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Application method of biological bacterial agent for straw biogas fermentation pretreatment

A biological bacterial agent and biogas fermentation technology, applied in the field of bioengineering, can solve the problems of inefficient biological bacterial agent and long treatment period, and achieve the effects of simple and easy-to-control application method, fewer fermentation steps, and improved hydrolysis efficiency.

Active Publication Date: 2021-07-20
河北京安瑞能环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention proposes an application method of a biological bacterial agent used for the pretreatment of straw biogas fermentation, which solves the technical problems that the existing biological bacterial agent is not efficient and the treatment period is long

Method used

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  • Application method of biological bacterial agent for straw biogas fermentation pretreatment

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Embodiment 1

[0031] A method for producing a biological bacterial agent for straw biogas fermentation pretreatment, comprising the following steps:

[0032] S1. Cultivation of seed liquid: inoculate Aspergillus niger (Aspergillus niger) suspension into 200ml seed culture medium, the inoculum size is 10mL, and cultivate in constant temperature phase for 1 day at 28°C to obtain seed liquid, seed liquid The OD600nm of the culture solution is 0.687;

[0033] The seed medium is specifically KH 2 PO 4 1.0 g / L, MgSO 4· 7H 2 O0.3 g / L, NH 4 Cl 2.0 g / L, sodium carboxymethylcellulose (CMC-Na) 5.0 g / L, NaOH adjusts the pH value to 7.0;

[0034] S2. Fermentation culture: Take 50 mL of the seed liquid obtained in S1 and inoculate it into a fermenter with an effective volume of 1 L. Before use, the fermenter is sterilized by high-temperature steam for 2 hours, cultivated in a constant temperature water bath at 28°C for 24 hours, and injects sterile air to control Dissolved oxygen (DO) is 0.1, and t...

Embodiment 2

[0047] A method for producing a biological bacterial agent for straw biogas fermentation pretreatment, comprising the following steps:

[0048] S1. Cultivation of seed liquid: inoculate the Aspergillus niger suspension into 200ml seed culture medium, the inoculum size is 40mL, cultivate in constant temperature phase for 3 days, and the cultivation temperature is 35°C to obtain seed liquid, the culture liquid OD600nm of the seed liquid is 1.115;

[0049] The seed medium is specifically KH 2 PO 4 1.0 g / L, MgSO 4· 7H 2 O0.3 g / L, NH 4 Cl 2.0 g / L, sodium carboxymethylcellulose (CMC-Na) 5.0 g / L, NaOH adjusts the pH value to 7.0;

[0050] S2. Fermentation culture: Take 200 mL of the seed liquid obtained in S1 and inoculate it into a fermenter with an effective volume of 1 L. Before use, the fermenter is sterilized by high-temperature steam for 2 hours, cultivated in a constant temperature water bath at 35°C for 30 hours, and injects sterile air to control Dissolved oxygen (DO) ...

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Abstract

The invention belongs to the technical field of bioengineering, and proposes a production method and application of a biological bacterial agent for straw biogas fermentation pretreatment, including seed liquid cultivation, fermentation cultivation, solid-liquid separation, solid drying, liquid atomization drying, mixing The preparation step is to apply the prepared biological agent to straw treatment. The treatment method includes steps such as straw crushing, biohydrolysis pretreatment and anaerobic digestion, which solves the technical problems of inefficient and long treatment period of the existing biological agent.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to an application method of a biological bacterial agent used for straw biogas fermentation pretreatment. Background technique [0002] my country is a large agricultural production country, rich in straw resources of various crops. According to incomplete statistics, the output of various crop straws reached 790 million tons in 2016, mainly including rice straw, corn straw, and wheat straw. At present, my country's straw treatment and disposal technologies mainly use direct returning to the field, incineration on the spot, or composting with livestock and poultry manure. These disposal methods not only lead to waste of resources, damage to the micro-ecological environment, damage to soil fertility and decline in soil fertility, but also seriously affect the quality of the environment and endanger human health. Pile up straw not only encroach on the land, breed mosquitoes and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P5/02C12R1/685
CPCC12N1/14C12P5/023Y02E50/30
Inventor 周思宇陈佳奇成志远梁文豹葛金龙冯远哲谢亚婵
Owner 河北京安瑞能环境科技有限公司
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