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A preparation method of schizochytrid peptide chelated calcium with antioxidant activity

A technology of anti-oxidative activity and anti-oxidative peptides, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., to achieve the effects of simple preparation process, improved resource utilization rate, and product safety

Active Publication Date: 2022-02-11
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the preparation of Schizochytrium peptide chelated calcium with antioxidant activity using the remaining algae residue after oil extraction from Schizochytrium sp.

Method used

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  • A preparation method of schizochytrid peptide chelated calcium with antioxidant activity
  • A preparation method of schizochytrid peptide chelated calcium with antioxidant activity
  • A preparation method of schizochytrid peptide chelated calcium with antioxidant activity

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Effect test

Embodiment 1

[0040] A preparation method of schizochytrid peptide chelated calcium with antioxidant activity, comprising the following steps:

[0041] (1) Use the remaining algae residue after extracting oil from Schizochytrium algae as raw material, pulverize it with a pulverizer, pass through a 120-mesh sieve, add water according to the weight ratio of material to water of 1:8, adjust the water temperature to 50°C, and add cellulase and starch at the same time For the enzyme, the mass ratio of the two compounded is 2:1, the total mass added by the two is 2% of the mass of the Schizochytrium slag powder, the pH is adjusted to 6.0, and the hydrolysis time is 1.5h. Ultrasonic treatment is assisted in the hydrolysis process, the ultrasonic treatment frequency is 20KHz, the ultrasonic power is 300W, and the ultrasonic action time is 0.75h. After the hydrolysis is completed, centrifuge at 8000r / min for 30min with a rotating speed, and take the centrifuged sediment;

[0042] (2) Take the centr...

Embodiment 2

[0047] A preparation method of schizochytrid peptide chelated calcium with antioxidant activity, comprising the following steps:

[0048] (1) Use the remaining algae residue after extracting oil from Schizochytrium algae as raw material, pulverize it with a pulverizer, pass through a 150-mesh sieve, add water according to the weight ratio of material and water of 1:12, adjust the water temperature to 60°C, and add cellulase and starch at the same time The mass ratio of the two enzymes is 1:1, the total mass of the two added is 1.5% of the mass of the Schizochytrium slag powder, the pH is adjusted to 6.5, and the hydrolysis time is 2h. Ultrasonic treatment is assisted in the hydrolysis process, the ultrasonic treatment frequency is 30KHz, the ultrasonic power is 250W, and the ultrasonic action time is 1h. After the hydrolysis is completed, centrifuge at 10000r / min for 20min with a rotating speed, and take the centrifuged sediment;

[0049](2) Take the centrifuged sediment, add...

Embodiment 3

[0054] A preparation method of schizochytrid peptide chelated calcium with antioxidant activity, comprising the following steps:

[0055] (1) Use the remaining algae residue after extracting oil from Schizochytrium algae as raw material, pulverize it with a pulverizer, pass through an 80-mesh sieve, add water according to the weight ratio of material and water of 1:5, adjust the water temperature to 55°C, and add cellulase and starch at the same time The mass ratio of the two enzymes is 1:2, the total mass of the two added is 3% of the mass of the Schizochytrium slag powder, the temperature is adjusted to 55°C, the pH is adjusted to 6.5, and the hydrolysis time is 2.5h. Ultrasonic treatment is assisted in the hydrolysis process, the ultrasonic treatment frequency is 40KHz, the ultrasonic power is 400W, and the ultrasonic action time is 1h. After the hydrolysis is completed, centrifuge at 10000r / min for 30min with a rotating speed, and take the centrifuged sediment;

[0056] (...

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Abstract

The present invention provides a preparation method of Schizochytrium peptide chelated calcium with antioxidant activity, comprising the following steps: (1) ultrasonic-assisted cellulase and amylase hydrolysis; (2) compound hydrolysis of three kinds of proteases; ( 3) Separation by ultrafiltration; (4) Ultrasound-assisted chelation of peptides and calcium ions to prepare Schizochytrium peptide chelated calcium with antioxidant activity. The preparation method of the Schizochytrium peptide chelated calcium with antioxidant activity is simple in process and can be applied in large-scale actual production. The remaining algae residue after oil extraction from Schizochytrium is used as raw material, which improves the resource utilization of Schizochytrium rate; the schizochytrid peptide chelated calcium prepared by the method of the present invention has dual biological activities of anti-oxidation and promotion of calcium ion absorption.

Description

technical field [0001] The invention belongs to the technical field of functional peptide chelated calcium ions, and specifically relates to a preparation method of chelated calcium with antioxidative activity of schizochytrium peptide, and also relates to the preparation method of schizochytrium peptide with antioxidative activity Chelated Calcium. Background technique [0002] Peptide-mineral ion chelate is a metal-organic compound, which is prepared by chelation reaction between mineral ions and peptides. It can improve the bioavailability of metal ions by virtue of the absorption mechanism of peptides in the body. Physiological and biochemical properties that inorganic metal ions do not have. At present, the chelate prepared by chelating essential trace elements and peptides has become a new type of mineral ion supplement, and has attracted more and more attention. [0003] At the same time, because peptides with specific molecular weight and amino acid sequence also h...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/107
CPCC12P21/06C07K1/107C07K1/34
Inventor 胡晓李来好杨贤庆陈胜军吴燕燕郝淑贤林婉玲马海霞
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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