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Application of sandworm enzymatic hydrolyzate in the preparation of medicines or health care products for preventing and treating osteoarthritis

An osteoarthritis and sandworm enzyme technology, applied in the field of natural product applications, can solve the problems of lack of sandworm protein polypeptide anti-osteoarthritis activity and insufficient clarification of the pathogenesis, and achieves the promotion of chondrocyte proliferation, good application potential, The effect of reducing joint damage

Active Publication Date: 2022-03-15
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on OA, its pathogenesis is still not fully elucidated
However, there is no relevant report that the sandworm protein polypeptide has anti-osteoarthritis activity.

Method used

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  • Application of sandworm enzymatic hydrolyzate in the preparation of medicines or health care products for preventing and treating osteoarthritis
  • Application of sandworm enzymatic hydrolyzate in the preparation of medicines or health care products for preventing and treating osteoarthritis
  • Application of sandworm enzymatic hydrolyzate in the preparation of medicines or health care products for preventing and treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The effect of sandworm enzymatic hydrolysate on the proliferation of chondrocytes cultured in vitro

[0022] 1. Preparation of Sandworm Enzyme Hydrolysate for Cell Experiments

[0023] Take 50 mg of the lyophilized product of the enzymatic hydrolysate of sandworm and dissolve it in 10 mL of PBS solution to prepare a solution of the enzymatic hydrolysate of sandworm with a concentration of 5 mg / mL. The 0.22 μm micromembrane (SLGPo33RB disposable filter, Millipore) is sterile filtered. . Dilute with high-glucose DMEM medium (Thermo) step by step to obtain sandworm enzymolysate solution with concentrations of 2, 1, 0.5, 0.25, 0.125 mg / mL, and store in a -20°C refrigerator for later use.

[0024] 2. Isolation and culture of rat articular chondrocytes

[0025] Take 200g female SD rats (purchased from Guangdong Provincial Medical Laboratory Animal Center), kill them by dislocation, take the knee articular cartilage under sterile conditions, rinse with PBS, and cut...

Embodiment 2

[0030] Example 2: Interventional effect of sandworm enzymolysate on rat osteoarthritis

[0031] 1. Prepare sandworm enzymolysate for animal experiments

[0032] Take an appropriate amount of the lyophilized product of the enzymatic hydrolysate of sandworm and dissolve it in distilled water for intragastric administration to rats. The principle of preparation of the solution of sandworm enzymolysate is that the amount of each rat should not be more than 2 mL. And it needs to be freshly prepared before daily gavage administration.

[0033] 2. Modeling

[0034] Rats were anesthetized by intraperitoneal injection of chloral hydrate (350 mg / kg) (Shanghai Aladdin Biochemical Technology Co., Ltd.), and the knee joint of the right hind limb was routinely disinfected, and then a 1 mL injection needle was used to inject sterile physiological into the knee joint cavity through the patellar ligament. The suspension of 40 mg / mL kaolin and 20 mg / mL λ-carrageenan (Sigma) prepared in saline w...

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Abstract

The invention discloses the application of sandworm enzymatic hydrolyzate in the preparation of medicines or health care products for preventing and treating osteoarthritis. Pharmacological experiments have proved that sandworm enzymatic hydrolyzate can significantly improve the proliferation activity of chondrocytes cultured in vitro, and can significantly reduce bone and joint damage in rats, indicating that sandworm enzymatic hydrolyzate has the potential to treat or prevent osteoarthritis. The new application of the sandworm enzymatic hydrolyzate disclosed by the invention expands the indication or application range of the sandworm enzymatic hydrolyzate, and is expected to be developed into a new drug or health care product for preventing and treating osteoarthritis.

Description

technical field [0001] The invention relates to the technical field of natural product application, in particular to the application of seafood enzymolysis products in the preparation of medicines or health products. Background technique [0002] Osteoarthritis (OA) is a chronic degenerative joint disease characterized by progressive damage to articular cartilage, which occurs in weight-bearing and frequent joints. According to statistics, the incidence of OA in people over 60 years old is as high as 50%, and that in people over 75 years old is as high as 80%, and its disability rate is as high as 53%, which seriously threatens the health of residents. With the aging trend of my country's population, it is estimated that by 2050, my country's elderly over the age of 60 will reach 480 million, accounting for 34.1% of the total population, and will become the most aging country in the world. OA surgical treatment has problems such as high cost, pain, and prosthesis loosening ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/62A61P19/02A61P19/08A61P29/00A23L33/175A23L33/18
CPCA61P19/02A61P19/08A61P29/00A23L33/175A23L33/18A61K35/62A23V2002/00A23V2200/306A23V2200/316A23V2250/55A23V2250/06
Inventor 李立刘义吕应年吴科锋
Owner GUANGDONG MEDICAL UNIV
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