High-efficiency mutation breeding method of red beech leaf color mutant

Abstract

The invention relates to a mutation breeding method of a forest tree leaf color mutant, and discloses a high-efficiency mutation breeding method of a red beech leaf color mutant. The high-efficiency mutation breeding method mainly comprises the following steps that (1) full seeds of red beech after air separation are stored and germinated in wet sand at 2-6 DEG C; (2) when 15-30% of the seeds arerevealed, a mixed solution of 0.5mol / L calcium chloride and 0.02mol / L ethylmethane sulfonate is used for soaking the seeds for 60-80 min; (3) the seeds are rinsed with water for 3-5 times and then sown, management is performed according to a conventional method, after one month, branches with leaf color mutation gradually appear, mutant strains can reach about 0.28%, and the leaf color mutant strains are marked; (4) at the beginning of March of a following year, the branches with stable leaf color mutation are selected and grafted on red beech rootstocks which are 1-2 years old and 0.8-1.3cm in ground diameter, and routine management is performed after grafting; and (5) buds on the leaf color mutation branches are taken for further grafting and expanding propagation, and new red beech leafcolor seedlings are cultivated.

Description

Technical field: [0001] The invention relates to a method for mutation breeding of new varieties of forest tree leaf color, in particular to a method for efficient mutation breeding of red beech leaf color mutants. Background technique: [0002] Red beech is distributed intermittently from East Asia to the Mediterranean region, and the middle and lower reaches of the Yangtze River in my country is the central area of ​​red beech distribution. The red beech tree is tall and beautiful, with reddish to deep red leaves when mature. It is a tree species integrating timber, landscape and ecological functions. Beech wood is hard, with clear texture and layer upon layer. It is called "pagoda pattern" by craftsmen. It is a precious material for interior decoration and high-end furniture. [0003] Domestic research on red beech mainly focuses on cultivation, seed selection, tissue culture and biological characteristics. Zhang Lijun (1999), Zhou Wenming (2001) and others have studied...

AI Technical Summary

Problems solved by technology

At present, in terms of genetic improvement of beech beech, most studies are limited to the variation of seedling traits, and there is no report on the high-efficiency mutation breeding of beech leaf color.