Preparation method of furfuryl alcohol

A technology of furfuryl alcohol and furfural, applied in the fields of biocatalysis and biomanufacturing, can solve the problems of restricting economy and practicability, increasing the difficulty of separation and purification of products, etc. Effect

Inactive Publication Date: 2019-03-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the recombinant strain E.coli CCZU-K14 can catalyze the conversion of 200mM furfural into furfuryl alcohol with high efficiency and high selectivity, the biotransformation needs to add an excess of co-substrate (300mM glucose) and coenzyme pre-substrate (400mM xylose). This not only greatly limits the economy and practicability of the process, but also increases the difficulty of subsequent product separation and purification (Bioresour.Technol.2017, 238, 698)

Method used

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  • Preparation method of furfuryl alcohol
  • Preparation method of furfuryl alcohol
  • Preparation method of furfuryl alcohol

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Acclimatization and activation of Pichia pastoris SC1103: Pichia mongolia SC1103 strains were inserted into yeast extract powder peptone glucose (YPD) liquid medium containing 5mM HMF (1% yeast extract, 2% peptone and 2% glucose ), after culturing at 30°C and 200r / min for 12h, the above-mentioned bacterial suspension was introduced into fresh YPD liquid medium containing 5mM HMF with an inoculum size of 2%, and cultivated at 30°C and 200r / min for 12h. Subsequently, the above-mentioned bacterial suspension was successively inserted into yeast extract powder peptone glucose liquid medium containing 10 and 15 mM 5-hydroxymethylfurfural with an inoculation amount of 2%, and similar steps were adopted for acclimation respectively. Put the above-mentioned acclimatized Pichia mongolica SC1103 strain into YPD liquid medium, and after culturing at 30°C and 200r / min for 12h, add the above-mentioned bacterial suspension into fresh YPD liquid with an inoculum amount of 2%. In the c...

Embodiment 2

[0030] Immobilization of Pichia mongolica SC1103: Weigh 2.5g of sodium alginate into a Erlenmeyer flask, add 100mL of distilled water, and heat to dissolve. After the solution was cooled to room temperature, the cells acclimated and activated in Example 1 were added at a concentration of 200 mg / mL-600 mg / mL (based on the wet weight of the cells). Add 0.2M CaCl drop by drop with a sterile syringe 2 After standing in the solution for 4 hours to solidify, the solution was washed with Tris-HCl buffer (100 mM, pH 8) for 3 times, and stored in a refrigerator at 4°C for use.

Embodiment 3

[0032] Add furfural and glucose to 4mL acetate buffer (50mM, pH 5) and mix evenly. The final concentrations of furfural and glucose are 50mM and 30mM respectively, and then add the concentration of 30mg (wet cell weight) / mL (buffer solution) to the embodiment 2 The prepared immobilized Pichia mongolica SC1103 cells were reacted at 35° C. and 200 r / min. The reaction was monitored by liquid chromatography ( image 3 ). After 8 hours, the yield of furfuryl alcohol was 90%, and the selectivity was 98%.

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Abstract

The invention belongs to the fields of biological catalysis and bio-manufacturing, and discloses a preparation method of furfuryl alcohol. Immobilizedmeyerozymaguilliermondii SC1103 is used for catalyzing reduction of furfuraldehyde to prepare the furfuryl alcohol. The preparation method of the furfuryl alcohol has the advantages of high selectivity, mild reaction condition, environmental friendliness, high reaction speed, high spatiotemporal yield of a target product, easy recovery of a catalyst, and the like.

Description

technical field [0001] The invention belongs to the field of biocatalysis and biomanufacturing, and in particular relates to a method for preparing furfuryl alcohol by using immobilized yeast cells to catalyze the selective reduction of furfural. Background technique [0002] Furfural is an important bio-based platform compound (Green Chem. 2010, 12, 539), which is mainly prepared from agricultural wastes such as food crop residues or wood wastes. Currently, the global annual output of furfural is about 280,000 tons (Energy Environ. Sci. 2016, 9, 1144). Among them, my country is the main producer of furfural, and 70% of the world's furfural is produced by my country. Furfural molecules contain aldehyde groups and aromatic heterocycles, which can be further transformed into various high value-added chemicals. Furfuryl alcohol is a selective reduction product of aldehyde group in furfural molecule, which has important application value in the fields of medicine, polymer, foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04C12R1/645
CPCC12P17/04
Inventor 李宁宗敏华徐中华
Owner SOUTH CHINA UNIV OF TECH
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