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Method for repairing arsenic pollution soil by nano zero-valent iron and bacteria

A nano-zero-valent iron and arsenic pollution technology, applied in the restoration of contaminated soil, etc., can solve the problems of reducing activity, soil biological toxicity, increasing costs, etc., and achieve the effect of avoiding damage and reducing dosage

Active Publication Date: 2019-01-29
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When nanometer zero-valent iron is used alone to remediate arsenic-contaminated soil, the dosage is generally 5-10%, which will not only increase the cost, but also may cause certain biological toxicity to the soil
In addition, nano-sized zero-valent iron is easily oxidized in the air, forming a passivation layer and reducing the activity.

Method used

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  • Method for repairing arsenic pollution soil by nano zero-valent iron and bacteria
  • Method for repairing arsenic pollution soil by nano zero-valent iron and bacteria
  • Method for repairing arsenic pollution soil by nano zero-valent iron and bacteria

Examples

Experimental program
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Effect test

Embodiment example 1

[0027] 1) Enrichment culture of bacterial strains

[0028] Pseudomonas putida P. putida strain MnB1, strain MnB1 from the American Type Bacteria Collection Center, accession number ATCC 23483, inoculate the bacteria into Pseudomonas putida enrichment medium A at a volume ratio of 5%, and then shake at 30℃. (150 rpm), aerobic enrichment culture for 3 days; the composition of medium A is peptone 5g, beef powder 2, sodium chloride 3, pH 7.2, deionized water 1 liter;

[0029] 2) Preparation of active metabolites

[0030] Add the Pseudomonas putida bacteria solution obtained in step 1) to the inoculum of 5% by volume with the initial concentration of divalent manganese of 20 mg / L (dosing amount is calculated as Mn), and the odor of 6.8 Monosporium culture medium B, then cultured at 30 degrees Celsius under aerobic conditions for 2 days, brown-black flocs appear in the culture medium, that is, bacterial metabolites are produced; the composition of medium B is ferrous ammonium sulfate 0.3g...

Embodiment example 2

[0037] 1) Enrichment culture of bacterial strains

[0038] The selected bacterial strain is Pseudomonas putida P. putida strain MnB1, strain MnB1 from the American Type Bacteria Collection Center, deposit number ATCC 23483, inoculate the bacteria in Pseudomonas putida enrichment medium A at a volume ratio of 10%, and then shake at 25℃. (150 rpm), aerobic enrichment culture for 5 days; the medium A component is peptone 5g, beef powder 5, sodium chloride 3, pH 7.4, deionized water 1 liter;

[0039] 2) Preparation of active metabolites

[0040] Add the Pseudomonas putida bacteria solution obtained in step 1) to the inoculum of 10% by volume, with the initial concentration of divalent manganese of 5mg / L (dosing amount is calculated as Mn) and the foul-smelling pseudomonas with a pH of 7.4 Cell culture medium B, and then cultured for 7 days under aerobic conditions at 25 degrees Celsius, brown-black flocs appear in the medium, that is, bacterial metabolites are prepared; the composition ...

Embodiment example 3

[0047] 1) Enrichment culture of bacterial strains

[0048] Pseudomonas putida P. putida Strain MnB1 was inoculated into Pseudomonas putida enrichment medium A at a volume ratio of 7%, and then aerobic enrichment culture was carried out for 3 days under 30°C horizontal shaking (120 rpm); the medium A The ingredients are peptone 4g, beef powder 2, sodium chloride 2, pH 7.2, and 1 liter of deionized water. The selected bacterial strains are from the American Type Bacteria Collection Center and the deposit number is ATCC 23483;

[0049] 2) Preparation of active metabolites

[0050] Add the Pseudomonas putida bacteria solution obtained in step 1) to the inoculum of 8% by volume with the initial concentration of divalent manganese of 15 mg / L (dosing amount is calculated as Mn) and the malodorous phlegm with pH 7.0 Monosporium culture medium B, then cultured at 25 degrees Celsius under aerobic conditions for 3 days, brown-black flocs appear in the culture medium, that is, bacterial metabol...

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Abstract

The invention discloses a method for repairing arsenic pollution soil by nano zero-valent iron and bacteria. The method for repairing the arsenic pollution soil by the nano zero-valent iron and the bacteria comprises the following steps that Pseudomonas putida strain Mn B1 (ATCC 23483) is selected, the strain is enriched and cultured in an enrichment medium, the strain is inoculated into a culturemedium containing divalent manganese to be cultured to obtain active metabolites, the active metabolites and green synthesized nano zero-valent iron are added into arsenic pollution soil, and uniformly stirring is carried out to enable active manganese oxides, the zero-valent iron and trivalent arsenic or pentavalent arsenic in the soil to generate a series of physics-chemical reactions, and arsenic in soil can be effectively fixed. The method for repairing the arsenic pollution soil by the nano zero-valent iron and the bacteria has the advantages of short repair time, high efficiency, stableeffect, large treatment range, no secondary pollution and the like.

Description

[0001] The invention belongs to the technical field of soil remediation treatment; in particular, it relates to a method for repairing arsenic-contaminated soil with nano-zero-valent iron in cooperation with bacteria. technical background [0002] Arsenic (As) is a toxic element in the fifth main group of the periodic table. At present, arsenic pollution is already a global environmental problem, causing widespread concern around the world. Asia is one of the most severely polluted areas of arsenic, and groundwater is mainly polluted by arsenic. About 50 million people are affected by groundwater pollution. Long-term drinking of high-arsenic groundwater can cause diseases such as skin keratinization, skin cancer, and visceral cancer. At the same time, irrigation of farmland with high-arsenic groundwater will transfer arsenic from groundwater to the surface of the soil. In Asia, people use rice as the main food source. However, due to the flooded planting method of rice, the avai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/08
CPCB09C1/08B09C1/10
Inventor 王华伟宋宜王亚楠孙英杰
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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