A 3D printing tissue engineering scaffold and its preparation method
A tissue engineering scaffold and 3D printing technology, applied in 3D printing, metal processing equipment, manufacturing tools, etc., can solve problems such as difficult to load growth factors, difficult to achieve one-time molding of hydrogel materials and medical polymer materials, etc., to achieve mechanical good performance effect
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[0023] The present invention also provides a preparation method of the claimed 3D printing tissue engineering scaffold, which includes the following steps:
[0024] S1: Preparation of artificial medical polymer-hydrogel composite microspheres series with gradient content of calcium and phosphorus active biomaterials: Synthesize hydrogel microspheres containing KGN, and wrap different contents of calcium and phosphorus on the outside of the hydrogel microspheres Active biological materials and artificial medical polymer materials to obtain artificial medical polymer-hydrogel composite microspheres;
[0025] S2: Design a three-dimensional model of the porous multi-level structure through software modeling;
[0026] S3: Using the selective laser sintering rapid prototyping technology, the series of artificial medical polymer-hydrogel composite microspheres are sintered layer by layer according to the model to obtain a porous laminate with a layer-by-layer gradient change in the c...
Embodiment
[0037] S1: Preparation of a series of artificial medical polymer-hydrogel composite microspheres with gradient changes in the content of calcium and phosphorus active biomaterials. The series of artificial medical polymer-hydrogel composite microspheres refers to the artificial medical polymer-hydrogel composite microspheres corresponding to different layers in the porous laminated structure. In this embodiment, the porous laminated structure is preferably configured to include three layers, namely a shallow layer, a transition layer and a bottom layer. The artificial medical polymer-hydrogel composite microsphere series includes a shallow layer of artificial medical polymer-hydrogel composite microspheres, a transition layer of artificial medical polymer-hydrogel composite microspheres and a bottom layer of artificial medical polymer-hydrogel composite microspheres. Gel composite microspheres.
[0038] First prepare the shallow artificial medical polymer-hydrogel composite m...
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