Dendrobium officinale glucomannan
A technology of Dendrobium officinale and glucomannan, which is used in drug combinations, organic active ingredients, anti-tumor drugs, etc., can solve the problem that the active target and mechanism of action are rarely studied, the research on the mechanism of polysaccharide metabolism is not in-depth, and the relationship between polysaccharide and intestinal tract The relationship between bacterial microenvironment and biological function is not very clear, etc., to achieve the effect of good anti-melanoma cell activity and good application prospects
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Embodiment 1
[0043] Embodiment 1: the extraction of dendrobium officinale polysaccharide:
[0044]S1. Squeeze the stem section of Dendrobium candidum with warm water, then treat it overnight with absolute ethanol, wash the precipitate with 80% ethanol, acetone, and anhydrous ether successively, and dry it to obtain the crude polysaccharide (CPS) of Dendrobium candidum;
[0045] S2. After the CPS is hydrolyzed by α-amylase, and then treated overnight with absolute ethanol, the precipitate is dissolved, dialyzed, concentrated, and dried to obtain Dendrobium officinale polysaccharide;
[0046] S3. use DEAE-cellulose column and Sephadex G-200 column chromatography to separate and purify the fine polysaccharide of Dendrobium candidum, and obtain the pure polysaccharide of Dendrobium candidum, i.e. the Dendrobium candidum glucomannan (POW) described in claim 1 .
[0047] Specifically, the Dendrobium officinale glucomannan is prepared by the following method:
[0048] S11. Take the stem section...
Embodiment 2
[0069] Embodiment 2: Determination of polysaccharide content
[0070] 1) Make glucose standard curve: accurately prepare 0.1mg / mL glucose standard solution, absorb 0.0, 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0mL respectively, make up to 1.0mL with distilled water, and add 5% in ice water 1mL of phenol solution, mix well, precisely add 5mL of concentrated sulfuric acid, shake well, let stand for 5min, place in boiling water bath and heat for 20min, take it out, let stand and cool to room temperature, measure the absorbance at 490nm, repeat the test 3 times. Standard curve made with glucose standard: y=12.638x -0.0061, R 2 =0.9999 (x is glucose concentration, y is absorbance value).
[0071] 2) Determination of the polysaccharide content of the sample: Precisely measure 1mL of the sample solution, operate according to the above steps, measure the absorbance value, and calculate the polysaccharide content according to the standard curve. The results of determination of crude polysacchar...
Embodiment 3
[0074] Embodiment 3: Identification of Dendrobium candidum glucomannan purity
[0075] 1. Since polysaccharides are macromolecular compounds, their purity standards are different from those of small molecules (molecular weight less than 2000Da), that is, the purity of polysaccharides only represents the average distribution of similar chain lengths of a certain polysaccharide. High performance gel permeation chromatography (HPGPC) is the detection method A method of polymer average molecular weight and its distribution and polysaccharide purity. It shows that the more symmetrical the peak shape, the higher the purity of the polysaccharide; the closer the polydispersity coefficient is to 1, the higher the purity of the polysaccharide.
[0076] 2. Use HPGPC to detect the purity of POW:
[0077] Prepare 1.0 mg / mL POW aqueous solution, dissolve in boiling water, cool to room temperature, filter through a 0.45 μm microporous membrane, and analyze by HPGPC.
[0078] Experimental c...
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