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A kind of imperfect amphiphilic peptide w4 and its preparation method and application

An amphiphilic peptide, perfect technology, applied in the field of non-perfect amphiphilic peptide W4 and its preparation, can solve the problems of weak antibacterial activity, high synthesis cost, protease sensitivity, etc., to improve development potential, increase selectivity, Effect of low hemolytic activity

Active Publication Date: 2021-10-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural cationic antimicrobial peptides are not perfect. Most natural antimicrobial peptides have weak antibacterial activity, relatively narrow antibacterial spectrum, high synthesis cost, some antimicrobial peptides have certain toxicity to eukaryotes, and are highly effective against pathogenic microorganisms. Killing activity is often accompanied by hemolysis to eukaryotes and sensitivity to proteases

Method used

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  • A kind of imperfect amphiphilic peptide w4 and its preparation method and application
  • A kind of imperfect amphiphilic peptide w4 and its preparation method and application
  • A kind of imperfect amphiphilic peptide w4 and its preparation method and application

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Effect test

Embodiment 1

[0015] Design of Antimicrobial Peptides

[0016] Select 4 arginines as positively charged amino acids and 5 tryptophans as hydrophobic amino acids, two arginines are inserted into tryptophan as a unit, and the projection of the helical wheel forms a "four-sided" conformation, that is, two hydrophobic surfaces and two A hydrophilic surface, and four arginines form two pairs of intramolecular hydrogen bonds. The derived peptide is named W4, and its sequence is shown in the table below.

[0017] Table 1 Amino Acid Sequence of Derived Peptides

[0018]

[0019] The charges of W4 are +4 respectively, and the hydrophobic value is 0.801. The carboxyl terminus of W4 was amidated to add a positive charge and increase the stability of the peptide. In the case of improving the bactericidal activity of the antimicrobial peptide, the hemolytic activity of the antimicrobial peptide is reduced, the selectivity of the antimicrobial peptide between bacterial cells and mammalian cells is i...

Embodiment 2

[0021] Synthesis of W4 Antimicrobial Peptide by Solid Phase Chemical Synthesis

[0022] 1. The preparation of antimicrobial peptides is carried out one by one from the C-terminal to the N-terminal, and is completed by a peptide synthesizer. First, Fmoc-X (X is the first amino acid at the C-terminal of each antimicrobial peptide) is inserted into Wang resin, and then the Fmoc group is removed to obtain X-Wang resin; then Fmoc-Y-Trt-OH (9 -Fmoxy-trimethyl-Y, Y is the second amino acid at the C-terminus of each antimicrobial peptide); according to this procedure, it is synthesized from the C-terminus to the N-terminus until the synthesis is completed, and the side of the Fmoc group is removed chain protection resin;

[0023] 2. Add a cleavage reagent to the peptide resin obtained above, react for 2 hours at 20°C in the dark, filter; wash the precipitate with TFA (trifluoroacetic acid), mix the washing liquid with the above filtrate, concentrate with a rotary evaporator, and then...

Embodiment 3

[0037] Embodiment 3: the mensuration of antimicrobial peptide antibacterial activity

[0038] 1. Determination of antibacterial activity: Prepare the peptide as a storage solution for use. The minimum inhibitory concentrations of several antimicrobial peptides were determined by the broth microdilution method. Using 0.01% acetic acid (containing 0.2% BSA) as the diluent, a series of gradient antimicrobial peptide solutions were sequentially prepared using the double dilution method. Take 100 μL of the above solution and place it in a 96-well cell culture plate, then add an equal volume of the bacteria solution to be tested (~10 5 individual / mL) in each well. Positive controls (containing bacterial fluid but not antimicrobial peptides) and negative controls (neither bacterial fluid nor peptides) were set up. Incubate at a constant temperature of 37°C for 20 hours, and the minimum inhibitory concentration is the one where no turbidity is seen at the bottom of the well with th...

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Abstract

The invention provides an imperfect amphiphilic peptide W4 and its preparation method and application. The sequence of the imperfect amphiphilic peptide W4 is shown in SEQ ID No.1. Preparation method: select 4 arginines as positively charged amino acids and 5 tryptophans as hydrophobic amino acids, two arginines are inserted into tryptophan as a unit, and the spiral wheel projection forms a "four-sided" conformation, that is, two hydrophobic Surface and two hydrophilic surfaces, and four arginines form two pairs of intramolecular hydrogen bonds. The derived peptide is named W4. The present invention greatly reduces the hemolytic activity of the antimicrobial peptide while improving the bactericidal activity of the antimicrobial peptide. Improved selectivity of antimicrobial peptides between bacterial cells and mammalian cells, increasing their potential for development as an alternative to antibiotics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an imperfect amphiphilic peptide W4 and its preparation method and application. Background technique [0002] The application of antibiotics as feed additives can be traced back to 1943. Americans first discovered that some antibiotic fermentation residues can promote the growth of pigs. In 1949, Americans Cunha and Stokstad first applied penicillin to conduct systematic comparative experiments in animals. [0003] The results confirmed that the application of antibiotics has good disease prevention and growth promotion effects on animals. In 1950, the U.S. Food and Drug Administration officially approved the application of penicillin and other antibiotics in feed. Because it can promote the growth of animals, increase the rate of weight gain, improve the reproductive performance of animals, increase the survival rate, reduce the incidence of disease, improve the utiliz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/04C07K1/06A61K38/08A61P31/04
CPCA61K38/00A61P31/04C07K7/06
Inventor 单安山何诗琪杨占一王家俊
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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