Unlock instant, AI-driven research and patent intelligence for your innovation.

Mesenchymal stem cell preservation solution for clinical local injection and method for preserving mesenchymal stem cells

A technology of local injection of stem cells, applied in the field of stem cell preservation solution, can solve the problems of low safety and short time for maintaining cell viability, and achieve the effect of a good living environment

Active Publication Date: 2021-11-16
CHENGDU QINGKE BIOTECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a clinical mesenchymal stem cell preservation solution and a method for preserving mesenchymal stem cells, which can effectively solve the problem of the low safety of the existing stem cell preservation solution and the time required to maintain cell viability. short question

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesenchymal stem cell preservation solution for clinical local injection and method for preserving mesenchymal stem cells
  • Mesenchymal stem cell preservation solution for clinical local injection and method for preserving mesenchymal stem cells
  • Mesenchymal stem cell preservation solution for clinical local injection and method for preserving mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A mesenchymal stem cell preservation solution for clinical local injection, comprising the following components in volume percentage:

[0034] 3% autologous plasma lysate, 3% autologous stem cell conditioned medium extract, 1% antioxidant vitamin C injection, 0.4% hyaluronic acid, and the rest is physiological saline injection, all of which are clinical injection grade reagents.

[0035] Wherein, the autologous plasma lysate is prepared by the following method:

[0036] (1) Use 8 mL sodium citrate anticoagulant tubes to collect 16 mL of peripheral blood from the patient in 2 tubes;

[0037] (2) Put the collected peripheral blood into a centrifuge and centrifuge at a speed of 1500r / min for 10 minutes. After centrifugation, the peripheral blood is divided into four layers, which are plasma layer, buffy coat layer, and gel layer from bottom to top. Buffy coat layer and blood cell layer, absorb the plasma layer and gently blow the buffy coat layer, then mix the plasma and ...

Embodiment 2

[0044] A mesenchymal stem cell preservation solution for clinical local injection, comprising the following components in volume percentage:

[0045] 1% autologous plasma lysate, 1% autologous stem cell conditioned medium extract, 0.1% antioxidant vitamin C injection, 0.1% hyaluronic acid, and the balance is physiological saline injection.

[0046] The preparation method of autologous plasma lysate and autologous stem cell conditioned medium extract is the same as that in Example 1.

Embodiment 3

[0048] A mesenchymal stem cell preservation solution for clinical local injection, comprising the following components in volume percentage:

[0049] Autologous plasma lysate 5%, autologous stem cell conditioned medium extract 5%, antioxidant vitamin C injection 1%, hyaluronic acid 0.4%, and the balance is physiological saline injection.

[0050] The preparation method of autologous plasma lysate and autologous stem cell conditioned medium extract is the same as that in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a mesenchymal stem cell preservation solution for clinical local injection and a method for preserving mesenchymal stem cells. The preservation solution comprises the following components in volume percentage: autologous plasma lysate 1-5%, autologous stem cell conditioned medium extraction 1-5% of anti-oxidant, 0.1-1% of antioxidant, 0.1-0.4% of hyaluronic acid, and the rest is normal saline injection. After the mesenchymal stem cells are preserved by using the preservation solution, the cell viability rate is high, the cell viability is maintained for a long time, and there is no exogenous component in the preservation solution, so the safety is high.

Description

technical field [0001] The invention belongs to the technical field of stem cell preservation solution, and in particular relates to a mesenchymal stem cell preservation solution for clinical local injection and a method for preserving mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are derived from the mesoderm in the early stages of development. MSCs were discovered in the bone marrow in 1968, and they were found to have the characteristics of multidirectional differentiation potential / hematopoietic support and promotion of stem cell implantation / immune regulation and self-replication And increasingly people's attention. [0003] Cell therapy can repair damaged cells, replace necrotic cells, activate dormant cells, and regenerate fresh cells. After stem cells are injected into the human body, they will secrete cytokines, which can promote cell growth. The secreted growth factors bind to aging cell receptors, enhance cell activity, and d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 高雪华海泉陈静娴赵峻
Owner CHENGDU QINGKE BIOTECH