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Method for extracting and detecting rice blast genes of rice

A technology of rice blast and rice blast bacteria, which is applied in the field of rice blast, can solve the problems of not being able to accurately grasp rice blast gene data and affect the results of rice blast gene detection data, and achieve the goal of avoiding adverse effects, convenient comparison, and accurate results Effect

Inactive Publication Date: 2019-03-12
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting and detecting the rice blast gene, so as to solve the above-mentioned background technology. Certain impurities will affect the results of the detection data of the rice blast gene, and it is still impossible to accurately grasp the problem of the genetic data of each period of the rice blast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment 1 provides a method for extracting and detecting rice blast gene, and the above method will be described in detail below.

[0030] Before extracting the rice blast gene, prepare the DNA extraction buffer in advance. The DNA extraction buffer is a mixture of Tris-HCl, EDTA and TritonX-100 plus ddH2O. On the Pyricularia oryzae mycelium, pick an appropriate amount of Pyricularia oryzae mycelium, put it into a sterile test tube, and then inject the prepared DNA extraction buffer quantitatively into the sterile test tube according to the amount of Pyricularia oryzae mycelium In the test tube of the mycelia, make it fully mixed and contacted, and then, the opening at the upper end of the test tube is sealed to ensure the stability of the environment inside the test tube, so as to ensure the normal and safe extraction process of the rice blast gene.

[0031] After the test tubes with blast fungus mycelia are prepared, put the test tubes into a humid heat steril...

Embodiment 2

[0036] This embodiment 2 provides a method for extracting and detecting rice blast gene, and the above method will be described in detail below.

[0037] Before extracting the rice blast gene, prepare the DNA extraction buffer in advance. The DNA extraction buffer is a mixture of Tris-HCl, EDTA, KCl and Tween20, and then in a sterile environment, separate the Cut off the lesion and put it into a sterile test tube. Then, according to the amount of the plant lesion, quantitatively inject the prepared DNA extraction buffer into the test tube with the plant lesion, make it fully mixed and contact, and then put the test tube The opening at the upper end is sealed to ensure a stable environment inside the test tube, thereby ensuring a normal and safe extraction process of the rice blast gene.

[0038]After the test tubes with plant lesions are prepared, put the test tubes into a humid heat sterilizer for water bath treatment, and the solution in the test tubes should be completely i...

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PUM

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Abstract

The invention discloses a method for extracting and detecting rice blast genes of rice. The method is characterized by comprising the following steps of: cutting off scabs individually from plants ofdiseased rice or picking out the hyphae of rice blast fungi, putting into a test tube quantitatively, then injecting a DNA (deoxyribonucleic acid) extraction buffer solution into the test tube, putting the test tube in a damp and hot sterilization pot to perform water bath treatment for 10-12 min, naturally cooling the test tube subjected to the water bath treatment, then introducing into a corresponding filter device to filtrate, enabling a filtered mixed solution to be quantitatively and uniformly injected into a PCR-96 pore plate so as to be arranged in an environment of minus 20 DEG C forrefrigeration, collecting the data of the mixed solution in the PCR-96 pore plate through the probe of a detecting device, and generating a DNA detection data table automatically through a computer. The method for extracting and detecting the rice blast genes of the rice improves the controllability of rice blast gene extraction work, reduces the occurrence of accidents in the gene extraction work, and guarantees that the rice blast gene extraction work is stable and efficient.

Description

technical field [0001] The invention relates to the technical field of rice blast, in particular to a method for extracting and detecting rice blast genes. Background technique [0002] Rice is a kind of herbaceous Oryza genus, which belongs to cereals. It is also the most important and longest food in Oryza genus. With the increasing global population, people's demand for rice is increasing. However, due to the occurrence of various diseases in rice planting, which seriously restricts the output of rice, people are paying more and more attention to rice diseases. high. [0003] Rice blast is one of the important diseases of rice, which can cause a large reduction in yield, in severe cases, 40% to 50% reduction in yield, or even no harvest. It occurs in all rice regions in the world. The disease occurs everywhere in rice plants, among which the leaves and nodes of rice plants are the most common. After the occurrence, it can cause different degrees of yield reduction, espe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/04C12Q1/686C12R1/645
CPCC12Q1/6806C12Q1/686C12Q2527/125
Inventor 黄永相李伟郭建夫李东祝敏丹
Owner GUANGDONG OCEAN UNIVERSITY