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Subshrubby peony bark tissue culture and rapid propagation method

A technology for tissue culture rapid propagation and Paeonia suffruticosa, applied in the field of plant tissue culture, can solve the problems of restricting rapid propagation and breeding of new varieties, low reproduction coefficient, long growth cycle, etc. The effect of protecting wild resources and fast reproduction

Inactive Publication Date: 2019-03-01
张世燊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional breeding methods of Moutan bark have problems such as low reproduction coefficient, long growth cycle, and species degradation, which limit the work of rapid breeding and new species selection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction culture: After the collected stems are washed with clear water to remove surface attachments, soak in laundry detergent for 7 minutes, then rinse with running water for 2 hours, disinfect with 75% ethanol solution in an ultra-clean workbench for 12 seconds, and then wash with sterile water 5 times, and then sterilized with 0.1% mercuric chloride solution for 12 minutes, rinsed with sterile water for 5 times, sucked out the water with sterile filter paper, and inoculated into the induction medium for bud induction culture. After inoculation, first cultivate in total darkness at 26°C for 8 days, then place it under light for 13 hours a day, with a light intensity of 2200 lx, and place it under the condition of 26°C for 26 days to differentiate adventitious buds, and after 40 days Adventitious buds were produced, and the induction rate was 85%. The induction medium is: MS+1.2mg / L 6-BA+0.5mg / L NAA+22g / L sucrose+4.2g / L agar, with a pH of 5.2.

[0013] (2) Prol...

Embodiment 2

[0017] (1) Induction culture: After the collected stems are washed with clear water to remove surface attachments, soak in laundry detergent for 9 minutes, then rinse with running water for 4 hours, disinfect with 75% ethanol solution in an ultra-clean workbench for 32 seconds, and then wash with sterile water 6 times, and then sterilized with 0.1% mercuric chloride solution for 17 minutes, rinsed with sterile water for 7 times, sucked out the water with sterile filter paper, and inoculated into the induction medium for bud induction culture. After inoculation, culture in total darkness at 30°C for 9 days, then place in light for 15 hours a day with a light intensity of 3100lx, and culture at a temperature of 30°C for 22 days to differentiate adventitious buds. After 39 days, the Adventitious buds were produced, and the induction rate was 88.0%. The induction medium is: MS+3.2mg / L 6-BA+0.6mg / L NAA+27g / L sucrose+4.8g / L agar, with a pH of 5.2.

[0018] (2) Proliferation culture...

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Abstract

The invention discloses a subshrubby peony bark tissue culture and rapid propagation method, and solves the problems that artificial cultivation varieties are mixed and subjected to vegetative propagation for a long time, so that varieties are degenerated, diseases are severe, yield is reduced and the like. Plant tissue culture has the advantages of rapid propagation, low cost, high multiplicationcoefficient and the like. According to the method, stems with buds serve as an explant, a subshrubby peony bark tissue culture and rapid propagation system is built through steps such as induction, propagation, rooting, seedling exercising, transplantation and the like, the propagation speed of seedlings can be improved, the propagation scale of the seedlings can be enlarged, high-yield and excellent quality new varieties can be bred based on the propagation speed and the propagation scale, economic benefits of subshrubby peony bark planting are increased, the practical problem of demand of subshrubby peony barks is relieved, and the method has great significance for protection of wild resources.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Cortex Moutan. Background technique [0002] Cortex Moutan is a plant of Ranunculaceae, the dry root bark of peony. Dig out the roots in autumn, remove fibrous roots and stem bases, cut the bark straight with a knife, remove the xylem, and dry the root bark in the sun. Dried in the sun, it is called Gua Danpi or Fen Danpi. Gas aroma, slightly bitter taste. Has heat-clearing and cooling blood, promoting blood circulation and removing blood stasis. It is used for the treatment of febrile disease with heat-toxin spots, vomiting blood, night heat and morning cool in the late stage of febrile disease, amenorrhea, dysmenorrhea, etc. The traditional propagation method of Moutan bark has problems such as low reproduction coefficient, long growth cycle, and species degradation, which limit the work o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张世燊
Owner 张世燊
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