The invention relates to a black chokeberry fruit wine which is prepared from the following raw materials in parts by weight: 105-130 parts of apple, 135-165 parts of black chokeberry, 135-165 parts of white granulated sugar, 0.09-0.11 part of food-grade sodium metabisulfite, 0.27-0.33 part of pectinase, 0.025-0.035 part of yeast powder, 0.45-0.55 part of yeast nutrient material and 0.09-0.11 part of amylase. The preparation method comprises the following steps: taking the apples, and pressing to obtain crude apple juice; carrying out enzyme treatment on the crude apple juice, filtering, and sterilizing to obtain sterilized apple juice; and taking the black chokeberry, crushing, adding the white granulated sugar, water, the rest pectinase, yeast powder, yeast nutrient material and food-grade sodium metabisulfite, uniformly mixing, fermenting, filtering, adding the sterilized apple juice into the obtained liquid, fermenting, filtering, taking the clear solution, and carrying out high static pressure treatment, thereby obtaining the black chokeberry fruit wine. The black chokeberry fruit wine has pure vinous flavor and natural aroma of the raw material fruits, and has the health care functions of resisting oxidation, lowering the blood sugar, softening the blood vessels and the like.