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Construction method and detection method of HPLC feature map of golden cypress medicinal material

A technology of characteristic map and construction method, which is applied in the field of construction of HPLC characteristic map of Phellodendron phellodendron, can solve the problems that berberine and phellodendron can not fully reflect the quality, and cannot fully reflect the material characteristics of traditional Chinese medicine decoction, so as to improve quality control Level, good separation, good reproducibility

Active Publication Date: 2019-02-22
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The 2015 edition of "Chinese Pharmacopoeia" uses berberine and phellodendron as the quality evaluation indicators of phellodendron, and only controlling the content of berberine and phellodendron cannot fully reflect its quality
Currently, most of the fingerprints of Phellodendron phellodendrons reported in the literature use the conventional HPLC method, and all of them only focus on the material basis of the original medicinal materials. Most of the index components are fat-soluble components as the research objects, which cannot fully reflect the material basis characteristics of traditional Chinese medicine decoctions.

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  • Construction method and detection method of HPLC feature map of golden cypress medicinal material
  • Construction method and detection method of HPLC feature map of golden cypress medicinal material
  • Construction method and detection method of HPLC feature map of golden cypress medicinal material

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Embodiment 1

[0069] Embodiment 1, the construction method of the HPLC feature collection of Phellodendron Phellodendri medicinal materials

[0070] The present embodiment provides a method for building the HPLC characteristic spectrum of Cortex Phellodendri medicinal material, comprising the steps of:

[0071] (1) Preparation of reference solution

[0072] Take 0.1g of Cortex Phellodendri reference medicinal material, put it in a stoppered Erlenmeyer flask, add 50ml of 50% (v / v) methanol solution, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let cool, shake well, filter, and take The filtrate was used as the reference substance solution of the contrast medicinal material.

[0073] Another appropriate amount of berberine hydrochloride reference substance and phellodendron hydrochloride reference substance were taken, accurately weighed, and methanol was added to make a mixed solution containing 0.1 mg per 1 ml, as the reference substance reference solution.

[0074] (2) p...

Embodiment 2

[0087] Embodiment 2, the determination of the HPLC characteristic map construction condition of Cortex Phellodendri medicinal material

[0088] 1 feature map

[0089] Phellodendron Phellodendri is the dry bark of Phellodendron Phellodendron, a plant of the Rutaceae Phellodendron genus, which is the base plant of traditional Chinese medicine Phellodendron Phellodendri. It mainly contains a variety of alkaloids such as berberine, magnolanine, phellodendron, palmitate and other alkaloids, as well as lactones, sterols, mucus, etc. Berberine alkaloids are the main active ingredients in Cortex Phellodendri, which have antibacterial effect, and Cortex Cortex is the characteristic component in Cortex Phellodendri. After research, HPLC was used to establish the fingerprint of Cortex Phellodendri, which provided an effective method for the quality control of Cortex Phellodendri.

[0090] 1.1 Instruments, reagents and reagents

[0091] Instruments: Waters high performance liquid chr...

Embodiment 3

[0239] Embodiment 3, the construction method of the HPLC feature collection of Phellodendron Phellodendri medicinal materials

[0240] The present embodiment provides a kind of detection method of Cortex Phellodendri medicinal material, and the steps are as follows:

[0241] (1) Preparation of reference solution

[0242] Take 0.1g of Cortex Phellodendri reference medicinal material, put it in a stoppered Erlenmeyer flask, add 50% methanol 50ml, ultrasonically treat (power 250W, frequency 40kHz) for 30 minutes, let cool, shake well, filter, take the filtrate as the reference medicinal material substance solution. Another appropriate amount of berberine hydrochloride reference substance and phellodendron hydrochloride reference substance were taken, accurately weighed, and methanol was added to make a mixed solution containing 0.1 mg per 1 ml, as the reference substance reference solution.

[0243] (2) preparation of need testing solution

[0244] Get 0.1g of the test produ...

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Abstract

The invention relates to a construction method and a detection method of a HPLC feature map of a golden cypress medicinal material. The construction method comprises the following steps: reference solution preparation: preparing a golden cypress control medicinal material reference solution and a berberine hydrochloride and phellodendrine hydrochloride reference substance solution; sample solutionpreparation: preparing a golden cypress medicinal material sample solution and a decoction sample solution; high-performance liquid chromatography test: absorbing the control medicinal material reference solution, the reference substance solution, the medicinal material sample solution and the decoction sample solution; injecting into a liquid chromatograph, comparing a medicinal material samplemap with a decoction sample map, determining water soluble common peaks and obtaining the UPLC feature map of the golden cypress medicinal material. The HPLC feature map obtained by the construction method not only can qualitatively and quantitatively analyze the quality of the golden cypress medicinal material, but also can reflect the quality of golden cypress standard decoction as a reference.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a construction method and a detection method of an HPLC characteristic map of Cortex Phellodendri. Background technique [0002] Phellodendron chinense is the dry bark of Rutaceae Phellodendron chinense Schneid. It has the effects of clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores, and is used for damp-heat diarrhea, jaundice, red urine, vaginal itching, hot stranguria, astringent pain, bone steaming, labor heat, night sweats, sore swelling, eczema Diseases such as eczema. The main active ingredients of Cortex Phellodendri are alkaloids, including berberine, phellodendron, jatrorrhizine, and palmatine. Among them, berberine and phellodendron have pharmacological effects such as anti-inflammatory, antibacterial, antipyretic and analgesic, and are the main ones. Pharmacologically active ingredients. It has a strong cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36
CPCG01N30/02G01N30/06G01N30/36
Inventor 霍文杰王闽予魏梅程学仁黄小丹潘礼业陈万发陈向东
Owner GUANGDONG YIFANG PHARMA
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