Application of miR-296 and simulant thereof in promoting osteogenic differentiation of bone marrow mesenchymal stem cells and bone formation

A technology for bone marrow mesenchymal and osteogenic differentiation, applied in the field of medicine, can solve problems such as many complications, and achieve the effect of increasing bone formation and promoting the osteogenic differentiation of bone marrow mesenchymal stem cells
CN109528751AActive Publication Date: 2019-03-29HARBIN MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN MEDICAL UNIVERSITY
Publication Date
2019-03-29

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Abstract

The invention discloses an application of miR-296 and a simulant thereof in promoting osteogenic differentiation of bone marrow mesenchymal stem cells and bone formation. The regulation effect of themiR-296 on osteogenic differentiation of the bone marrow mesenchymal stem cells and bone formation is explored through in-vitro experiments. In-vivo experiments prove that the formation of new bones can be promoted through the transfection of the miR-296, and the in-vitro experiments prove that the miR-296 can improve osteogenic differentiation ability of the bone marrow mesenchymal stem cells andpromote in-vivo bone formation by promoting the formation of osteoblast calcium nodules and the activity of a marker enzyme ALP, as well as promoting the expression of Osterix and Runx2 which are important regulatory transcription factors in the process of osteogenic differentiation. The invention provides a new technical means for the treatment of osteoporosis.
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Description

technical field

[0001] The present invention relates to a new therapeutic application of miR-296, in particular to the application of miR-296 and its mimics in promoting osteogenic differentiation and bone formation of bone marrow mesenchymal stem cells. The invention belongs to the technical field of medicine. Background technique

[0002] Osteoporosis, or osteoporosis, is a metabolic bone lesion characterized by loss of bone mass per unit volume caused by various reasons. This disease is seen in postmenopausal women and the elderly. It often manifests as bone pain, increased bone fragility, and prone to fractures, causing serious damage to the human body. At present, mild symptoms can be improved through diet control, exercise, and appropriate supplementation of calcium and vitamin D. For those with signs of osteoporosis or fractures, drugs that resist bone resorption or promote bone formation are often used to improve symptoms , Severe cases need to be supplemented by s...

Claims

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