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Preservative and blood collection tube for protecting nucleic acid in blood in vitro

A preservative and protective body technology, applied in the field of genetic testing, can solve the problems of short half-life, impact, and difficulty in blood preservation, and achieve the effect of inhibiting pollution

Active Publication Date: 2022-03-08
ANNOROAD GENE TECH BEIJING +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content and short half-life of free DNA in blood, coupled with factors such as weather conditions and geographical conditions, blood preservation during transportation is particularly difficult
The current commercial free nucleic acid blood collection tubes can usually only be stored for 3 to 4 days under transportation conditions, which requires strict restrictions on the transportation time of samples
If it cannot reach the testing destination within the transportation period for testing, it will lead to the degradation of free DNA or the rupture of nucleated cells, which will affect the test results with free DNA as the detection target or fail to obtain the test results

Method used

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  • Preservative and blood collection tube for protecting nucleic acid in blood in vitro
  • Preservative and blood collection tube for protecting nucleic acid in blood in vitro
  • Preservative and blood collection tube for protecting nucleic acid in blood in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The specific components of the preservative in this example are 7.5% by weight of dipotassium ethylenediaminetetraacetic acid dihydrate, 4% by weight of bis-imidazolidinyl urea, 0.79% by weight of sodium chloride, potassium dihydrogen phosphate and disodium hydrogen phosphate seven Hydrate to adjust the pH to 7.2. Follow the steps below:

[0049] 1. Use a blood collection tube added with 100 μL of the preservative of the present invention to collect venous peripheral blood, with a blood collection volume of 5 mL per tube;

[0050] 2. Pack the blood collection tubes after blood collection and carry out transportation tests on 0 days, 3 days, 7 days and 10 days by express delivery;

[0051] 3. Plasma separation after the transportation is completed. The plasma separation step is to put the blood collection tube into the centrifuge, centrifuge at 1,600g for 10 minutes, then carefully transfer the supernatant to a new centrifuge tube, centrifuge at 16,000g for 10 minutes, an...

Embodiment 2

[0055] The specific components of the preservative in this example are 9% by weight ethylenediaminetetraacetic acid dipotassium dihydrate, 10% by weight bis-imidazolidinyl urea, 0.77% by weight sodium chloride, potassium dihydrogen phosphate and disodium hydrogen phosphate seven Hydrate to adjust the pH to 7.2. Follow the steps below:

[0056] 1. Use blood collection tubes added with 100 μL of the preservative of the present invention to collect venous peripheral blood, with a blood collection volume of 5 mL per tube;

[0057] 2. Pack the blood collection tubes after blood collection and carry out transportation tests on 0 days, 3 days, 7 days and 10 days by express delivery;

[0058] 3. Plasma separation after the transportation is completed. The plasma separation step is to put the blood collection tube into the centrifuge, centrifuge at 1,600g for 10 minutes, then carefully transfer the supernatant to a new centrifuge tube, centrifuge at 16,000g for 10 minutes, and transfe...

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Abstract

The invention relates to a preservative for protecting nucleic acid in blood in vitro and a blood collection tube. Specifically, according to percentages, it includes 5-15% by weight of anticoagulant, 2-40% by weight of preservative, and 0.74-0.79% by weight of osmotic pressure regulator, and a buffer to adjust the pH value to 6.0-8.0. Using the preservative of the invention can effectively prevent cell rupture from releasing chromosomal DNA, and maintain the overall stability of free DNA in peripheral blood.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a preservative for protecting nucleic acid in blood in vitro and a blood collection tube. Background technique [0002] In recent years, with the continuous development of biomedicine and clinical diagnostic technology, more and more researches have been done on the early diagnosis, prognosis and monitoring of diseases with circulating free DNA in the blood, such as non-invasive prenatal genetic testing and tumor diagnosis Wait. [0003] Non-invasive prenatal genetic testing is the diagnosis of cell-free fetal DNA in the plasma of pregnant women. Pregnant women can detect fetal cell-free DNA from the seventh week of pregnancy. Using fetal cell-free DNA can be used for early and specific prenatal screening and diagnosis. [0004] Tumor diagnosis is the sequencing of circulating tumor DNA (ctDNA) in peripheral blood, and the detection of ctDNA can be used as a favorable t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 张介中韩典昂杜洋李艳秋玄兆伶李大为梁峻彬陈重建
Owner ANNOROAD GENE TECH BEIJING