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Method for processing blood sample for HPV genotype nucleic acid detection

A specimen processing and sample technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as experimental failure, waste of personnel and reagent costs, increase the pain of patient sampling, etc., to avoid false negative results, Improve the accuracy and ensure the effect of the success rate of the experiment

Inactive Publication Date: 2019-03-26
JILIN JINYU MEDICAL SCI INSPECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the cervical exfoliated cell sample contains blood, the blood sample will be affected by blood cells during the test, which will inhibit the extraction of HPV DNA, resulting in false negative results, or at least the experiment fails, and the patient needs to be re-sampled, causing personnel and The waste of reagent costs increases the pain of sampling for patients, and in serious cases causes misjudgment of results and delays the best time for patients to receive treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 16 cases of HPV samples distributed in four types of blood samples, and classify them according to the number of blood cells. After the samples are classified, add perspective cell preservation solution according to the number of red blood cells for pretreatment. Processing method: take 1000 μl of type A cervical exfoliated cells and add them to 1.5ml EP tube It is directly used in the experiment. Take 650 μl of the type B cervical exfoliated cell sample and add it to a 1.5ml EP tube, add 350 μl of the exfoliated cell preservation solution and mix well and use it for the experiment. Take 450 μl of the C type cervical exfoliated cell sample and add it to the 1.5ml EP tube and add 550 μl of the exfoliated cell preservation solution After mixing, it was used in the experiment. Take 250 μl of the type D cervical exfoliated cell sample and add it to a 1.5ml EP tube, add 750 μl of the exfoliated cell preservation solution, mix well and use it in the experiment.

[0032] T...

Embodiment 2

[0035] In addition, 16 cases of HPV samples distributed in four types of blood samples were selected and classified according to the number of blood cells contained. After the samples were classified, they were pretreated according to the number of red blood cells by adding perspective cell preservation solution. Processing method: take 1000 μl of type A cervical exfoliated cell samples and add 1.5ml EP The tube is used for experiments. Take 600 μl of the type B cervical exfoliated cell sample and add it to a 1.5ml EP tube, add 400 μl of the exfoliated cell preservation solution and mix well and use it for the experiment. Take 400 μl of the C type cervical exfoliated cell sample and add it to the 1.5ml EP tube and add 600 μl of the exfoliated cell preservation solution After mixing, it was used in the experiment. Take 200 μl of the type D cervical exfoliated cell sample and add it to a 1.5ml EP tube, add 800 μl of the exfoliated cell preservation solution, mix well and use it fo...

Embodiment 3

[0040] Another 16 cases of HPV samples distributed in four types of blood samples were selected and classified according to the number of blood cells contained. After the samples were classified, they were pretreated by adding perspective cell preservation solution according to the number of red blood cells. The mlEP tube is used for the experiment, 550 μl of the exfoliated cervical cell sample of type B is added to a 1.5ml EP tube, and 450 μl of the exfoliated cell preservation solution is added and mixed for use in the experiment. 550 μl was mixed and used for the experiment, and 150 μl of the type D cervical exfoliated cell sample was added to a 1.5ml EP tube, and 750 μl of the exfoliated cell preservation solution was added to be mixed and used for the experiment.

[0041] Take the EP tubes of the above four types of blood samples, centrifuge at 13200rpm for 10min at high speed, discard the supernatant, add 200μl exfoliated cell nucleic acid release agent, shake and mix wel...

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Abstract

The invention discloses a method for processing a blood sample for HPV genotype nucleic acid detection, belonging to the technical field of in-vitro detection. According to the method, the technical problems a blood-containing sample is influenced by blood cells in the detection process, the extraction of HPVDNA is restrained, and a false negative result occurs are solved. According to the method,cervical exfoliated cell samples are classified according to the number of red cells, exfoliated cell preserving fluid is added for preprocessing, the red cells are adequately suspended and dispersedin the premise that the forms and nucleic acids of the cervical exfoliated cells are complete, the red cells are scoured off, and the Globin signal value of the sample is more than 150, so that the experimental success rate is guaranteed, the false negative result is avoided, and the accuracy of a detection result is improved.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a blood sample processing method for HPV typing nucleic acid detection. Background technique [0002] Cervical cancer ranks second in the incidence and mortality of female malignant tumors in my country, with 465,000 new cancer patients each year. Studies have found that HPV can be detected in about 90% of cervical cancer patients. HPV is human papilloma virus (humanpapilloma virus), which is the pathogen that causes cervical cancer. HPV is divided into 27 subtypes (6, 11, 16, 18, 26 , 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 55, 56, 58, 59, 61, 66, 68, 81, 82, 83), among them, HPV- 16 and HPV-18 are high-risk types, which are prone to cause invasive tumors in the lower vagina. Therefore, HPV typing detection can prevent cervical cancer very well. [0003] In the prior art, flow cytometry fluorescent hybridization technology is commonly used to detect 27 HPV s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 朱爱丽周婷婷许友强李雨艳刘明珠张馨月
Owner JILIN JINYU MEDICAL SCI INSPECTION CO LTD
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