Method for extracting kaempferol glucoside compounds from Nanshan tea

A technology for glucoside and compound, applied in the field of compound extraction, can solve problems such as insufficient research, and achieve the effect of simple extraction method and high extraction purity

Active Publication Date: 2021-07-13
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, not enough research has been done on the specific components in the extract

Method used

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  • Method for extracting kaempferol glucoside compounds from Nanshan tea
  • Method for extracting kaempferol glucoside compounds from Nanshan tea
  • Method for extracting kaempferol glucoside compounds from Nanshan tea

Examples

Experimental program
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Effect test

Embodiment 1

[0052] The tea fruit of Nanshan tea is dried in the air, shelled, and the cake produced after removing oil by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea cake. Ultrasonic extraction with 50% ethanol aqueous solution at 60°C for 3 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), and then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 20 times with water to obtain a sample liquid.

[0053] Shelling and degreasing are to maximize the extraction yield and reduce interference during the separation process. Appropriate raw material handling methods are also beneficial to avoid the loss of target compounds.

[0054] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no et...

Embodiment 2

[0059] The tea fruit of Nanshan tea is dried in the air, shelled, and the cake produced after removing oil by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching with 60% ethanol aqueous solution at 70°C twice, each time for 2 hours, with a solid-liquid ratio of 1:2 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 22 times with water to obtain a sample liquid.

[0060] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of BV 1 The volume fractio...

Embodiment 3

[0064] The tea fruit of Nanshan tea is dried in the air, shelled, and the cake produced after removing oil by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching with 55% ethanol aqueous solution at 65°C for 4 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 18 times with water to obtain a sample liquid.

[0065] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of BV 1 The volume fract...

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Abstract

The invention provides a method for extracting kaempferol glucoside compounds from Nanshan tea. The method is as follows: the Nanshan tea cake is extracted to obtain a sample liquid; the sample liquid is wet-loaded to a macroporous resin chromatography column, and the ethanol aqueous solution system is used for gradient elution, and the eluent containing the target compound is collected. The crude component liquid is obtained after post-processing; the crude component liquid is wet loaded to the polyamide resin chromatography column, and gradient elution is performed, and the eluate containing the target compound is collected through chromatographic detection, concentrated and freeze-dried to obtain the crude extract The crude extract was dissolved in a solvent and injected, and the first C18ME chromatographic column was used for HPLC semi-preparation, and acetonitrile-formic acid aqueous solution was used as the mobile phase for isocratic elution, and the fractions containing the target compound were collected respectively, concentrated and frozen. Dry to obtain the first and second target compounds. The method provided in the application effectively separates the target compound from the Camellia plant, and the extraction purity is high.

Description

technical field [0001] The invention relates to the field of compound extraction, in particular to a method for extracting kaempferol glucoside compounds from Nanshan tea. Background technique [0002] Nanshan tea, also known as Guangning safflower oil tea (Camellia semiserrata Chi.), belongs to the plant of the genus Camellia (Camellia L.) of the family Theaceae (Theaceae), and is a perennial tree or shrub. Nanshan tea is mainly distributed in the southeast of Guangxi and the west of Guangdong in China. It is an important cultivated species of Camellia oleifera. After research and comparison, it is found that among all kinds of Camellia oleifera, the chemical components in Guangning safflower Camellia oleifera are relatively rich. [0003] Nanshan tea and its cakes are especially rich in compounds such as flavonoids and saponins with various biological activities. At present, the research on Camellia oleifera cake mainly focuses on the optimization of various extraction ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 曹清明胡丹丹师明月欧青媛周文化
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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