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Lipidosome modified gold nanoparticle compound and application thereof in Parkinson's disease treatment

A technology of gold nanoparticles and complexes, applied in liposome delivery, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc.

Active Publication Date: 2019-02-15
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But so far there is no efficient and safe gene therapy drug for Parkinson's disease.

Method used

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  • Lipidosome modified gold nanoparticle compound and application thereof in Parkinson's disease treatment
  • Lipidosome modified gold nanoparticle compound and application thereof in Parkinson's disease treatment
  • Lipidosome modified gold nanoparticle compound and application thereof in Parkinson's disease treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0161] Example 1 Preparation of liposome-modified gold nanoparticle complexes

[0162] 1. Preparation of thiol-modified gold nanoparticles

[0163] Take 0.05 g of chloroauric acid solid powder and dissolve it in 50 mL of pure water, then add 5 mL of 1% sodium citrate solution and 20 μL (0.0169 g) of n-dodecyl mercaptan. Prepare sodium borohydride solution now, take 100 mL of pure water and pre-cool it in a refrigerator at 4°C, weigh 0.9 g of sodium borohydride after the temperature is stable, add to it and stir to dissolve, add 4 mL of sodium borohydride solution quickly into chloroauric acid solution while stirring It was observed that the color of the solution changed from light yellow to orange rapidly. After the color was stable, the stirring was stopped, and the sulfhydryl-modified gold nanoparticles GNP were prepared, and the GNP solution was stored in a refrigerator at 4°C.

[0164] 2. Preparation of gold nanoparticles adsorbed by pDNA

[0165] Take 900 μL of GNP sol...

Embodiment 2

[0175] Example 2 Characterization of liposome-modified gold nanoparticle complexes

[0176] 1. Ultraviolet Spectrum Detection

[0177] (1) Experimental operation

[0178] Scan the sample in the wavelength range of 190-900 nm by an ultraviolet-visible spectrophotometer to obtain the maximum absorption wavelength of the sample in this wavelength range. With dispersant water as the reference solution, 3 mL gold nanometer solution was added into the cuvette, and the spectral scanning detection was carried out in the range of 190-900 nm.

[0179] (2) Experimental results

[0180] Ultraviolet-visible spectrophotometry is the use of the absorption spectrum of substances for photometric qualitative and quantitative analysis. The absorption spectrum of a substance is essentially the result that the molecules and atoms in the substance absorb light energy of certain wavelengths in the incident light, and correspondingly, the molecular vibration energy level transition and the electro...

Embodiment 3

[0211] Example 3 The therapeutic effect of gold nanoparticle complex on PD mice

[0212] 1. Establishment of PD mouse model

[0213] (1) Experimental method

[0214] The PD mouse model was established using MPTP, and MPTP solution (30 mg / kg, twice a week, 10 times in total) was injected intraperitoneally, and the blank group was injected intraperitoneally with the same volume of normal saline at the same time every day. The expression of TH in the substantia nigra of mice was detected by immunoblotting method,

[0215] (2) Experimental results

[0216] The gold nanoparticle complex increases the expression of TH in the brain of PD mice and inhibits the overexpression of α-syn. Such as Figure 7 When detecting the expression of TH and α-syn protein in the mouse SN, we found that long-term MPTP treatment led to a significant decrease in the expression of TH in the substantia nigra, indicating that the dopaminergic nerves in the mouse brain were damaged. In addition, the expr...

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Abstract

The invention discloses a lipidosome modified gold nanoparticle compound and an application thereof in Parkinson's disease treatment. The gold nanoparticle compound is a gold nanoparticle which is wrapped with lipidosome and absorbs plasmids capable of interfering alpha-synuclein synthesis, and the surface of the gold nanoparticle compound is modified with nerve growth factors and ducosahexenoic acid. With the adoption of the prepared lipidosome modified gold nanoparticle composite material, on the premise that the problem of serious adverse effects of chemical medicines and traditional transfection reagents is solved, the plasmids capable of interfering alpha-synuclein synthesis are transfected into cells and interfere the alpha-synuclein synthesis so as to treat dyskinesia and exploration disability of a mouse with Parkinson's disease, and it is found that the lipidosome modified gold nanoparticle compound can improve athletic and exploring abilities of the mouse with Parkinson's disease by interfering expression of alpha-synuclein in the brain and has great significance and application value for treatment of the Parkinson's disease and improvement of behavior disorder.

Description

technical field [0001] The invention relates to the technical field of Parkinson's disease treatment, more specifically, to a liposome-modified gold nanoparticle complex and its application in treating Parkinson's disease. Background technique [0002] Parkinson's disease (PD), also known as Parkinson's disease, is a common neurodegenerative disease, and its incidence rate is second only to Alzheimer's disease (Alzheimer's disease, AD) Nervous disease. At present, about 1.5% of the population over the age of 65 in the world suffers from PD. By 2030, the number of PD patients may double to about 9 million. The main clinical symptoms of PD are muscle rigidity, tremor, slowed movement, and balance and walking disorders. With the deepening of PD research, researchers have found that in addition to typical movement disorders, PD patients also have many non-motor symptoms. Symptoms, such as mood disorders, anxiety, depression, and cognitive impairments such as slowed thinking an...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/62A61K47/54A61K47/26A61K33/242A61P25/16A61P25/28
CPCA61K47/26A61K47/54A61K47/62A61K47/6911A61P25/16A61P25/28A61K33/24
Inventor 关燕清李楚华刘丽李明超叶家霖王俊盈曾小蝶周素冰
Owner SOUTH CHINA NORMAL UNIVERSITY
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