Preparation method of improved reagent for measuring isoenzyme content of creatine kinase

Abstract

The invention discloses a preparation method of an improved reagent for measuring isoenzyme content of creatine kinase. An R1 reagent and an R2 reagent are included; the R1 reagent consists of imidazole, D(+)-glucose, diethylenetriaminepentaacetic acid, sodium sulfite, magnesium acetate, ADP-K, AMP, N-acetylcysteine, NaDP-Na, A5-PP, NaN3, hexokinase and G6-P-DH; and the R2 reagent consists of CAPSO free acid, D(+)-glucose, CREATINE PHOSPHATE and NaN3. The reagent adopts an immunosuppression method and uses anti-human M serum to inhibit M subunits, so that residual B subunit activity can be determined, and CK-MB activity can be obtained by measuring the B subunit activity under a wavelength of 340 nm and then performing corresponding operation; and the reagent directly adopts a CKMB antibody to perform direct reaction, so that interference in the prior art brought during a series of processing can be avoided, and therefore, monitoring data precision can be increased.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for preparing an improved reagent for measuring the content of creatine kinase isozyme. Background technique [0002] Creatine kinase isoenzymes are of great significance in clinical diagnosis. When various lesions including muscle atrophy and myocardial infarction occur, the level of creatine kinase in human serum increases rapidly. The activity of acid kinase is more reliable than electrocardiogram. During myocardial infarction, creatine kinase rises within 6 hours of onset, reaches a peak within 24 hours, and returns to normal within 3-4 days. Among them, the creatine kinase isoenzyme CK-MB The diagnostic specificity is the highest. [0003] Biochemical analyzers are currently used to measure creatine kinase isoenzymes: CK is composed of two subunits M and B to form three dimers, and CK1 (BB), CK2 (MB) and CK3 (MM) mainly exist in the cytoplasm In addition, there i...

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Problems solved by technology

[0003] Biochemical analyzers are currently used to measure creatine kinase isoenzymes: CK is composed of two subunits M and B to form three dimers, and CK1 (BB), CK2 (MB) and CK3 (MM) mainly exist in the cytoplasm In addition, there is a small amount of mitochondrial isoenzyme (CKm) in the serum, and the activity of CK in the myocardium is relatively high (second only to skeletal muscle), of which CK-MB accounts for about 13-22% (<1% in skeletal muscle), Therefore, it has specificity as an indicator of myocardial injury. During the determination of creatine kinase isoenzyme by biochemical analyzer, the measurement method of blocking CKM antibody and calculating the CKMB content will produce certain interference during the test process, resulting in Distortion of detection results

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