring device that is connected to the breather bottle for measuring the oxygen absorption rate of the embodiment 1 for measuring the biofilm respiration rate, figure 2 It is the top view of the breathing bottle of embodiment 1. like Figure 1-2 Shown, a kind of breathing bottle that is used for measuring biofilm respiration rate of the present invention comprises bottle body 1, carbon dioxide absorption tube 11 and the perforated partition 6 that is arranged on bottle body 1 inside; Divided into an oxygen mass transfer stirring zone 8 and a biofilm reaction zone 3, the oxygen mass transfer stirring zone 8 is provided with a stirring device 7, and the biofilm reaction zone 3 is used to place MBBR filler 2; the carbon dioxide absorption pipe 11 is provided with a vent hole 10 and contains Capable of absorbing CO 2 KOH solution to absorb CO in bottle 1 2 The perforated partition 6 has a first hole ...

Embodiment 2

[0047] Figure 4 The schematic diagram of the respirator composition measuring device for measuring the oxygen absorption rate connected to the breathing bottle for measuring the biofilm respiration rate of embodiment 2 and embodiment 3, Figure 5 It is the top view of the breathing bottle of embodiment 2. This embodiment is the second embodiment of the breathing bottle of the present invention, as Figure 4-5 As shown, the difference from Example 1 is: the bottle body 1 in this embodiment is a cylinder; the perforated partition 6 is cylindrical, and the inside of the cylindrical perforated partition 6 is an oxygen mass transfer stirring zone 8, and the cylinder Between the outside of the shaped perforated partition 6 and the bottle body 1 is the biofilm reaction zone 3; the perforated partition 6 is located in the middle of the bottle body 1, and the first bottleneck corresponds to the oxygen mass transfer stirring zone inside the cylindrical perforated partition 8. The bot...

Embodiment 3

[0050] Figure 4 The schematic diagram of the respirator composition measuring device for measuring the oxygen absorption rate connected to the breathing bottle for measuring the biofilm respiration rate of embodiment 2 and embodiment 3, Image 6 It is the top view of the breathing bottle of embodiment 3. This embodiment is the second embodiment of the breathing bottle of the present invention, as Figure 4 and Image 6 As shown, the difference from Example 1 is that there are two perforated partitions 6, an oxygen mass transfer stirring zone 8 between the two perforated partitions 6, and a biofilm between the perforated partition 6 and the bottle body 1. Reaction zone 3; bottle body 1 is provided with two second bottlenecks, and described two second bottlenecks correspond to two biofilm reaction zones 3 respectively; Described two second bottlenecks are respectively provided with the seal that is used for sealing bottle cap13.

[0051] The CO released by the microbial rea...

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PUM

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Abstract

The invention discloses a breathing bottle for measuring the respiration rate of biofilm, which comprises a bottle body, a carbon dioxide absorption tube and a perforated partition arranged inside the bottle body; Stirring area and biofilm reaction area for placing MBBR filler; carbon dioxide absorption pipe is provided with air holes and contains 2 solution; the perforated partition has a first hole corresponding to the middle and lower parts of the perforated partition and used as a liquid exchange channel and a second hole corresponding to the upper part of the perforated partition and used as a gas exchange channel, and the oxygen mass transfer stirring zone passes through the liquid exchange channel The gas exchange channel communicates with the biofilm reaction zone. On the one hand, it ensures that the gas-liquid exchange in the oxygen mass transfer stirring zone and the biofilm reaction zone is fully carried out; Respiratory dynamics characteristics. The determination process is simpler and faster, and the determination result is more accurate and effective.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a breathing bottle, a measuring device and a measuring method for measuring the respiration rate of a biofilm. Background technique [0002] Fluidized bed biofilm reactor (MBBR) is a new type of biofilm reactor, which is an improved new composite biofilm developed on the basis of fixed bed reactor, fluidized bed reactor and biofilter. reactor. The process requires the use of suspended fillers to provide a suitable growth environment for microorganisms. Determining the respiratory dynamics characteristics of biofilm on MBBR suspended packing is extremely important for optimizing the design and operation of sewage treatment process. [0003] There are two existing methods for measuring the respiration dynamics of biofilms on MBBR fillers. One is to directly put the filler into the breathing bottle, and match it with magnetic stirring, so as to conduct a breath dynamics test; duri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/24C12M1/04C12M1/02C12M1/00C12Q1/02
CPCC12M23/08C12M23/34C12M23/38C12M25/02C12M27/04C12M29/04C12M41/46C12Q1/02C12Q2304/44Y02W10/10C12M25/18C12M25/20C12M41/34C12M41/48C02F2209/36C02F3/085C12M29/20
Inventor 刘国强卢子川
Owner JINAN UNIVERSITY
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