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Interference fragment and application thereof

A fragment and gene technology, applied in the field of interference fragment and its application, can solve the problem that there is no cure for the root cause, achieve important market value and significance, and alleviate the effect of corresponding symptoms

Active Publication Date: 2019-03-29
广西壮族自治区生殖医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed clinically that the occurrence of OHSS is related to the stimulation of too many follicles and the application of human chorionic gonadotropin (HCG) to induce ovulation. Many factors are involved in the occurrence process. At present, there is still no one theory that can explain all the symptoms of OHSS. problem, and there is no thorough prevention and effective cure

Method used

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  • Interference fragment and application thereof
  • Interference fragment and application thereof
  • Interference fragment and application thereof

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Experimental program
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Effect test

Embodiment

[0047] 1. Design the AQP1 siRNA sequence, and design the corresponding negative control siRNA, and then submit it to the gene company for synthesis; among them,

[0048] AQP1 RNAi sequence:

[0049] Positive sequence: 5'-GCUGUACUCAUCUACGACUTT-3';

[0050] Reverse sequence: 5'-AGUCGUAGAUGAGUACAGCTT-3'.

[0051] 2. Aliquot all AQP1 siRNA sequences and negative control interference sequences into 1OD / tube, centrifuge at 3000-4000rpm for 3min before use; then add 125μL DEPC H 2 O resuspended to prepare a stock solution with a final concentration of 20 μM.

[0052] 3. Human umbilical vein endothelial cells (HUVEC cells) use improved complete medium (MCDB131 medium + 20% fetal bovine serum + 50mg / mL endothelial growth factor ECGS + 100U / mL penicillin + 50μg / mL heparin + 2mmol glutamine +100U / mL Streptomycin) after recovery, trypsinized and replanted in 6-cell culture plate; 37°C, 5% CO 2 After culturing overnight, start the cell transfection experiment when the coverage rate of th...

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Abstract

The present invention belongs to the technical field of bioengineering and particularly relates to an interference fragment used for interfering with a transcription level of AQP1 gene and an application thereof. A sequence of the interference fragment is as follows: AQP1 RNAi-FP: 5'-GCUGUACUCAUCUACGACUTT-3'; and AQP1 RNAi-RP: 5'-AGUCGUAGAUGAGUACAGCTT-3'. The interference fragment is used to interfere with the transcription level of the AQP1 gene, and can be used to prepare medicines and kits for alleviating corresponding symptoms of ovarian hyperstimulation syndrome. An applicant can significantly inhibit the transcription level of the AQP1 gene by designing the interference fragment, so that the expression of the AQP1 gene is significantly decreased, thereby achieving a purpose of alleviating the corresponding symptoms of the ovarian hyperstimulation syndrome (OHSS), and the interference fragment has important market value and significance.

Description

【Technical field】 [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an interference fragment and its application. 【Background technique】 [0002] Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic self-limiting disease caused by ovulation induction with exogenous gonadotropins. In patients receiving controlled ovulation, the overall incidence of OHSS It is within 14%, and the severity is about 0.5-2%. With the widespread development of assisted reproductive technology and the increase in the use of ovulation-stimulating drugs, the incidence of OHSS tends to increase. OHSS can cause acute extravasation of a large amount of protein-rich plasma in the body, causing ascites, pleural effusion, and even diffuse edema. Hemoconcentration, hypovolemia, secondary renal failure, water and electrolyte disturbance, azotemia, and hypovolemic shock may occur , and even thrombosis. The clinical manifestations are gastrointestinal d...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/87C12N5/10
CPCC12N5/069C12N15/113C12N15/87C12N2510/00C12N2310/14
Inventor 莫毅梁方方李云娟吕福通牛向丽徐亦辰王娟谢丹尼叶健莫云
Owner 广西壮族自治区生殖医院