A method for extracting peony seed oil and peony protein peptide by enzymatic hydrolysis

Abstract

The invention discloses a method for extracting peony seed oil and peony protein peptide by water enzymatic hydrolysis, which belongs to the technical field of preparation of foods and peptides or proteins. The peony seed kernel powder is prepared, and is subjected to wall breaking by a superfine airflow pulverizing classifier, and the peony seed oil is obtained by ultrasonic enzymatic hydrolysis,centrifugal layering, and vacuum rotary evaporation and drying; after the filter residue is ultrasonically extracted, the aqueous layers are combined, after vacuum condensation, and spray drying is carried out to obtain the peony protein peptide powder. The peony seed oil and peony protein peptide can be extracted at the same time. The oil yield of peony seed is 90%, and the recovery rate of peony protein peptide is 86%. The preparation method of peony seed oil by ultrasonic assisted water enzymatic hydrolysis is safe, green and energy-saving. Pulsed ultrasonic treatment is used in the preparation of protein peptides to improve enzymatic efficiency and product activity. The prepared peony seed oil has relatively high unsaturated fatty acid content, short extraction time, and no solvent residue, and the DPPH removal value is reduced by 15% to 38%.

Description

technical field [0001] The invention belongs to the technical field of preparation of food and peptides or proteins, and particularly relates to a method for extracting peony seed oil and peony protein peptides by a water enzymatic hydrolysis method. Background technique [0002] Peony seeds have the functions of anti-inflammatory, regulating cardiovascular system, protecting liver, lowering blood sugar and regulating immune function. The oil content of peony seeds (18-25%) is higher than that of soybeans (17-18%), and the fatty acid composition of peony seed oil better than soybean oil. Peony seed oil was approved as a new resource food by the Ministry of Health in March 2011 "Announcement of the Ministry of Health on Approving Yuanbao Maple Seed Oil and Peony Seed Oil as New Resource Foods" (No. 9, 2011), containing more than 100 active substances , rich in unsaturated fatty acids (about 80%), mainly linolenic acid and linoleic acid. As the main source of "alpha-linoleni...

AI Technical Summary

Problems solved by technology

The peony protein peptides in the prior art are generally prepared by microbial fermentation and / or protease, but the effect of using protease and microbial fermentation alone is poorer than the combination of the two, and microbial fermentation takes a long time and the procedure is cumbersome. Higher yields require combined treatment with multiple types of enzymes at higher cost

[0004] In recent years, the deep processing of peony seed food has become more and more widely used. There are many studies on the extraction of peony seed oil and active ingredients, but there is no report on the simultaneous extraction of peony seed oil and peony protein peptides by using ultrasonic technology to assist water enzymatic hydrolysis.

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