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Preparation method for stepwise and high-efficiency preparation of antioxidation peptide of internal organs of sea cucumbers

A technology of sea cucumber viscera and antioxidant peptides, applied in the field of polypeptides, can solve the problems of not improving the extraction rate of sea cucumber visceral antioxidant peptides, wasting macromolecular proteins, etc., and achieve the effect of increasing the yield and improving the efficiency of enzymatic hydrolysis

Inactive Publication Date: 2019-03-12
SHENGTIAN FUQING FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is applied to the extraction of antioxidant peptides from sea cucumber viscera, the extraction rate of antioxidant peptides from sea cucumber viscera will not be improved. On the contrary, if ultra-high pressure combined with micro-fluidics is used to directly pretreat sea cucumber viscera, only the high-level structure of macromolecules in sea cucumber viscera will be extracted. The protein is decomposed into a primary protein structure that is easier to decompose. Compared with the patent application number CN104402972A, the extraction rate can be improved, but in the separation of the enzymatic solution, too much waste (based on the cost of the enzyme) is unavoidable. macromolecular protein

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  • Preparation method for stepwise and high-efficiency preparation of antioxidation peptide of internal organs of sea cucumbers
  • Preparation method for stepwise and high-efficiency preparation of antioxidation peptide of internal organs of sea cucumbers
  • Preparation method for stepwise and high-efficiency preparation of antioxidation peptide of internal organs of sea cucumbers

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preparation example Construction

[0045] A step-by-step and efficient preparation method for preparing sea cucumber visceral antioxidant peptides, comprising the following steps:

[0046] S1: Preparation of sea cucumber homogenate: separate and remove gonads, gallbladder and other organs from the internal organs of sea cucumber, wash them with water for 2 to 4 times, grind them into a slurry, mix the slurry with isopropanol at a ratio of 1:4 (w / v, (i.e. mass concentration) proportionate mixing, degreasing 2 to 4 times, standing still until the isopropanol volatilizes completely, adding deionized water 2 to 4 times the volume of the slurry, and boiling water bath for 8 to 12 minutes to inactivate endogenous enzymes, Freezing and drying to obtain dried sea cucumber powder; taking the dried sea cucumber powder and dissolving it in deionized water to obtain sea cucumber homogenate, wherein the dry powder and deionized water are in a weight ratio of 1:2 to 1:8;

[0047] S2: Ultrasonic-assisted enzymatic hydrolysis:...

Embodiment 1

[0056] Example 1: A step-by-step and efficient preparation method for preparing sea cucumber visceral antioxidant peptides, comprising the following steps:

[0057] S1: Preparation of sea cucumber homogenate: separate and remove the gonads, gallbladder and other organs from the internal organs of sea cucumber, wash them with water for 4 times, and grind them into a slurry. (mass concentration) was mixed in proportion, degreased 3 times, left to stand until the isopropanol volatilized completely, then added deionized water 2 times the volume of the slurry, in a boiling water bath for 12 minutes to inactivate endogenous enzymes, freeze-dried to obtain dried sea cucumber powder The sea cucumber homogenate obtained by dissolving sea cucumber dry powder in deionized water, wherein the dry powder and deionized water is 1:5 by weight;

[0058] S2: Ultrasonic-assisted enzymatic hydrolysis: preheat the sea cucumber homogenate prepared in S1 at a temperature of 50°C for 15 minutes, add ...

Embodiment 2

[0066] A step-by-step and efficient preparation method for preparing sea cucumber visceral antioxidant peptides, comprising the following steps:

[0067]S1: Preparation of sea cucumber homogenate: separate and remove gonads, gallbladder and other organs from sea cucumber viscera, wash them twice with clean water, grind them into a slurry, mix sea cucumber viscera slurry with isopropanol at a ratio of 1:4 (w / v is (mass concentration) was mixed in proportion, degreased 4 times, left to stand until the isopropanol volatilized completely, then added deionized water 3 times the volume of the slurry, in a boiling water bath for 8 minutes to inactivate endogenous enzymes, freeze-dried to obtain dried sea cucumber powder The sea cucumber homogenate obtained by dissolving sea cucumber dry powder in deionized water, wherein the dry powder and deionized water is 1:8 by weight;

[0068] S2: Ultrasonic-assisted enzymatic hydrolysis: preheat the sea cucumber homogenate prepared in S1 at a t...

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Abstract

The invention relates to the field of polypeptide and particularly relates to a preparation method for stepwise and high-efficiency preparation of antioxidation peptide of internal organs of sea cucumbers. The preparation method comprises the specific steps of: (S1) pretreating the internal organs of the sea cucumbers into sea-cucumber homogenate; (S2) adding protease into the sea-cucumber homogenate in step (S1) to carry out primary enzymolysis, centrifuging and separating after enzymolysis to obtain precipitate and supernatant, and separating the supernatant into a first micromolecular polypeptide solution and a macromolecular polypeptide solution by a membrane; (S3) after mixing the precipitate and the macromolecular polypeptide solution obtained in step (S2), adding protease for secondary enzymolysis, centrifuging and collecting the supernatant after enzymolysis, and separating out a second micromolecular polypeptide solution by a membrane; (S4) drying the first micromolecular polypeptide solution and the second micromolecular polypeptide solution to obtain antioxidation peptide powder. The preparation method has the beneficial effect of higher yield in preparation of the antioxidation peptide of the internal organs of the sea cucumbers.

Description

technical field [0001] The invention relates to the field of polypeptides, in particular to a preparation method for step-by-step and efficient preparation of sea cucumber visceral antioxidant peptides. Background technique [0002] Sea cucumbers are marine echinoderms belonging to the class Holothuroidea, and are widely distributed in oceans all over the world. Sea cucumber is as famous as ginseng, bird's nest and shark's fin. It is one of the eight treasures in the world. It can not only be used as food but also as a valuable medicinal material. According to the records in "Compendium of Materia Medica": Sea cucumbers are sweet and salty, tonify the kidneys, benefit the essence, take urine, aphrodisiac and treat impotence. Sea cucumber has the effects of improving memory, delaying gonad aging, preventing arteriosclerosis and anti-tumor. [0003] In terms of sea cucumber consumption, people usually feed on the body wall of sea cucumbers, while the internal organs of sea c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06
Inventor 曾红亮胡正红王锦锋张怡郑宝东
Owner SHENGTIAN FUQING FOOD