Unlock instant, AI-driven research and patent intelligence for your innovation.

Kit for detecting ADV (respiratory adenovirus), SP (Streptococcus pneumoniae) and BP (Bordetella pertussis) simultaneously and use method of kit

A technology of Streptococcus pneumoniae and detection reagent, which is applied in the field of molecular biology to achieve the effect of ensuring timeliness, strong specificity, rapid and accurate detection

Pending Publication Date: 2019-03-01
NANJING LANSION BIOTECH CO LTD
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no registered similar products on the market for the detection of respiratory adenovirus, Streptococcus pneumoniae and pertussis bacillus by fluorescent quantitative PCR method, and there are very few single detection products for these three pathogens, and all of them are immunoassays

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting ADV (respiratory adenovirus), SP (Streptococcus pneumoniae) and BP (Bordetella pertussis) simultaneously and use method of kit
  • Kit for detecting ADV (respiratory adenovirus), SP (Streptococcus pneumoniae) and BP (Bordetella pertussis) simultaneously and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: The kit for detecting respiratory adenovirus, Streptococcus pneumoniae and Bacillus pertussis in this embodiment consists of a detection tube containing a detection reagent, a positive quality control and RNase-free water; wherein, the detection reagent is divided into a single tube Packed in dry powder form, including primers and TaqMan fluorescent probes with specific conserved sequences of respiratory adenovirus, Streptococcus pneumoniae and Bacillus pertussis;

[0067] The primer sequence of the specific conserved sequence is:

[0068] The primer sequence of the specific conserved sequence is:

[0069] Respiratory adenovirus, SEQ ID NO.1 and SEQ ID NO.2;

[0070] Streptococcus pneumoniae, SEQ ID NO.4 and SEQ ID NO.5;

[0071] Bacillus pertussis, SEQ ID NO.7 and SEQ ID NO.8;

[0072] The TaqMan probe sequence of the specific conserved sequence is:

[0073] Respiratory adenovirus, SEQ ID NO.3;

[0074] Streptococcus pneumoniae, SEQ ID NO.6;

[0075...

Embodiment 2

[0101] Use the kit of the present invention to detect respectively the plasmid samples corresponding to respiratory adenovirus, Streptococcus pneumoniae and B. pertussis, and the mixed plasmid samples of the three, with concentrations of 10 5 copies / μL. Detection was carried out in the same manner as in Example 1.

[0102] The detection results show that the kit of the present invention can simultaneously detect three kinds of pathogens in one detection tube, and the three fluorescent signal channels do not interfere with each other, the results are shown in figure 2 . Among them, 1 is the fluorescence threshold, 2 is the negative control, 3 is the mixed plasmid of the three, 4 is the sample of respiratory adenovirus, 5 is the sample of Streptococcus pneumoniae, and 6 is the sample of B. pertussis. Negative control 2 had no amplification curve, and mixed plasmid sample 3 detected three amplification curves, which were three channels, and the Ct value was <35, indicating tha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting ADV (respiratory adenovirus), SP (Streptococcus pneumoniae) and BP (Bordetella pertussis) simultaneously. The kit comprises detection tubes containing detection reagents, as well as a positive quality control material and Rnase-free water, wherein the detection reagents are charged in different tubes, are in a form of dry powder and comprise primers andTaqMan fluorescent probes with specific conserved sequences of ADV, SP and BP. The kit can detect the ADV, SP and BP rapidly and accurately and can be simple and easy to use in various environments, and timeliness, specificity and sensitivity of detection are guaranteed; the ADV, SP and BP can be detected simultaneously and distinguished by designing the specific primers and probes on the respective conserved sequences of the ADV, SP and BP; the problems of low-temperature preservation and troublesome use and operation are solved, the detection reagents can be preserved at the low temperatureof 4 DEG C or normal temperature, and all that is needed is to add extracted nucleic acid samples during use for device detection.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a kit for simultaneously detecting respiratory adenovirus, Streptococcus pneumoniae and Bacillus pertussis and a use method thereof. Background technique [0002] Respiratory Adenovirus (ADV), Streptococcus pneumoniae (SP) and Bordetella pertussis (Bordetella pertussis, BP) are common pathogens that cause respiratory diseases in children and pose a great threat to the health of infants and young children. ADV, SP and BP infection has become a global problem. Molecular diagnostic technology can meet the needs of rapid diagnosis of ADV, SP and BP, and because their genetic material is deoxyribonucleic acid, no reverse transcription process is required after sample extraction, and molecular detection can be carried out directly, so it can be quickly and accurately Carry out pathogen screening, so as to cooperate with correct treatment, reduce the misuse of antibacterial drugs. [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/689C12Q1/686C12Q1/04C12R1/46C12R1/01
CPCC12Q1/686C12Q1/689C12Q1/701C12Q2563/107C12Q2561/101
Inventor 许行尚杰弗瑞·陈王龙于沛张蓉蓉
Owner NANJING LANSION BIOTECH CO LTD