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Vaccine antigen composition and preparation method thereof

A vaccine antigen and composition technology, applied in the field of vaccine research, can solve the problems of limited improvement of antigen stability, limited improvement of antigen activity, and many factors affecting stability, so as to avoid the reduction of antigen activity, be easy to prepare, and improve The effect of stability

Active Publication Date: 2019-03-15
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method is often used to improve the stability of protein drugs, but for virus particle antigens, its structure is more complex than that of general protein drugs, and there are more factors affecting its stability. Although adding inorganic salts can improve the stability of certain antigens to a certain extent stability, but inorganic salts have a limited effect on improving the stability of this antigen, and high concentrations of inorganic salts have a greater impact on the activity of the antigen; while the addition of proteins and sugars usually has a limited effect on improving the activity of the antigen, and the cost Higher, adding unnecessary macromolecular impurities at the same time, affecting the safety of the vaccine, there are still great difficulties in practical application

Method used

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  • Vaccine antigen composition and preparation method thereof
  • Vaccine antigen composition and preparation method thereof
  • Vaccine antigen composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of FMDV solution, its preparation method is as follows:

[0050] 1) A certain amount of choline-type ionic liquid [cho][Cl] and [cho][SO 4 ] were respectively dissolved in 20mM, pH7.5 phosphate buffer, and adjusted to pH 7.5 with 4mol / L NaOH or 2mol / L HCl to prepare a choline-type ionic liquid with a concentration of 1mol / L;

[0051] 2) To 1 mL of 100 μg / mL FMDV solution (pH 7.5), add 0.5 mL of the above ionic liquids [cho][Cl] and [cho][SO 4 ], mixing, to obtain the FMDV solution added with 0.5mol / L complex choline-type ionic liquid.

Embodiment 2

[0053] Compared with Example 1, except that the cholinergic isolated liquid is [cho][Cl] and [cho][HPO 4 ] except that other conditions are with embodiment 1.

Embodiment 3

[0055] Compared with Example 1, except that the choline-type isolated liquid is [cho][SO 4 ] and [cho] [Br], other conditions are the same as in Example 1.

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Abstract

The invention provides a vaccine antigen composition. The vaccine antigen composition comprises a vaccine antigen and choline-type ionic liquid. According to the vaccine antigen composition, through addition of the choline-type ionic liquid with good biocompatibility as an additive, the heat stability, the repeated freeze-thaw stability, the accelerated stability and the long-term storage stability of the vaccine antigen are significantly improved, the stability of the antigen in a solution and in an adjuvant environment is significantly improved and the antigen activity is not affected.

Description

technical field [0001] The invention belongs to the field of vaccine research, and relates to a vaccine antigen composition and a preparation method thereof, in particular to a vaccine antigen composition containing choline-type ionic liquid and a preparation method thereof. Background technique [0002] Antigens are the most important components of vaccines, and virus particle-based antigens are currently the most widely used type of antigens in the field of human vaccines and veterinary vaccines. Such antigens mainly include virus-like vaccine antigens and recombinant virus-like particle vaccine antigens. Such antigens usually have the characteristics of complex composition and large particle size in structure. The structure of natural virus particles is relatively stable, but after the virus particles used as antigens are attenuated, inactivated or reassembled, their natural structure is destroyed, their stability decreases, and they are extremely prone to cleavage or ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K9/08A61K39/39A61K47/18A61P31/14A61P31/20
CPCA61K39/12A61K39/39A61K47/186A61P31/14A61P31/20A61K9/08A61K2039/5258Y02A50/30
Inventor 张松平苏志国林旋杨延丽
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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