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Method for intensifying fermentation process of tobacco leaves

A fermentation process and tobacco leaf technology, applied in tobacco, tobacco processing, application, etc., can solve problems affecting the safety of snus consumption, and achieve the effects of improving practical safety, increasing fineness, and improving smoke quality

Inactive Publication Date: 2019-04-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, snus is a smokeless tobacco product made from tobacco, which contains all the chemical components of tobacco, so some inherent harmful components in tobacco will seriously affect the safety of snus consumption

Method used

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  • Method for intensifying fermentation process of tobacco leaves
  • Method for intensifying fermentation process of tobacco leaves
  • Method for intensifying fermentation process of tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This embodiment is the preparation of Bacillus amyloliquefaciens GUHP-86 preparation, which specifically includes the following steps:

[0024] (1) Weigh 1.25g of tryptone, 0.625g of yeast extract, 6.25g of NaCl, and 0.625g of glucose, add distilled water to 1000mL, and sterilize by high-pressure steam at 121°C for 20min, and insert the Bacillus amyloliquefaciens after cooling GUHP-86 was cultured at 37°C and 180r / min for 18h±2h to obtain seed liquid;

[0025] (2) centrifuge 1000mL of the seed liquid at 4°C and 8000r for 10min, collect the precipitate, and wash the precipitate with 8.5g / L sterilized physiological saline, then add 1000mL of sterilized physiological saline to the precipitate, Shake well to obtain the Bacillus amyloliquefaciens GUHP-86 preparation.

[0026] The Bacillus amyloliquefaciens GUHP-86 was preserved in China Center for Type Culture Collection on January 4, 2016, with the preservation number CCTCC M 2016003.

[0027] The sterilized physiological...

Embodiment 2

[0029] In the present embodiment, take 20g tobacco leaf in the sealed bag of 14cm * 20cm specification, draw 2mL described Bacillus amyloliquefaciens GUHP-86 preparation (being 10% inoculum size) in tobacco leaf, 4mL has sugar-salt solution in tobacco leaf, fully Mix well to obtain sample 1.

[0030] The preparation steps of the sugar-salt solution are as follows: Weigh the MgCl 2 5g, KCl 0.5g, CaCl 2 0.5g, 0.5g NaCl, 2g glucose, add water to 1000mL, dissolve to obtain a sugar-salt solution.

[0031] Weigh 20g of tobacco leaves into a sealed bag with a size of 14cm×20cm, draw 2mL of sterile physiological saline and 4mL of sugar-salt solution into the tobacco leaves, and mix well to obtain Comparative Sample 1.

[0032] The sample 1 and the comparison sample 1 were cultured in the 37°C incubator at the same time, and the ventilation was carried out regularly every day, and when the humidity decreased, there must be added sugar and salt solution.

[0033] The sensory evalua...

Embodiment 3

[0036]In the present embodiment, take 20g tobacco leaf in the sealed bag of 14cm * 20cm specification, draw 4mL described Bacillus amyloliquefaciens GUHP-86 preparation (being 20% ​​inoculum size) in tobacco leaf, 4mL has sugar-salt solution in tobacco leaf, fully Mix well to obtain sample 2.

[0037] The preparation steps of the sugar-salt solution are as follows: Weigh the MgCl 2 5g, KCl 0.5g, CaCl 2 0.5g, 0.5g NaCl, 2g glucose, add water to 1000mL, dissolve to obtain a sugar-salt solution.

[0038] Weigh 20g of tobacco leaves into a sealed bag with a size of 14cm×20cm, draw 4mL of sterile physiological saline and 4mL of sugar-salt solution into the tobacco leaves, and mix well to obtain Comparative Sample 2.

[0039] The sample 2 and the comparison sample 2 were cultivated in the incubator at 37°C at the same time, and the ventilation was carried out regularly every day, and when the humidity decreased, a sugar-salt solution must be added.

[0040] More than 10 sensory...

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Abstract

The invention discloses a method for intensifying fermentation process of tobacco leaves. The tobacco leaves used in the fermentation process are treated by using a bacillus amyloliquefaciens GUHP-86preparation, wherein the bacillus amyloliquefaciens GUHP-86 preparation comprises bacillus amyloliquefaciens GUHP-86, the bacillus amyloliquefaciens GUHP-86 is preserved in China Center for Type Culture Collection on Jan. 4th, 2016, and the preservation number is CCTCC M 2016003. The fermentation process of tobacco cut stems can be intensified by using the bacillus amyloliquefaciens GUHP-86 preparation, the tobacco fragrance is increased, the tobacco gas is soft, the fine and smooth characteristics are increased, the irritation is reduced, the smoke quality is improved obviously, and the practicability and safety of mouth tobaccos are improved. Moreover, the invention also discloses a method for improving the activity of protease and amylase in the fermentation process of the tobacco leaves.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and tobacco processing, and in particular relates to a method for strengthening the fermentation process of tobacco leaves. Background technique [0002] Smokeless tobacco products (STPs) do not undergo tobacco combustion process, do not produce harmful pyrolysis products, produce no smoke, and can be used in public places. They are an important form of low-harm and environmentally friendly new tobacco products. [0003] Snus is an important component of STPs, and is currently the product category with the fastest growing market share. It has attracted the attention of major tobacco companies in the world, and has invested a lot of money and energy in related research and development in this field. [0004] Snus products are generally used in the oral cavity in the form of tobacco powder, and the components in the tobacco are dissolved and absorbed through the oral mucosa. Therefore, tobac...

Claims

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Application Information

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IPC IPC(8): A24B15/20A24B13/00
CPCA24B15/20A24B13/00
Inventor 田永峰杨柳王昆淼朱瑞芝段沅杏赵伟赵杨韩熠张霞刘志华陈永宽缪明明
Owner CHINA TOBACCO YUNNAN IND
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