Chicken salmonella enteritidis inactivated vaccine and application thereof

A technology of Salmonella enteritidis and Salmonella, which is applied in the field of microorganisms to achieve the effects of easy storage, strong pertinence, and good immune effect

Pending Publication Date: 2019-04-23
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that there is no effective inactivated vaccine to prevent Salmonella enteritidis

Method used

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  • Chicken salmonella enteritidis inactivated vaccine and application thereof
  • Chicken salmonella enteritidis inactivated vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A chicken Salmonella inactivated vaccine obtained through the following steps:

[0021] The adjuvant is composed of the following raw materials in parts by weight: 50 parts of water-based oil adjuvant, 1.5 parts of Span-80, 2.5 parts of Tween-80, 0.1 part of small peptide, and 1.5 parts of propolis.

[0022] Expand the culture of Salmonella, inactivate, and store at 4°C for later use; prepare the oil phase adjuvant: weigh the water-based oil adjuvant and Span-80 according to the weight ratio, heat until the solids are completely dissolved, and mix evenly; prepare the water phase: Dilute the inactivated Salmonella to a concentration greater than 5 x 10 9 CFU / mL, then add the adjuvant Tween-80, propolis, and small peptides into the bacterial solution, and mix well. The inactivated Salmonella vaccine can be obtained by emulsifying the oil phase and the water phase at a ratio of 1:1.

Embodiment 2

[0024] A chicken Salmonella inactivated vaccine obtained through the following steps:

[0025] The adjuvant is composed of the following raw materials in parts by weight: 50 parts of water-based oil adjuvant, 3 parts of Span-80, 2.0 parts of Tween-80, 0.1 part of small peptide, and 1.5 parts of propolis.

[0026] Expand the culture of Salmonella, inactivate, and store at 4°C for later use; prepare oil phase adjuvants: weigh 50 parts of water-based oil adjuvants according to the weight ratio, Span-80, heat until the solids are completely dissolved, and mix well; prepare water Phase: Dilute the inactivated Salmonella to a concentration greater than 5 x 10 9 CFU / mL, then add the adjuvant Tween-80, propolis, and small peptide into the bacterial solution, and mix well. The inactivated Salmonella vaccine can be obtained by emulsifying the oil phase and the water phase at a ratio of 1:1.

Embodiment 3

[0028] A chicken Salmonella inactivated vaccine obtained through the following steps:

[0029] The adjuvant is composed of the following raw materials in parts by weight: 50 parts of water-based oil adjuvant, 3.5 parts of Span-80, 1.5 parts of Tween-80, 0.1 part of small peptide, and 2.5 parts of propolis.

[0030] Expand the culture of Salmonella, inactivate, and store at 4°C for later use; prepare oil phase adjuvants: weigh 50 parts of water-based oil adjuvants according to the weight ratio, Span-80, heat until the solids are completely dissolved, and mix well; prepare water Phase: Dilute the inactivated Salmonella to a concentration greater than 5 x 10 9 CFU / mL, then add the adjuvant Tween-80, propolis, and small peptide into the bacterial solution, and mix well. The inactivated Salmonella vaccine can be obtained by emulsifying the oil phase and the water phase at a ratio of 1:1.

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PUM

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Abstract

The invention relates to the technical field of microorganisms, in particular to a poultry salmonella vaccine, and further relates to application of salmonella. The chicken salmonella enteritidis inactivated vaccine composition comprises chicken salmonella enteritidis liquid and an adjuvant, and the adjuvant is prepared from, by weight, 50 parts of a water-based oil adjuvant, 1.5-3.5 parts of Span-80, 1.5-2.5 parts of Tween-80, 0.1 part of small peptide and 1.5-2.5 parts of propolis. The enteritidis inactivated vaccine is high in pertinency, the immune effect on locally epidemic salmonella strains, the preparation process is easy to operate, the cost is low, storage is easy, and the chicken salmonella enteritidis inactivated vaccine is suitable for large-scale farms with laboratories.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a salmonella vaccine for poultry, and also relates to the application of the salmonella. Background technique [0002] With the intensive and large-scale development of my country's breeding industry, the incidence of livestock and poultry diseases is increasing year by year, and new diseases are increasing. question. So far, Salmonella infection in poultry is relatively common, most of which are pullorum type and enteritis type. Antibiotics are the most effective means to prevent and control Salmonella. Prophylactic and therapeutic effects of antibiotics on Salmonella infection. In order to improve the curative effect, the dosage of clinical antibiotics is higher than the recommended dosage, which leads to the problem of antibiotic residues in animal products. Therefore, new alternative methods or products of antibiotics are urgently needed in the clinical prevention and treat...

Claims

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Application Information

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IPC IPC(8): A61K39/112A61K39/39A61P31/04C12N1/36C12N1/20C12R1/42
CPCA61K39/0275A61K39/39A61P31/04C12N1/20C12N1/36A61K2039/55588A61K2039/55583A61K2039/55516A61K2039/521
Inventor 骆延波刘玉庆齐静胡明李璐璐张印张庆赵效南
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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