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Blattella germanica knockdown resistance-related mutant gene, protein and identification primer and identification method

A german cockroach, resistance-related technology, applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problem of german cockroach's serious drug resistance, achieve intuitive and enhanced results Resistance, fast and accurate detection

Inactive Publication Date: 2019-05-14
中国人民解放军东部战区疾病预防控制中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to reduce the impact and harm that German cockroaches bring to people's life, work and health, all kinds of chemical insecticides are mainly used at home and abroad to kill it. However, due to the abuse and misuse of insecticides and the German cockroach The rapid life generation of cockroaches has caused the problem of drug resistance in German cockroaches to become more and more serious

Method used

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  • Blattella germanica knockdown resistance-related mutant gene, protein and identification primer and identification method
  • Blattella germanica knockdown resistance-related mutant gene, protein and identification primer and identification method
  • Blattella germanica knockdown resistance-related mutant gene, protein and identification primer and identification method

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Embodiment 1

[0028] Embodiment 1: German cockroach resistance gene mutation and identification

[0029] (1) German cockroach knockdown time measurement: prepare four kinds of medicines (permethrin, 98% permethrin, 95% tetramethrin, D-trans permethrin, concentration 0.4mg / ml) 150ml respectively, Take 15ml of the prepared medicine, dilute it to 30ml with medical alcohol, and place it in a newly taken paper cup. Wet the clean gauze cut into 50cm×40cm completely, and dry the gauze at room temperature (the final drug concentration is 30mg / m 2 ). Take German cockroaches (11 as a group), buckle them upside down on the dried gauze using a petri dish, observe and record the knockdown status of German cockroaches every 2 minutes, and take out the knocked down German cockroaches in time. Cockroach, placed in a 1.5 ml EP tube. Each drug was tested in two groups, a total of 88 rats.

[0030] (2) The tested German cockroaches (88 cockroaches) were extracted using the DNA extraction kit provided by J...

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Abstract

The invention discloses a blattella germanica knockdown resistance-related mutant gene. A DNA sequence thereof is shown in SEQ ID NO. 1. A protein thereof and an identification primer and an identification method are disclosed. The method amplifies a mutant gene fragment of the blattella germanica knockdown resistance-related gene by a pair of primers and performs sequencing, and identifies a blattella germanica resistance gene by a four-primer ARMS-PCR method. The method can be widely applied to the identification requirements of blattella germanica resistance gene mutation and correspondingscientific research fields.

Description

technical field [0001] The invention relates to a german cockroach knockdown resistance-related mutant gene, protein, identification primer and identification method, and belongs to the field of biotechnology. Background technique [0002] German cockroaches are the most widely distributed and the most difficult to control worldwide household hygiene pests. The reproduction speed of German cockroaches is thousands of times faster than that of ordinary cockroaches. Under suitable conditions, a female can reproduce about 4 generations a year, and after about half a month, the eggs can hatch and grow into adults. Generally, there are several times or even thousands of times more cockroaches. The living habits of German cockroaches are similar to those of common cockroaches, and they like to be active in hotels, Chinese and Western kitchens, bars, restaurants, private rooms and other places. Similar to the hazards of cockroaches, German cockroaches can damage daily necessities...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
Inventor 谭伟龙艾乐乐吕恒杨庆贵吕瑞辰杨露朱长强胡丹丁晨曦
Owner 中国人民解放军东部战区疾病预防控制中心
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