Method for microbial degradation of total petroleum hydrocarbons

A technology for microbial degradation and total petroleum hydrocarbons, which is applied in the field of microbial degradation of total petroleum hydrocarbons, can solve the problems of high salinity and reduce the degradation rate of petroleum hydrocarbons by petroleum hydrocarbon degrading bacteria, and achieves the effect of broad application prospects.

Pending Publication Date: 2019-02-01
SOUTHWEST PETROLEUM UNIV
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  • Abstract
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Problems solved by technology

Among the currently published methods for microbial degradation of waste oil-based drilling fluid sediments, most of them are still in the initial stage of screening and breeding petroleum hydrocarbon-degrading bacteria, and the conditions for the use of selected petroleum hydrocarbon-degrading bacteria are relatively harsh, mainly in (1 ) A degrading bacteria can only have a good degradation rate at a specific temperature. (2) The selected degrading bacteria have higher requirements for salinity, and the salinity is too high or too low. The degradation rate of petroleum hydrocarbon degrading bacteria to petroleum hydrocarbons, (3) A petroleum hydrocarbon degrading bacteria can only effectively degrade a small number of components in petroleum hydrocarbons, and will not degrade all components in petroleum hydrocarbons, for example, a certain petroleum Hydrocarbon degrading bacteria can only degrade saturated hydrocarbons in petroleum, but not aromatic fen and colloid; (4) If several petroleum hydrocarbon degrading bacteria are mixed together, the degrading bacteria will inhibit each other; the temperature adapted to each degrading bacteria , Salinity resistance and PH are different, several petroleum hydrocarbon degrading bacteria mixed together have no synergistic effect

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  • Method for microbial degradation of total petroleum hydrocarbons
  • Method for microbial degradation of total petroleum hydrocarbons

Examples

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Effect test

Embodiment 1

[0026] A method for microbial degradation of total petroleum hydrocarbons, comprising the steps of:

[0027] (1) Solid-phase oil-based drilling fluid pretreatment

[0028] The bottom sediment of the waste oil-based drilling fluid is air-dried by a blower to obtain a solid phase sediment, and a pH regulator is added to the solid phase sediment to make the pH value between 6 and 8, and at the same time, the water content does not exceed 10%;

[0029] (2) Add 0.2g of SQ-1 engineering bacteria, 0.1g of SQ-2 engineering bacteria, and 1mL of SQ-3 engineering bacteria to each kilogram of solid-phase sediment in step (1), and then treat them in a warm environment at 24°C 50-60 days, during which the humidity of the solid phase deposit is maintained at 30%-40% by adding water.

Embodiment 2

[0031] A method for microbial degradation of total petroleum hydrocarbons, comprising the steps of:

[0032] (1) Solid-phase oil-based drilling fluid pretreatment

[0033] The bottom sediment of the waste oil-based drilling fluid is air-dried by a blower to obtain a solid phase sediment, and a pH regulator is added to the solid phase sediment to make the pH value between 6 and 8, and at the same time, the water content does not exceed 10%;

[0034] (2) Add SQ-1 engineering bacterium 0.2g, SQ-2 engineering bacterium 0.1g, SQ-3 engineering bacterium 1mL respectively in the solid phase deposit of every kilogram of step (1), add water to make its humidity reach 20%;

[0035] (3) Add 0.1g NH respectively in every kilogram of solid phase deposit of step (2) 4 NO 3 and 0.1 g KH 2 PO 4 , and then treated in a warm environment at 24°C for 50-60 days, during which the humidity of the solid-phase deposit was maintained at 30%-40% by adding water.

Embodiment 3

[0037] A method for microbial degradation of total petroleum hydrocarbons, comprising the steps of:

[0038] (1) Solid-phase oil-based drilling fluid pretreatment

[0039] The bottom sediment of the waste oil-based drilling fluid is air-dried by a blower to obtain a solid phase sediment, and a pH regulator is added to the solid phase sediment to make the pH value between 6 and 8, and at the same time, the water content does not exceed 10%;

[0040] (2) Add SQ-1 engineering bacterium 0.2g, SQ-2 engineering bacterium 0.1g, SQ-3 engineering bacterium 1mL respectively in the solid phase deposit of every kilogram of step (1), add water to make its humidity reach 20%;

[0041] (3) Add 0.1g NH respectively in every kilogram of solid phase deposit of step (2) 4 NO 3 and 0.1 g KH 2 PO 4, and then treated at a low temperature of 2°C for 50 to 60 days, during which the humidity of the solid phase deposit was maintained at 30% to 40% by adding water.

[0042] In Examples 1 to 3, SQ-1...

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Abstract

The invention relates to a method for microbial degradation of total petroleum hydrocarbons. The method comprises: carrying out air drying on the bottom sediment of waste oil-based drilling fluid by an air blower, adjusting the pH value to 6-8, and adjusting the water content to not more than 10%; adding 0.2-0.5 g of SQ-1 engineering bacteria, 0.1-0.4 g of SQ-2 engineering bacteria, and 1-5 ml ofSQ-3 engineering bacteria to per kg of the solid phase sediment, and adding water to achieve a humidity of 20%; and finally adding 0.1-0.4 g of NH4NO3 and 0.1-0.3 g of KH2PO4, and treating for 50-60 days in a 24-31 DEG C warm environment, wherein the humidity of the solid phase sediment is maintained at 30-40% by adding water. According to the present invention, through the synergetic effect of the three engineering bacteria, the oil-based drilling fluid petroleum hydrocarbons are rapidly degraded, and the disadvantages of harsh temperature range, poor salt tolerance of the microbial agent, less degradation components, mutual inhibition among multiple degrading bacteria and the like in the prior art are overcome.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of petroleum hydrocarbons, in particular to a method for microbial degradation of total petroleum hydrocarbons. Background technique [0002] At present, in the petroleum field, the serious harm caused by waste oil-based drilling fluid pollution has attracted more and more attention from the state. The Ministry of Environmental Protection, Ministry of Public Security and other departments of China have included petroleum hydrocarbon pollutants in the "National Hazardous Waste List". At present, the domestic treatment of waste oil-based drilling fluid sludge mainly relies on physical and chemical methods, including heat treatment, thermal distillation, solvent extraction, supercritical fluid extraction, steam stripping, pit sealing and burial, etc. These methods are mature in construction technology, but there are problems of high processing cost and unsatisfactory economic benefits i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/10
CPCC02F3/34C02F2103/10
Inventor 苏俊霖秦祖海闫璇罗亚飞王雷雯孔德光左富银李立宗赵阳曾意晴
Owner SOUTHWEST PETROLEUM UNIV
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