Breeding process of enoki mushrooms liquid strain

A technology of liquid strains and Flammulina velutipes, applied to fungi, using electricity/wave energy to treat microorganisms, electricity/wave energy to treat enzymes, etc., can solve problems such as mutual adhesion and long breeding cycle, and achieve increased vibration frequency and increased adsorption Ability to improve taste

Inactive Publication Date: 2019-04-12
SHANDONG YOUHE BACTERIA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding methods of Flammulina velutipes mainly include hybrid breeding, mutation breeding and genetic engineering breeding, etc. However, the disadvantages of these methods are: the breeding cycle is long, and it takes 3-5 years to breed a new variety, requiring a large amount of raw materials and facilities and equipment , it is also easier to cause mutual attachment between spores of different strains

Method used

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  • Breeding process of enoki mushrooms liquid strain
  • Breeding process of enoki mushrooms liquid strain
  • Breeding process of enoki mushrooms liquid strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Step a, in a sterile environment, take out the germs, inoculate them in mycelium medium, and culture them statically at 20°C for 8 days; the mycelium medium contains the following weight of raw materials per 1000ml: 30g of maltose, Peptone 10g, add water to make up to 1000ml. The preparation method is: put 1000ml of water in the pot, add peptone and maltose, then add water to make up to 1000ml, put it in a sealed bottle, and sterilize it in an autoclave at 110°C for 25 minutes;

[0062] Step b, take 5 g of mycelia after inoculation and culture, add cell wall dissolving enzyme preparation and graphene oxide, separate enzyme preparation into 5 g of cellulase, 0.2 g of graphene oxide, dissolve in 100 ml of water, and the cellulase is β-1 , 4-glucan-4-glucan hydrolase, oscillate evenly at a low frequency in an ultrasonic device, and obtain a protoplast solution after sterilization;

[0063] Step c, take 0.6ml of protoplast solution and inoculate it on the surface of the si...

Embodiment 2

[0068]Step a, in a sterile environment, take out the germs, inoculate them in mycelium medium, and culture them statically at 20°C for 10 days; the mycelium medium contains the following weight of raw materials per 1000ml: 45g of maltose, Peptone 15g, add water to make up to 1000ml. The preparation method is: put 1000ml of water in the pot, add peptone and maltose, then add water to make up to 1000ml, put it in a sealed bottle, and sterilize it in an autoclave at 110°C for 25 minutes;

[0069] Step b, take 5 g of mycelia after inoculation and culture, add cell wall dissolving enzyme preparation and graphene oxide, separate enzyme preparation as cellulase 10 g, graphene oxide 1 g, dissolve in 100 ml of water, cellulase is β-1, 4-glucan-4-glucan hydrolase, oscillate evenly at a low frequency in an ultrasonic device, and obtain a protoplast solution after sterilization;

[0070] Step c, take 0.8ml of protoplast solution and smear and inoculate on the surface of the single-cell m...

Embodiment 3

[0075] Step a, in a sterile environment, take out the germs, inoculate them in mycelium medium, and culture them statically at 20°C for 9 days; the mycelium medium contains the following weight of raw materials per 1000ml: 40g of maltose, Peptone 12g, add water to make up to 1000ml. The preparation method is: put 1000ml of water in the pot, add peptone and maltose, then add water to make up to 1000ml, put it in a sealed bottle, and sterilize it in an autoclave at 110°C for 25 minutes;

[0076] Step b, take 5g of mycelium after inoculation and culture, add cell wall dissolving enzyme preparation and graphene oxide, separate enzyme preparation as cellulase 8g, graphene oxide 0.8g, dissolve in 100ml water, cellulase is β-1 , 4-glucan-4-glucan hydrolase, oscillate evenly at a low frequency in an ultrasonic device, and obtain a protoplast solution after sterilization;

[0077] Step c, take 0.8ml of protoplast solution and inoculate it on the surface of the single-cell medium, plac...

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Abstract

The invention provides a breeding process of an enoki mushrooms liquid strain. The process is as follows: in a sterile environment, taking out a strain, inoculating the strain with a mycelial culturemedium, and performing standing culturing at 20 DEG C for 8-10 days. Each 1000ml of the mycelial culture medium contains the following raw materials: 30-45g of maltose and 10-15g of peptone, and wateris added to a constant volume of 1000ml. A preparation method is as follows: adding 1000ml of water to a pot, adding the peptone and the maltose, then adding water to a constant volume of 1000ml, placing the strain in a sealed bottle, and performing sterilization treatment in an autoclave at 110 DEG C for 25 minutes.

Description

technical field [0001] The invention relates to the technical field of breeding of edible fungus strains, in particular to a process for breeding liquid strains of Flammulina velutipes. Background technique [0002] Flammulina velutipes scientific name hair handle money fungus, also known as hair handle small fire mushroom, structure bacteria, port mushroom, winter mushroom, park wild rice, frozen fungus, golden mushroom, wisdom mushroom and so on. Because of its slender stipe, like day lily, it is called Flammulina velutipes, which belongs to the genus Flammulina velutipes of the family Agaricaceae, and is a kind of algae lichen. Flammulina velutipes has a high medicinal and therapeutic effect. [0003] Flammulina velutipes is not only a delicious food, but also a better health food. The domestic and foreign markets of Flammulina velutipes are increasingly broad. The artificial cultivation technology of Flammulina velutipes is not complicated, as long as the environmental...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12R1/645
CPCC12N1/14C12N13/00
Inventor 陈福永樊玲玲林启建张瑜
Owner SHANDONG YOUHE BACTERIA IND CO LTD
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