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Oyster polysaccharide and extraction method and application thereof

An extraction method and technology of oyster polysaccharides, applied in the field of oyster polysaccharides and its extraction, can solve the problems of high purity, low extraction rate, and high difficulty, and achieve the effects of high purity, easy availability of raw materials, and resource saving

Active Publication Date: 2019-04-02
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of oyster polysaccharides has low extraction rate and high purity, especially the difficulty of obtaining oyster polysaccharides with anti-tumor effect.

Method used

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  • Oyster polysaccharide and extraction method and application thereof
  • Oyster polysaccharide and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This example provides an oyster polysaccharide and its extraction method. Specific steps are as follows:

[0033] Weigh 4Kg of oyster meat (containing 154.9g of glycogen) and wash it, add 12Kg of water, and homogenate; adjust the pH value of the homogenate to 7.0, and the temperature is 37°C, add 80g of subtilisin and 80g of Aspergillus oryzae protease to hydrolyze 8 After 1 hour, the temperature was raised to 80°C, and after keeping for 5-10 minutes, the temperature was lowered to 25°C.

[0034] Add 40g of isoamylase to the clear liquid, adjust the pH value of the clear liquid to 6.5, enzymolysis temperature is 45°C, boil for 10 minutes after the enzymatic hydrolysis reaction for 3 hours, cool to room temperature, centrifuge, and take the clear liquid.

[0035] With a molecular weight cut-off of 1×10 4 Da-6×10 4 The Da membrane separates the supernatant to obtain a retentate.

[0036] Chloroform and n-butanol are mixed at a volume of 4:1, and stored in a brown bott...

Embodiment 2

[0039] This example provides an oyster polysaccharide and its extraction method. Specific steps are as follows:

[0040] Weigh 4Kg of oyster meat (containing 154.9g of glycogen) and wash it, add 12Kg of water, and homogenate; adjust the pH value of the homogenate to 6.5, and the temperature is 37°C, add 60g of subtilisin and 100g of Aspergillus oryzae protease to hydrolyze 8 After 1 hour, the temperature was raised to 80°C, and after keeping for 5-10 minutes, the temperature was lowered to 25°C.

[0041]Add 30 g of isoamylase to the clear liquid, adjust the pH value of the clear liquid to 7.0, enzymolysis temperature is 45°C, boil for 10 minutes after the enzymatic hydrolysis reaction for 3 hours, cool to room temperature, centrifuge, and take the clear liquid.

[0042] With a molecular weight cut-off of 1×10 4 Da-6×10 4 The Da membrane separates the supernatant to obtain a retentate.

[0043] Chloroform and n-butanol are mixed at a volume of 4:1, and stored in a brown bot...

Embodiment 3

[0046] This example provides an oyster polysaccharide and its extraction method. Specific steps are as follows:

[0047] Weigh 4Kg of oyster meat (containing 154.9g of glycogen) and wash it, add 12Kg of water, and homogenate; adjust the pH value of the homogenate to 7.0, and the temperature is 37°C, add 100g of subtilisin and 60g of Aspergillus oryzae protease to hydrolyze 8 After 1 hour, the temperature was raised to 80°C, and after keeping for 5-10 minutes, the temperature was lowered to 20°C.

[0048] Add 50 g of isoamylase to the clear liquid, adjust the pH value of the clear liquid to 6.5, enzymolysis temperature is 50°C, boil for 10 minutes after the enzymatic hydrolysis reaction for 3 hours, cool to room temperature, centrifuge, and take the clear liquid.

[0049] With a molecular weight cut-off of 1×10 4 Da-6×10 4 The Da membrane separates the supernatant to obtain a retentate.

[0050] Chloroform and n-butanol are mixed at a volume of 4:1, and stored in a brown bo...

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Abstract

The invention relates to oyster polysaccharide and an extraction method and application thereof. The extraction method comprises the steps that when oyster meat is subjected to an enzymolysis reaction, used enzymes comprise subtilisin and aspergillus oryzae proteases, wherein the consumption ratio of the subtilisin to the aspergillus oryzae proteases is (1-5) to (5-1); and the used enzymes furthercomprise isoamylase, wherein the consumption ratio of the isoamylase to the total amount of the subtilisin and the aspergillus oryzae proteases is (0.5-2) to (2-10). The oyster polysaccharide disclosed by the invention has the beneficial effects that the oyster polysaccharide obtained by the method is high in yield and high in purity. The oyster polysaccharide has highly significant restraining effect on tumor cells. The provided method is simple and easy to operate, resources are saved, and the raw materials are easy to obtain.

Description

technical field [0001] The invention relates to an oyster polysaccharide and its extraction method and application, belonging to the field of traditional Chinese medicine extraction. Background technique [0002] Oysters, belonging to Oysteridae or Slataceae, bivalve molluscs, are distributed in coastal waters of temperate and tropical oceans. Oysters are widely distributed in my country's coastal areas, and are produced in the Bohai Sea, the Yellow Sea to the Nansha Islands, and there are about 20 species. Oyster meat is fat and smooth, delicious and nutritious, known as "undersea milk". At present, in addition to using oysters as not yet directly edible, a large number of products such as health food, food, and seasonings with oysters as main components have also been developed in a large number. With the confirmation of the effectiveness of oyster extract and oyster functional food and health food, its application scope will continue to expand. The prospect of researchi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/16C12P19/04C08B37/00A61P35/00
CPCA61P35/00C08B37/0003C12P19/04C12P19/16
Inventor 石清东王姣王正元
Owner 北京颐方生物科技有限公司