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Molecular marker primer for controlling QTL locus of corn ear length and application

A technology of molecular markers and ears of corn, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of slow, less reported progress, rare reports of ear length sites, etc., and achieve good results Breeding value, effect of increasing ear length

Active Publication Date: 2019-03-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the gene positional cloning of this trait is a research hotspot, the related genes of map position cloning in maize are rarely reported or the progress is slow, and the ear length loci with breeding value are rarely reported, so the research on related traits needs to be accelerated and fully evaluated. Its utilization value in breeding

Method used

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  • Molecular marker primer for controlling QTL locus of corn ear length and application
  • Molecular marker primer for controlling QTL locus of corn ear length and application
  • Molecular marker primer for controlling QTL locus of corn ear length and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Development of Molecular Markers Tightly Linked to Major QTL for Ear Length in Maize:

[0044] 1 Materials and methods

[0045] 1.1 Experimental materials

[0046] Maize Inbred Line W138, ZmEL-1 Near Isogenic Line W138 TY6 and the progeny mapping population constructed by the near isogenic line.

[0047] 1.2 Test method

[0048] 1.2.1 Target segment molecular marker development

[0049] According to the B73 genome, download the ZmEL-1 interval sequence, use the SSRSCAN tool of the REDB website to search for the SSR sequence, and then design specific primers based on the SSR flanking sequence, and then perform amplification based on the parent material W138 and TY6, and perform capillary electrophoresis Perform polymorphism analysis, and select markers with polymorphism and clear bands for population genotype analysis.

[0050] 1.2.2 Field experiments and identification of phenotypes

[0051] For the heterozygous recombinant single plant used for the offspring test...

Embodiment 2

[0088] The application of molecular marker primers closely linked to the major QTL ZmEL-1 of maize ear length in maize breeding:

[0089] 1. Materials and methods

[0090] 1.1 Experimental materials

[0091] Maize inbred line W138 and its near isogenic line W138 TY6 , 9 excellent corn inbred lines: HL78F (the male inbred line of Hualiang 78 cultivated by Hualiang Seed Industry), 501, HZ4, SHENG62 (the male inbred line of Shengrui 999 cultivated for Shengrui Company) , CHANG7-2, YE478, ZHENG58, 4CV, 6WC.

[0092] 1.2 Test method

[0093] 1.2.1 ZmEL-1 near-isogenic line W138 and its near-isogenic line W138 were selected based on molecular markers TY6 Hybrid combination with excellent inbred lines

[0094] Nine elite maize inbred lines (HL78F, 501, HZ4, SHENG62, CHANG7-2, YE478, ZHENG58, 4CV, 6WC) derived from different heterosis groups of maize were selected and selfed with the superior ZmEL-1 genotypes respectively. Line W138 (using molecular markers 1.10-131, 1.10-91 for...

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Abstract

The invention belongs to the biotechnical field and discloses a molecular marker primer for controlling a QTL locus of corn ear length and an application. The applicant identifies a novel main effectQTL ZmEL-1 for controlling corn ear length. The main effect QTL ZmEL-1 is positioned on a long arm of a first chromosome and is located between markers 1.10-131 and 1.10-91. The physical section is chr1:2799925380-283401913. The main effect QTL of controlling the ear length is found in the section for the first time and relatively high phenotype rate of contribution and additive effect exist, so that the primer has a potential breeding value. The locus can enhance the capacity characters such as ear length, row grain quantity, ear weight and ear grain weight obviously so as to achieve the effect of increasing the output. The developed molecular marker interlocked tightly with the QTL ZmEL-1 can be used for marker-assisted selection breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new molecular marker and application of a QTL locus for controlling ear length of corn. Background technique [0002] Maize yield is composed of the yield components of grains per ear and grain weight. Among them, the number of grains per panicle can be analyzed into related traits such as the number of rows per panicle and the number of kernels per row. Generally speaking, the longer the panicle length and the more grains in a row, the greater the possibility space for grain development. Locating QTLs for traits related to yield components, cloning key genes, and dissecting their genetic regulatory pathways have long been a research hotspot for molecular breeders. With the in-depth study of the maize genome, the innovation of molecular marker technology, and the continuous optimization of mapping population statistics methods, the current research on the location of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邱法展潘振远张祖新刘敏
Owner HUAZHONG AGRI UNIV
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