Thermo-sensitive hydrogel used for cartilage repair and preparation method and application thereof

Abstract

The invention relates to thermo-sensitive hydrogel used for cartilage repair and a preparation method and application thereof. The thermo-sensitive hydrogel used for cartilage repair is prepared froman agarose aqueous solution, a gelatin aqueous solution, a sodium hyaluronate aqueous solution and DBM-RGD particles. The preparation method of the thermo-sensitive hydrogel used for cartilage repaircomprises the steps that 1, the agarose aqueous solution is prepared; 2, the gelatin aqueous solution is prepared; 3, the sodium hyaluronate aqueous solution is prepared; 4, DBM particles are prepared; 5, the DBM-RGD particles are prepared; 6, 10 ml of the agarose aqueous solution obtained in the step 1, 10 ml of the gelatin aqueous solution obtained in the step 2, 4 ml of the sodium hyaluronate aqueous solution obtained in the step 3 and 0.24-1.2 g of the DBM-RGD particles obtained in the step 5 are taken, mixed, stirred evenly by a glass rod, and placed in a 37-DEG C constant-temperature water bath kettle after being mixed evenly to form gel, so that the thermo-sensitive hydrogel used for cartilage repair is obtained.

Description

technical field [0001] The invention belongs to the field of biomedical hydrogels, and in particular relates to a temperature-sensitive hydrogel for cartilage repair and its preparation method and application. Background technique [0002] Articular cartilage is a dense connective tissue that plays an extremely important role in buffering the stress between joints and reducing friction between articular surfaces. However, articular cartilage is easily damaged due to obesity, sports trauma or arthritis, and even a small area of ​​defect has the potential risk of serious degeneration. Moreover, the tissue structure of articular cartilage is dense, lacks nerves, blood vessels, etc., and the self-proliferation ability of chondrocytes in the articular cartilage tissue is relatively low. Therefore, once the articular cartilage is damaged, it is difficult to repair itself. [0003] The repair and regeneration of cartilage tissue defects has always been a clinical challenge that is...

AI Technical Summary

Problems solved by technology

Traditional biomedical scaffold materials such as inorganic metal materials stainless steel, aluminum alloy, etc., inorganic non-metallic materials such as bioceramics, medical carbon, etc., and organic polymer materials such as polylactic acid, polyglycolic acid, etc. have various shortcomings, such as biological Low compatibility, poor degradability, etc.

Images