Double-crosslinked gel-based micro-fluidic chip and manufacturing method thereof

A microfluidic chip and secondary cross-linking technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve the problem that the bonding interface is easy to break, the bonding process is not strong enough, and cannot be realized Bifurcated structure flow channel structure and other issues to achieve the effect of good biocompatibility, easy operation and good strength

Active Publication Date: 2019-02-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3D printing generally uses coaxial nozzles to print flow channels. This method cannot realize the structure of bifurcated flow channels; the sacrificial layer process and bonding process will introduce other substances; the bonding process is not strong, and the bonding interface is easy to break

Method used

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  • Double-crosslinked gel-based micro-fluidic chip and manufacturing method thereof
  • Double-crosslinked gel-based micro-fluidic chip and manufacturing method thereof
  • Double-crosslinked gel-based micro-fluidic chip and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Taking the fabrication of a gelatin-GelMA-based microfluidic chip with a bifurcated flow channel and the construction of a blood vessel chip as examples, the manufacturing process mentioned in the present invention is further described.

[0070] figure 1 The 3D model of the mold showing the designed bifurcated runners starts the mold manufacturing process. Existing printing materials can be selected, and corresponding 3D printing methods can be used, such as polylactic acid, photosensitive resin, etc., FDM 3D printing, photocuring printing, etc. can be used, and existing micro-manufacturing processes or traditional machining processes can also be used. , In this embodiment, a photosensitive resin is selected, and a photocuring printing method is used. After the printing is completed, the photosensitive resin mold 1 is removed from the printer, and the inner cavity of the photosensitive resin mold 1 has a mold convex groove 2 for forming a bifurcated groove structure an...

Embodiment 2

[0081] Taking the manufacture of a gelatin-sodium alginate-based microfluidic chip as an example, the manufacturing process mentioned in the present invention is further described.

[0082] Weigh a certain amount of gelatin and sodium alginate particles, and dissolve the gelatin and sodium alginate with deionized water through constant temperature magnetic stirring at 37 degrees to obtain a composite hydrogel precursor solution of 2% sodium alginate and 9% w / v gelatin , placed in a 37-degree water bath for later use.

[0083] The prepared composite hydrogel precursor solution was poured into the assembled mold.

[0084] Put it in a 4-degree refrigerator and take it out after standing for 10 minutes;

[0085] The mold is disassembled, and the partially cross-linked composite hydrogel is released from the mold to obtain a hydrogel sheet with bifurcation-shaped grooves on the surface;

[0086] The grooved surfaces of two such hydrogel sheets are closely attached, soaked in 4% c...

Embodiment 3

[0088] Taking the manufacture of a sodium alginate-GelMA-based microfluidic chip as an example, the manufacturing process mentioned in the present invention is further described.

[0089] Prepare 1% w / v (gram / ml) LAP solution with basic medium and LAP powder, weigh a certain amount of GelMA material, dissolve GelMA by vortex oscillation with LAP solution, and obtain 10% w / v (gram / ml) ) GelMA precursor solution, filtered through a 220nm filter; weigh a certain amount of sodium alginate particles, and use the GelMA precursor solution to dissolve sodium alginate through 37-degree constant temperature magnetic stirring to obtain 2% w / v (g / ml) Sodium alginate, 10% GelMA w / v (g / ml) composite hydrogel precursor solution, placed in a 37 degree water bath for use.

[0090] The prepared composite hydrogel precursor solution was poured into the assembled mold.

[0091] Soak it in 4% calcium chloride solution, let it stand for 10 minutes and take it out;

[0092] The mold is disassembled,...

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Abstract

The invention discloses a manufacturing method of a double-crosslinked gel-based micro-fluidic chip. The manufacturing method comprises the following steps: (1) carrying out three-dimensional printingor mold casting on a hydrogel precursor solution under a primary crosslinking condition to obtain two partially-crosslinked hydrogel sheets with partial channel structures; and (2) laminating the twopartially-crosslinked hydrogel sheets to abut the channel structures, and applying a secondary crosslinking condition to make the two hydrogel sheets bonded and completely crosslinked in order to obtain the double-crosslinked gel-based micro-fluidic chip. The manufacturing method of the double-crosslinked gel-based micro-fluidic chip has the advantages of simple and convenient manufacturing process, non-toxicity, harmlessness and wide application range. The gel-based micro-fluidic chip produced through the method has the advantages of good stability and reliability, various forms and good biocompatibility.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a method for manufacturing a gel-based microfluidic chip with a specific shape of internal channels based on the principle of secondary cross-linking and the prepared gel-based microfluidic chip. Background technique [0002] With the development of micro-manufacturing technology, microfluidic chip has gradually become an emerging platform for cell culture. Compared with the commonly used methods in the laboratory, the operation and analysis of cells on the microfluidic chip has many advantages: the size of the microchannel is equivalent to the size of the cell, and the perfusion culture method used by the chip is closer to the physiological state in the body, which can be more realistic. It reflects the biological characteristics of cells under physiological conditions; it can more effectively control the cell microenvironment; the chip is small in size, saving the co...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/502707B01L2200/12
Inventor 贺永聂晶高庆傅建中
Owner ZHEJIANG UNIV
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