Experimental device and method for simulating in-vivo hydrodynamic environment

Abstract

The invention relates to an experimental device and method for simulating an in-vivo hydrodynamic environment, and belongs to the field of biomedicine. The components of the device include a flat flowsmall chamber device, a timing switch, a peristaltic pump, a vacuum pump, a heating washer, a liquid storage bottle and a water bath pot. The invention provides the experimental device and method forsimulating the in-vivo hydrodynamic environment; additional carbon dioxide supply is not need to maintain the physiological pH value, and fluid shear stress stimulation is loaded to cells and other culture materials stably; the whole device does not need to be placed in an incubator for operation, on one hand, the operation difficulty is greatly reduced, on the other hand, the problem of instability of gas supply by gas cylinders is well solved, and the repeatability of the whole experiment is good.

Description

technical field [0001] The invention relates to an experimental device and method for simulating a fluid dynamic environment in a body, belonging to the field of biomedicine. Background technique [0002] The effect of fluid shear stress on vascular endothelial cells has made great progress in the field of cardiovascular research. Vascular endothelial cells are in the continuous flow of blood, and thus are subjected to fluid shear stress generated by blood flow. Studies have found that fluid shear stress can affect the arrangement, shape, gene expression, and expression of cell surface adhesion molecules of vascular endothelial cells; studies in other fields have also introduced parallel plate flow chambers. Michael J. Mitchell found that fluid shear stress in the physiological range can also regulate receptor-mediated apoptosis of tumor cells. AnkenyRF believes that fluid shear stress may be a factor that induces the differentiation of endothelial progenitor cells into en...

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Problems solved by technology

[0004] However, the above fluid shear stress devices need to be placed in the incubator to provide the CO2 culture environment required for cell growth, which not only greatly increases the difficulty of experimental operation, but also greatly increases the contamination rate of cells

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