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Method for preparing cycloastragenol through microorganism mixed fermentation, transformation and degradation of astragaloside iv

The technology of astragaloside IV and cycloastragaloside is applied in the field of medicine and chemical industry, and can solve the problems of high conversion rate, environmental pollution and high cost, and achieve the effect of high conversion rate

Inactive Publication Date: 2019-04-12
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to adopt the method for microbial conversion to overcome the shortcomings of many by-products, high cost, and environmental pollution in the existing chemical and enzymatic preparations of cycloastragenol, and to prepare cycloastragenol by fermentation than the existing microbial fermentation. high rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present embodiment prepares cycloastragenol according to the following steps:

[0025] (1) Seed cultivation

[0026] Put the preserved strains Fusarium verticillioides 0213 (CGMCC NO.16481) and Fusarium proliferatum 0821 (CGMCC NO.16482) into 100mL Erlenmeyer flasks filled with wort medium respectively, and in Cultivate for 48 hours under the conditions of 25° C. and 180 r / min to obtain seed liquid. The composition of wort medium is: glucose 10g / L, peptone 4g / L, yeast extract powder 3g / L, malt powder 10g / L.

[0027] (2) Transformation culture

[0028] Inoculate 10% of the seed solution cultivated in step (2) into 100 mL Erlenmeyer flasks containing fermentation medium, and culture at 25°C and 180 r / min for 6 days to obtain fermentation liquid. The composition of the fermentation medium is: astragalus powder 2g / L, peptone 5g / L, yeast powder 2g / L, K 2 HPO 4 1g / L, MgS0 4 •7H 2 0 0.5g / L, 0.2g / L of astragaloside IV substrate of 50% mass percent.

[0029] (3) Sep...

Embodiment 2

[0033] The present embodiment prepares cycloastragenol according to the following steps:

[0034] (1) Seed cultivation

[0035] Put Fusarium verticillioides 0213 (CGMCC NO.16481) and Fusarium proliferatum 0821 (CGMCC NO.16482) into 100mL Erlenmeyer flasks containing wort medium respectively, at 30°C , 180r / min under the condition of culturing for 36h to obtain seed liquid. The composition of wort medium is: glucose 10g / L, peptone 4g / L, yeast extract powder 3g / L, malt powder 10g / L.

[0036] (2) Transformation culture

[0037] Inoculate 10% of the seed solution cultured in step (2) into 100 mL Erlenmeyer flasks filled with fermentation medium, and culture at 30°C and 180 r / min for 6 days to obtain fermentation liquid. The composition of the fermentation medium is: astragalus powder 2g / L, peptone 5g / L, yeast powder 2g / L, K 2 HPO 4 1g / L, MgS0 4 •7H 2 0 0.5g / L, 0.2g / L of astragaloside IV substrate of 50% mass percent.

[0038] (3) Separation and purification

[0039] Add ...

Embodiment 3

[0042] The present embodiment prepares cycloastragenol according to the following steps:

[0043] (1) Seed cultivation

[0044] Put Fusarium verticillioides 0213 (CGMCC NO.16481) and Fusarium proliferatum 0821 (CGMCC NO.16482) into 100mL Erlenmeyer flasks containing wort medium respectively, at 30°C , 180r / min under the condition of culturing for 36h to obtain seed liquid. The composition of wort medium is: glucose 10g / L, peptone 4g / L, yeast extract powder 3g / L, malt powder 10g / L.

[0045] (2) Transformation culture

[0046] Inoculate 10% of the seed solution cultivated in step (2) into 100 mL Erlenmeyer flasks filled with fermentation medium, and culture at 30°C and 180 r / min for 6 days to obtain fermentation liquid. The composition of the fermentation medium is: astragalus powder 2g / L, peptone 5g / L, yeast powder 2g / L, K 2 HPO 4 1g / L, MgS0 4 •7H 2 0 0.5g / L, 0.2g / L of astragaloside IV substrate in 100% mass percent.

[0047] (3) Separation and purification

[0048] A...

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Abstract

The invention relates to a method for preparing cycloastragenol through microorganism mixed fermentation, transformation and degradation of astragaloside iv. Two strains of fusarium proliferatum 0821(CGMCC NO.16482 ) and Fusarium verticillioides 0213 (CGMCC NO.16481 ) which are screened out are used, the astragaloside iv is used as a substrate, and through mixed fermentation of the two strains, the cycloastragenol is prepared. A technique for mixed fermentation with two microorganisms is adopted to degrade the astragaloside iv so as to obtain the cycloastragenol high in purity. The technological flow is simple, the cost is low, and the entire technological process is performed at normal temperature and normal pressure. The required equipment condition is low, the method is suitable for mass production, the conversion rate of the final cycloastragenol is as high as 80% or above, and the purity is as high as 95% or above.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, and in particular relates to a method for preparing cycloastragenol by microbial mixed fermentation transformation and degradation of astragaloside IV. Background technique [0002] Astragalus is the dried root of Astragalus mongolica or Astragalus membranaceus, a leguminous plant, and is one of the most commonly used medicines in clinical treatment of traditional Chinese medicine. The active ingredients in Astragalus include flavonoids, saponins, polysaccharides and amino acids. Astragaloside IV is a representative compound of astragaloside, which has various functions such as improving immunity, scavenging free radicals, lowering blood pressure and antibacterial. And cycloastragenol is the aglycone of astragaloside, the molecular formula is C 30 h 50 o 5 , The relative molecular mass is 490.71, which is the main product of astragaloside IV metabolism in the intestinal tract, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/77
CPCC12P33/20
Inventor 张鹏潘弘哲陈毅明李佳龙
Owner BEIJING UNIV OF CHEM TECH
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