Tea tree hexose transporter gene CsSWETE17 and application thereof in regulating and controlling plant vegetative growth and seed size

A technology of vegetative growth and transporter, applied in the field of biological genetic engineering, can solve problems such as unreported functions

Active Publication Date: 2019-05-14
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The CsSWEET17 gene sequence involved in the present invention has been published in NCBI (accession number: KP025705), but its function has not been reported

Method used

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  • Tea tree hexose transporter gene CsSWETE17 and application thereof in regulating and controlling plant vegetative growth and seed size
  • Tea tree hexose transporter gene CsSWETE17 and application thereof in regulating and controlling plant vegetative growth and seed size
  • Tea tree hexose transporter gene CsSWETE17 and application thereof in regulating and controlling plant vegetative growth and seed size

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Cloning of CsSWEET17 Gene of Tea Tree and Construction of Overexpression Vector

[0027] According to the CsSWEET17 sequence disclosed in the NCBI database, its nucleotide sequence is shown in SEQ ID NO: 1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO: 2, primers F and R were designed, and the tea tree leaf cDNA was used as a template , the gene CsSWEET17 was amplified by PCR. The amplified sequence was connected to the pENTRTM / D-TOPO vector, and finally connected to the pH7FWG2 plant expression vector through LR reaction.

[0028] The primers used were F: 5'-CACCATGGCTAGCTTGAGCTTCATC-3'; R: 5'-AGGGTGATCCTTGGTGCTTCC-3'.

Embodiment 2

[0030] Construction and Screening of CsSWEET17 Gene Overexpression Plants

[0031] The sequence-correct vector constructed in Example 1 was transformed into the Agrobacterium strain GV3101, and then transformed into the wild-type Arabidopsis plant to obtain the seeds of the transgenic contemporary seedling of Arabidopsis thaliana. The screening resistance gene carried by the pH7FWG2 vector is hygromycin, and the transgenic seedlings of Arabidopsis thaliana were screened with hygromycin. Perform hygromycin resistance screening, and select a strain with a segregation ratio of about 3:1 between resistant seedlings and non-resistant seedlings, indicating that in this strain, the vector sequence with the target gene is inserted in a single copy form. Remove the hygromycin-resistant plants in these lines, harvest the seeds from each plant, and then screen for hygromycin resistance. If there is no separation, it means that the transgenic line is homozygous, and the homozygous can be ...

Embodiment 3

[0034] Vegetative growth phenotypes of plants overexpressing CsSWEET17

[0035] After the Arabidopsis seeds were sterilized, they were sown on 1 / 2 MS medium, and after 7 days of growth, they were moved into seedling blocks for growth. When cultivating to seedling age is 13 days and 18 days, take pictures and record respectively, see figure 2 and image 3. The 18-day-old seedlings were also weighed for aboveground fresh weight (the wild type and OE-1, 15 seedlings of OE-9 were counted respectively), see Figure 4 . The results showed that OE-1 and OE-9 overexpression plants both showed increased leaf area and increased fresh weight of aerial parts compared with the wild type.

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Abstract

The invention relates to a tea tree hexose transporter gene CsSWETE17 and application thereof in regulating and controlling plant vegetative growth and seed size, belonging to the technical field of biological genetic engineering. The nucleotide sequence of the tea tree hexose transporter gene CsSWETE17 is shown in SEQ ID NO. 1; wherein the protein sequence is shown as SEQ ID NO. 2. According to the tea tree hexose transporter gene CsSWETE17 and the application of the tea tree hexose transporter gene CsWETE17 in regulating and controlling plant vegetative growth and seed size, proteins coded by the CsWETE17 gene have conservation and important biological functions in different plants. The tea tree hexose transporter gene CsSWETE17 and application thereof in regulating and controlling plantvegetative growth and seed size provides an important and possibly universal gene resource for regulating and controlling plant vegetative growth and seed size, and provides excellent candidate genesfor cultivating varieties for leaf use and seed use.

Description

technical field [0001] The invention belongs to the technical field of biological gene engineering, in particular to the tea tree hexose transporter gene CsSWEET17 and its application in regulating plant vegetative growth and seed size. Background technique [0002] Leaf is an important vegetative organ of plants and a place for photosynthesis. Leaf area can affect the yield of crops to a certain extent. Seed size is a very important agronomic trait and one of the main factors affecting crop yield. Studying the important genes controlling leaf vegetative growth and seed size, and analyzing the regulatory molecular mechanism of leaf and seed size can lay an important foundation for high-yield crop breeding, and has important practical application value in agricultural production. Tea tree is an important economic crop mainly for leaves. At the same time, tea seeds of some tea tree resources can be used to extract oil. The development and leaf size of tea tree leaves directly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20
Inventor 王璐姚利娜王新超杨亚军郝心愿
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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