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Application of NOC4L protein as nonalcoholic fatty liver biomarker and therapeutic target

A biomarker and non-alcoholic technology, applied in the field of biomarkers, can solve the problems of lack of effective drugs and treatment methods, and achieve the effect of inhibiting the accumulation of lipid droplets, reducing the accumulation of lipid droplets, and reducing body fat percentage

Inactive Publication Date: 2019-03-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is still a lack of effective drugs and treatments for this problem

Method used

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  • Application of NOC4L protein as nonalcoholic fatty liver biomarker and therapeutic target
  • Application of NOC4L protein as nonalcoholic fatty liver biomarker and therapeutic target
  • Application of NOC4L protein as nonalcoholic fatty liver biomarker and therapeutic target

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The inventors previously selected 6-week-old C57 / BL6 mice and fed them with normal diet (CD) and high-fat diet (HFD, 60% kal, D12492, Research Diets Inc) for 14 weeks respectively, and recorded the weight changes of the mice every week. The expression of NOC4L in the liver of CD-induced mice and HFD-induced obese mice was detected at RNA level and protein level, and it was found that the expression of NOC4L in the liver of mice fed with common diet was higher than that of mice fed with high-fat diet ( figure 1 ).

Embodiment 2

[0029] The Noc4l CDS was inserted into the PLV-EGFP-2A-puro plasmid (purchased from Yingmao Shengye Company, catalog number Cat.No.VL3402). The sequencing results were verified to be correct. HEK293T cells were co-transfected with PLV-EGFP-2A-puro-Noc4l plasmid, psPAX2 and Pmd2.G (both second-generation lentiviral packaging plasmids, both purchased from Miaoling plasmid platform), and the virus was collected at 48-72h, and 0.45 Filter through a μm filter to remove cell debris. PLV-EGFP-2A-puro (abbreviated LV-null) and PLV-EGFP-2A-puro-Noc4l (abbreviated LV-Noc4l) virus concentration was diluted to 10 9 TU / ml, injected into mice by tail vein injection. After 3 weeks, the mice were fasted overnight, and the fasting blood glucose was detected, namely the glucose tolerance test (GTT). The mice were fasted for 16 hours, and 2 g of glucose was injected intraperitoneally per kilogram of body weight. At 0, 15, 30, 60, and 120 minutes, blood was collected from the tail vein to det...

Embodiment 3

[0031] I.V. Injection of LV-null and LV-Noc4l in HFD-induced obese mice for body fat analysis, with the help of instrument (MesoQMR23-060H-I of Shanghai Numai Electronic Technology Co., Ltd.) for detection, the results found that I.V. Injection of LV-Noc4l group of mice significantly reduced body fat and increased muscle mass ( image 3 ).

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Abstract

The invention relates to biomarkers, and particularly discloses application of NOC4L protein as a nonalcoholic fatty liver biomarker and a therapeutic target. Researches find that the expression levelof the NOC4L protein is related to nonalcoholic fatty liver, and also find that the NOC4L protein can inhibit the accumulation of lipid droplets in the liver. Over expression of the NOC4L in obese mice induced by high-fat diet results in a significant decrease in lipid droplet accumulation in fatty liver accompanied by a decrease in body fat percentage. The application of the NOC4L protein as thenonalcoholic fatty liver biomarker and the therapeutic target provides a new target for the study of novel drugs for the treatment of nonalcoholic fatty liver.

Description

technical field [0001] The present invention relates to biomarkers, in particular to the application of NOC4L protein as nonalcoholic fatty liver biomarker and therapeutic target. Background technique [0002] With the continuous improvement of people's material living standards, coupled with irregular life and poor eating habits, obesity has become a major cause of affecting the quality of life. The hazards of obesity can be roughly divided into 8 types: dyslipidemia, increased cerebrovascular disease, increased probability of high blood pressure, increased cardiac load, fatty liver, increased risk of diabetes, bone and joint disease, and susceptibility to cancer. [0003] Non-alcoholic fatty liver disease (NAFLD) refers to the diffuse fatty infiltration of the liver caused by factors other than alcohol. With the improvement of living standards, the incidence of NAFLD continues to increase. The incidence rate of obesity is as high as 20%-33%, while the incidence rate of ob...

Claims

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Application Information

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IPC IPC(8): G01N33/68C12N15/867A61K45/00A61P1/16
CPCG01N33/68A61K45/00A61P1/16C12N15/86C12N2740/15043G01N2800/085
Inventor 李向东贾力娜任新敏
Owner CHINA AGRI UNIV
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