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Microparticle chemiluminescence detection kit for immunoassay of antiviral protein MxA

A technology of chemiluminescence immunity and detection kits, which is applied in the direction of chemiluminescence/bioluminescence, analysis by causing chemical reactions of materials, and analysis of materials, which can solve the problems that cannot be widely used in clinical diagnosis and scientific research work, and limit antiviral proteins. The popularization and application of MxA, the susceptibility to human operation and the interference of external factors, etc., achieve the effects of stable results, shortened reaction time and high sensitivity

Inactive Publication Date: 2019-05-07
XIAMEN INNODX BIOTECH CO LTD +1
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Problems solved by technology

At present, the clinical diagnostic technology of antiviral protein MxA mainly includes: enzyme-linked immunoassay (ELISA), but because of cumbersome operation, time-consuming, easy to be interfered by human operation and external factors, and low degree of automation, the detection of antiviral protein MxA is limited. The popularization and application of it prevents it from being widely used in clinical diagnosis and scientific research

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  • Microparticle chemiluminescence detection kit for immunoassay of antiviral protein MxA
  • Microparticle chemiluminescence detection kit for immunoassay of antiviral protein MxA
  • Microparticle chemiluminescence detection kit for immunoassay of antiviral protein MxA

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Embodiment 1

[0035] An antiviral protein MxA microparticle chemiluminescent immunoassay detection kit, including coating reaction buffer, magnetic microparticle solution, second antiviral protein MxA monoclonal antibody solution, first luminescence liquid, second luminescence liquid, whole blood lysate , different concentrations of antiviral protein MxA antigen standard solution and concentrated washing solution. Its use method includes the following steps:

[0036] (1) Take the whole blood to be tested, add the above-mentioned whole blood lysate to it, lyse and centrifuge to obtain the supernatant;

[0037] (2) adding the above-mentioned magnetic microparticle solution to the above-mentioned supernatant for reaction, then washing with the above-mentioned concentrated washing solution after dilution, and then adding the above-mentioned second antiviral protein MxA monoclonal antibody solution for reaction;

[0038] (3) Wash the material obtained in step (2) with the above-mentioned concen...

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Abstract

The invention discloses a microparticle chemiluminescence detection kit for immunoassay of antiviral protein MxA, which comprises a coating reaction buffer, a magnetic microparticle solution, a secondantiviral protein MxA monoclonal antibody solution, a first luminescent liquid, a second luminescent liquid, a whole blood lysing solution, different concentrations of antiviral protein MxA antigen standard solution and concentrated washing solution. The invention fills the gap in the production of microparticle chemiluminescence detection reagent for immunoassay of antiviral protein MxA in China, which has the advantages of simple operation, high sensitivity, wide linear range, stable results, good safety, easy automation, and has broad application prospects in clinical testing.

Description

technical field [0001] The invention belongs to the technical field of chemiluminescence detection kits, in particular to a chemiluminescence immunoassay detection kit of antiviral protein MxA microparticles. Background technique [0002] The molecular weight of Mx protein is about 70-80kD, and its protein structure is composed of at least three regions: N-terminal domain, middle domain, and C-terminal domain. The N-terminal domain consisting of about 300 amino acids is a GTPase The active region, the C-terminal domain consisting of about 100 amino acids is the effector region of the GTPase, and the middle domain is composed of about 150 amino acids. The MxA protein is connected to the smooth endoplasmic reticulum in the cytoplasm, and the configuration changes after combining with the nucleocapsid of the virus. The nucleocapsid bound to the MxA protein is deposited around the nucleus in the form of a fibrous complex, and the activated The MxA protein causes the virus to lo...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/68G01N33/58G01N33/577G01N33/543G01N33/532G01N21/76
Inventor 陈自敏熊君辉徐伟玲王龙童勋章葛胜祥孙旭东
Owner XIAMEN INNODX BIOTECH CO LTD
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