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Preparation method of nano-epigallocatechin gallate (EGCG)

A technology for epigallocatechin and gallate, which is applied in the field of preparation of nano-epigallocatechin gallate, can solve the problems of low bioavailability, unstable chemical properties of EGCG, etc. The effect of bioavailability, good biocompatibility

Inactive Publication Date: 2019-02-12
江苏瑾辉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Epigallocatechin gallate (EGCG for short) is a catechin monomer isolated from tea leaves. It is the main component of the biological activity of tea polyphenols. It has a variety of biological activities and has nutritional and health functions for the human body. , has antibacterial, antiviral, antioxidative, antiarteriosclerosis, antithrombotic, antiangiogenic, antiinflammatory and antitumor effects, but EGCG is chemically unstable and has low bioavailability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of nanometer epigallocatechin gallate (EGCG), comprising the following steps:

[0019] Step 1, preparation of chitosan solution A: weigh a certain mass of chitosan and add it to 1% glacial acetic acid solution, heat and stir to dissolve, configure chitosan 1.5mg / ml solution, and filter while hot to obtain solution A;

[0020] Step 2, weigh a certain amount of EGCG and add it to solution A to prepare solution B containing 0.25 mg / ml of EGCG;

[0021] Step 3, slowly add 2.5 mg / ml sodium tripolyphosphate solution C to solution B, the volume ratio of solution B and solution C is 2.5:1, and stir at high speed during the addition process to obtain nano-EGCG suspension;

[0022] In step 4, 2% mannitol is added to the nano-EGCG suspension as a freeze-drying protective agent, mixed evenly, and freeze-dried to obtain a freeze-dried product of the nano-EGCG.

Embodiment 2

[0024] A preparation method of nanometer epigallocatechin gallate (EGCG), comprising the following steps:

[0025] Step 1, preparation of chitosan solution A: weigh a certain mass of chitosan and add it to 1% glacial acetic acid solution, heat and stir to dissolve, configure chitosan 2mg / ml solution, and filter while hot to obtain solution A;

[0026] Step 2, weighing a certain amount of EGCG and adding it to solution A to prepare solution B containing 0.4 mg / ml of EGCG;

[0027] Step 3, slowly add 2.5mg / ml sodium tripolyphosphate solution C to solution B, the volume ratio of solution B and solution C is 3:1, and stir at a high speed during the addition process to obtain nano-EGCG suspension;

[0028] In step 4, 2% mannitol is added to the nano-EGCG suspension as a freeze-drying protective agent, mixed evenly, and freeze-dried to obtain a freeze-dried product of the nano-EGCG.

Embodiment 3

[0030] A preparation method of nanometer epigallocatechin gallate (EGCG), comprising the following steps:

[0031] Step 1, preparation of chitosan solution A: weigh a certain mass of chitosan and add it to 1% glacial acetic acid solution, heat and stir to dissolve, configure chitosan 2.5 mg / ml solution, and filter while hot to obtain solution A;

[0032] Step 2, weighing a certain mass of EGCG and adding it to solution A to prepare solution B containing 0.5 mg / ml of EGCG;

[0033] Step 3, slowly add 2.5 mg / ml sodium tripolyphosphate solution C to solution B, the volume ratio of solution B and solution C is 4:1, and stir at high speed during the addition process to obtain nano-EGCG suspension;

[0034] In step 4, 2% mannitol is added to the nano-EGCG suspension as a freeze-drying protective agent, mixed evenly, and freeze-dried to obtain a freeze-dried product of the nano-EGCG.

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Abstract

The invention discloses a preparation method of nano-epigallocatechin gallate (EGCG). The preparation method comprises the following steps of step one, weighing certain mass of chitosan and adding into a 1 percent glacial acetic acid solution, heating, stirring for dissolving to obtain a chitosan solution; filtering while hot to obtain a solution A; step two, weighing certain mass of EGCG and adding into the solution A to obtain a solution B; step three, slowly adding a sodium tripolyphosphate solution C into the solution B, and carrying out high-speed stirring while adding to obtain nano EGCGsuspension; step four, adding 2 percent mannitol serving as a freeze-drying protective additive into the nano EGCG suspension, uniformly mixing and carrying out freeze drying to obtain a nano EGCG lyophilized product. The EGCG nanoparticles prepared by the preparation method disclosed by invention have the characteristics of good biocompatibility, biodegradation and the like; in addition, slow release of the EGCG can be effectively restricted, the action time in vivo is prolonged and further the bioavailability of the EGCG is played more fully.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of nanometer epigallocatechin gallate (EGCG). Background technique [0002] Epigallocatechin gallate (EGCG for short) is a catechin monomer isolated from tea leaves. It is the main component of the biological activity of tea polyphenols. It has a variety of biological activities and has nutritional and health functions for the human body. , has antibacterial, antiviral, antioxidative, antiarteriosclerosis, antithrombotic, antiangioproliferative, antiinflammatory and antitumor effects, but EGCG is chemically unstable and has low bioavailability. However, chitosan nanoparticles have attracted extensive attention as new drug delivery and controlled release carriers because of their good biocompatibility, ultrafine particle size, and biodegradable properties. Therefore, the development of new products with high biological activity and bioavailability and...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K31/353A61K47/36A61P31/04A61P31/12A61P39/06A61P9/10A61P7/02A61P35/00A61P29/00
CPCA61K31/353A61K9/146A61K9/19A61K47/36A61P7/02A61P9/10A61P29/00A61P31/04A61P31/12A61P35/00A61P39/06
Inventor 蔡昌华王雷
Owner 江苏瑾辉生物科技有限公司
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