A method for extracting peony seed oil and peony protein peptide by enzymatic hydrolysis

A technology of peony seed oil and peony seeds, which is applied in the field of food and peptide or protein preparation, can solve the problems of long microbial fermentation time, poor combination effect, cumbersome procedures, etc., and achieve improved enzymatic hydrolysis efficiency, product activity, and transparent color Bright, good-tasting effect

Active Publication Date: 2021-04-02
山东贝世康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The peony protein peptides in the prior art are generally prepared by microbial fermentation and / or protease, but the effect of using protease and microbial fermentation alone is poorer than the combination of the two, and microbial fermentation takes a long time and the procedure is cumbersome. Higher yields require combined treatment with multiple types of enzymes at higher cost
[0004] In recent years, the deep processing of peony seed food has become more and more widely used. There are many studies on the extraction of peony seed oil and active ingredients, but there is no report on the simultaneous extraction of peony seed oil and peony protein peptides by using ultrasonic technology to assist water enzymatic hydrolysis.

Method used

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  • A method for extracting peony seed oil and peony protein peptide by enzymatic hydrolysis
  • A method for extracting peony seed oil and peony protein peptide by enzymatic hydrolysis
  • A method for extracting peony seed oil and peony protein peptide by enzymatic hydrolysis

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Effect test

Embodiment 1

[0029] A method for extracting peony seed oil and peony protein peptide by a water enzymatic hydrolysis method, comprising the following steps:

[0030] (1) Preparation of peony seed kernel powder: the peony seed kernels are collected after the peony seeds are broken, pulverized, passed through a 40-mesh sieve, and treated with a microwave of 8kW power for 30 min under vacuum conditions of 50° C. to obtain peony seed kernels. pink.

[0031] (2) Wall breaking: the peony seed kernel powder obtained in step (1) is subjected to wall breaking with an ultrafine airflow pulverizing classifier, and the wall breaking time is 25min.

[0032] (3) Ultrasonic enzymolysis: adding water to the broken peony seed kernel powder obtained in step (2), the amount of water added is 6 times the weight of the peony seed kernel powder, and then adding alkaline protease, the amount of alkaline protease added It is 0.026% of the weight of peony seed kernel powder, and the protease is Alcalas 2.4L. Ult...

Embodiment 2

[0038] A method for extracting peony seed oil and peony protein peptide by a water enzymatic hydrolysis method, comprising the following steps:

[0039] (1) Preparation of peony seed kernel powder: the peony seed kernels are collected after the peony seeds are broken, pulverized, passed through a 40-mesh sieve, and treated with microwaves with a power of 10 kW for 30 min under vacuum conditions of 40° C. to obtain peony seed kernels. pink.

[0040] (2) Wall breaking: the peony seed kernel powder obtained in step (1) is subjected to wall breaking with an ultrafine airflow pulverizing classifier, and the wall breaking time is 30min.

[0041] (3) Ultrasonic enzymolysis: adding water to the broken peony seed kernel powder obtained in step (2), the amount of water added is 7 times the weight of the peony seed kernel powder, and then adding alkaline protease, the amount of alkaline protease added It is 0.03% of the weight of peony seed kernel powder, and the protease is Ns37071. U...

Embodiment 3

[0047] A method for extracting peony seed oil and peony protein peptide by a water enzymatic hydrolysis method, comprising the following steps:

[0048] (1) Preparation of peony seed kernel powder: the peony seed kernels are collected after the peony seeds are broken, pulverized, passed through a 40-mesh sieve, and treated with a microwave of 8kW power for 30 min under vacuum conditions of 30° C. to obtain peony seed kernels. pink.

[0049] (2) Wall breaking: the peony seed kernel powder obtained in step (1) is subjected to wall breaking with an ultrafine airflow pulverizing classifier, and the wall breaking time is 20min.

[0050] (3) Ultrasonic enzymolysis: adding water to the broken peony seed kernel powder obtained in step (2), the amount of water added is 8 times the weight of the peony seed kernel powder, and then adding alkaline protease, the amount of alkaline protease added It is 0.03% of the weight of peony seed kernel powder, and the protease is 0.02% of Ns37071 an...

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Abstract

The invention discloses a method for extracting peony seed oil and peony protein peptide by water enzymatic hydrolysis, which belongs to the technical field of preparation of foods and peptides or proteins. The peony seed kernel powder is prepared, and is subjected to wall breaking by a superfine airflow pulverizing classifier, and the peony seed oil is obtained by ultrasonic enzymatic hydrolysis,centrifugal layering, and vacuum rotary evaporation and drying; after the filter residue is ultrasonically extracted, the aqueous layers are combined, after vacuum condensation, and spray drying is carried out to obtain the peony protein peptide powder. The peony seed oil and peony protein peptide can be extracted at the same time. The oil yield of peony seed is 90%, and the recovery rate of peony protein peptide is 86%. The preparation method of peony seed oil by ultrasonic assisted water enzymatic hydrolysis is safe, green and energy-saving. Pulsed ultrasonic treatment is used in the preparation of protein peptides to improve enzymatic efficiency and product activity. The prepared peony seed oil has relatively high unsaturated fatty acid content, short extraction time, and no solvent residue, and the DPPH removal value is reduced by 15% to 38%.

Description

technical field [0001] The invention belongs to the technical field of preparation of food and peptides or proteins, and particularly relates to a method for extracting peony seed oil and peony protein peptides by a water enzymatic hydrolysis method. Background technique [0002] Peony seeds have the functions of anti-inflammatory, regulating cardiovascular system, protecting liver, lowering blood sugar and regulating immune function. The oil content of peony seeds (18-25%) is higher than that of soybeans (17-18%), and the fatty acid composition of peony seed oil better than soybean oil. Peony seed oil was approved as a new resource food by the Ministry of Health in March 2011 "Announcement of the Ministry of Health on Approving Yuanbao Maple Seed Oil and Peony Seed Oil as New Resource Foods" (No. 9, 2011), containing more than 100 active substances , rich in unsaturated fatty acids (about 80%), mainly linolenic acid and linoleic acid. As the main source of "alpha-linoleni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K2/00C07K1/34C11B1/00
Inventor 吴震生李相振杨曜华姚军朋林成利
Owner 山东贝世康生物科技有限公司
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